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《Chinese Journal of Cancer》 2002-04
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Cell Cycle Arrest and Apoptosis Induced by Tubeimoside in HeLa Cell

YANG Ping, YU Ting xi, MA Run di, YU Li jian*, SU Wei ming, SHAO Hai yan, LIAO Ming neng, HUANG Lai zhen Guangdong Provincial Key Laboratory of Marine Pharmacy,Zhanjiang Ocean University, Zhanjiang 524025,P.R.China  
Background Objective:Tubeimoside,which is composed of tubeimoside I (79%) and II (21%), was isolated from the tubers of Bolbostemma paniculatum (Maxim) Franquet (Cucurbitaceae), a traditional Chinese medicine, "Tu Bei Mu". This study was designed to investigate the anti tumor mechanism of tubeimoside. Methods:Growth inhibition was measured by MTT assay. Induction of cell cycle arrest and apoptosis was determined by flow cytometry, fluorescence and electron microscopy, and gel electrophoresis of fragmented DNA. Results: Tubeimoside display strong growth inhibitory effect in a dose and time dependant manner against HeLa cells with estimated IC50 values of 20.0, 18.8,and 8.8 μmol/L after 24,48,and 72 h of treatment with tubeimoside. The flow cytometry profiles revealed that treatment with tubeimoside (5 h;15,30,35 μmol/L) led to a dose dependant shift from 9 80%up to 21 90%,and 27 00%in percentage of cells with a G2/M like DNA content.On the other hand,treatment with tubeimoside(12 h,15,30,35 μmol/L)led to a time dependant shift from 8 20%up to 21 40%,31 15%,and 34 55%,respectively. Exposure of HeLa cells to 40 μmol/L of tubeimoside induced nuclear shrinkage,chromation condensation and margination against nuclear envelope,subdiploid peak,and DNA fragmentation, characteristic as seen in apoptotic cells. Conclusion: Induction of cell cycle arrest and apoptosis may play an important role in the anti tumor effect of tubeimoside.
【CateGory Index】: R737.33
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