Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cancer》 2002-04
Add to Favorite Get Latest Update

Anticancer Effect of Hydroxycampothecin on OralSquamous Carcinoma Cell Line

DING Xue qiang1*, WANG An xun1, KONG Qing yu2, CHEN Hui zhi3, CHEN Yu11 Department of Stomatology,The First Affiliated Hospital,Sun Yat sen University ,Guangzhou 510080,P.R.China2 Department of Medicine,The First Affiliated Hospital,Sun Yat sen Un  
Background Objective: Studies have demonstrated that hydroxycamptothecin (HCPT) had therapeutic effect on many malignant tumors, but few studies on oral squamous cell carcinoma were reported. This study was designed to investigate the anticancer effect and mechanism of hydroxycampothecin (HCPT) on oral squamous carcinoma cell line(Tca8113). Methods: The cytotoxicity of HCPT on Tca8113 cell line was measured by MTT assay and cell cycle detected by flow cytometry(FCM). The growth status of Tca8113 cell xenografts following treatment with HCPT was observed. The doubling times and tumor inhibition rate were calculated. Results: HCPT had strong cytotoxicity on Tca8113 cells, the IC50 was 2 μmol/L. After treatment with low concentration of HCPT, the cell cycle was arrested in S phase or G2+M phase; while at high concentration,apoptosis was obviously found and the rate of apoptosis was increased as the time and concentration of HCPT. Compared with control group, the xenografts of HCPT treated group grew slower and tumor doubling time was prolonged, The tumor volumes of HCPT treated group at 28th day were significantly smaller(P 0 001) with tumor inhibition rate of 69 6%. Conclusion: The result showed that HCPT had strong cytotoxicity effect to oral squamous carcinoma cells and significant growth inhibition on Tca8113 xenografts. HCPT can arrest cell cycle in S phase and G2+M phase and induce cell apoptosis.
【Fund】: 广东省科委攻关项目(2KM04404S)
【CateGory Index】: R739.8
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 9 Hits
1 Chai Li Ping, Su Zhen Zhong, Xian Zhi Xiong Department of Otolaryngology,The First Affiliated Hospital,Sun Yat sen University,Guangzhou,Guangdong, 510080,P.R.China;Inhibition of Hydroxycamptothecin on Laryngeal Squamous Carcinoma Cell Line[J];Chinese Journal of Cancer;2003-04
2 WANG An-Xun1, LI Su2, DING Xue-Qiang1, CHEN Yu1, CHEN Dan1 1. Department of Stomatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510080, P. R. China 2. Skate Key Laboratory of Oncology in Southern China, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P. R. China;Antitumor Effects of Ring-closed and Ring-opened Hydroxycamptothecin on Oral Squamous Carcinoma Cell Line Tca8113[J];Chinese Journal of Cancer;2005-08
3 HE Jie;Nursing Care for Aged Patients with Maxillofacial Squamous Carcinoma Treated by Hydroxycamptothecine and Radiotherapy[J];Journal of Nursing Science;2009-14
4 SHANG Hong-tao, LIU Rui,CHEN Peng,LEI De-lin (Department of Oral and Maxillofacial Surgery, Stomatology College,the Fourth Millitary Medical University,Xi'an 710032,Shaanxi,China);Pingyangmycin inhibit cell activity and telomerase activity on oral carcinoma[J];Chinese Journal of Aesthetic Medicine;2006-06
5 ZHANG Wei-feng,ZHANG Bin,CHEN Shuang-feng(Liaocheng People's Hospital,Liaocheng 252000,P.R.China);Mechanism of hydroxycamptothecin(HCPT) inducing oral squamous cell carcinoma cells apoptosis[J];Shandong Medical Journal;2007-11
6 Chen Peng, Lei Delin, Sun Moyi, et al. College of Stomatology, Fourth Military Medical University, Xi'an 710032;Inhibitary effects of hydroxycampothecin, pingyangmycin, cis-platinum or their combination on the human tongue carcinoma cells[J];Journal of Practical Stomatology;2003-01
7 CHEN Peng 1,LEI De-lin 1,LIU Bing 2,LI Jian-hu 1,SHANG Hong-tao 1 (1.Department of Oral and Maxillofacial Surgery,College of Stomatology,Fourth Millitary Medical University,Xian 710032,China; 2.Department of Prosthodontics,College of Stomatology,Fourth Millitary Medical University,Xian 710032,China);The inhibiting effect of cisplatin on cells and telomerase activity of squamous cell carcinoma of tongue[J];Railway Medical Journal;2003-02
8 LUO Biao,Yao Jinguang,LI Jun,et al. Department of Oncology,Affiliated Hospital of Youjiang Madical College for Nationalities,Baise 533000;Study on the short-term response of HCPT+DDP+5-Fu regimen in the treatment of recurrence and metastasis after surgery of oral squamous cell carcinoma[J];Journal of Modern Stomatology;2004-03
9 LI Jun-ying1, LIU Xiao-ming1, AN Li-jia2, LIN Xi-ran11.Depantment of Dermatology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China, 2.Department of Bioscience and Engineering, Dalian Science and Engineering University, Dalian 116027, China;Effects of a Novel Camptothecin Analogue,Iso-camptothecin on Proliferation and Cell Cycle of Squamous Carcinoma Cell[J];Chinese Journal of Dermatovenerology of Integrated Iraditional and Western Mdeicine;2005-03
Chinese Journal Full-text Database 3 Hits
1 FU Li- wu*, LIANG Yong- ju, PAN Qi- chao Cancer Center, Sun Yat- sen University of Medical Sciences, Guangzhou 510060, P.R.China;Study of Anticancer Effect of Hydroxycampothecin- based Combination against Experimental Nasopharyngeal Carcinoma[J];Chinese Journal of Cancer;2001-04
2 Zhang Qunzhou,Zhou Keyuan,Ling Guangxin.. Institute of Medical Biochemistry of Gungdong Medical College,Zhanjiang,Guangdong Province 524023;Apoptosis of nasopharyngeal carcinoma cells induced by topoisomerase Ⅰ Inhibitor[J];CHINESE JOURNAL OF OTORHINOLARYNGOLOGY;1997-06
3 Fan Jie, Tang Xiaoda, Zhang Xianyou, et al. Department of Urology, Shanghai First People′s Hospital, Shanghai, 200080;10 Hydroxycamptothecine induces apoptosis in human T24 urinary bladder cancer cells[J];NATIONAL MEDICAL JOURNAL OF CHINA;1998-04
Chinese Journal Full-text Database 7 Hits
1 Chai Li Ping, Su Zhen Zhong, Xian Zhi Xiong Department of Otolaryngology,The First Affiliated Hospital,Sun Yat sen University,Guangzhou,Guangdong, 510080,P.R.China;Inhibition of Hydroxycamptothecin on Laryngeal Squamous Carcinoma Cell Line[J];Chinese Journal of Cancer;2003-04
2 Zhang Yuefei,Chen Yangchao,Zhou Keyuan,Liang TongFrom the Department of Otolaryngology,Affiliated Hospital of Guangdong Medical College,Zhanjiang 524032 (Dr Zhang Yuefei)];bcl-x_s mediated sensitization of human nasopharyngeal carcinoma cells to camptothecin-induced apoptosis[J];;2001-02
3 Kang Mafei,Jiang Hejun,Wang Shikun,et al. Department of Oncology,The Fourth Hospital of Guilin City,Guangxi(Guilin 541001);Effect of hydroxycamptothecin on drug resistant non-small cell lung cancer[J];Guangxi Medical Journal;2003-08
4 ZHANG Yuefei 1 CHEN Yangchao 2 ZHOU Keyuan 2 CAI Kangrong 3 ( 1 Department of Otorhinolaryngology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001; 2Institute of Biochemistry and Molecular Biology, Guangdong Medical College;Mitochondrial mechanisms of CPT-induced apoptosis in nasopharygeal carcinoma cells[J];Journal of Clinical Otorhinolaryngology;2001-07
5 LIN Ying-cheng, WANG Hong-biao, LIN Wen, LIN Yi, LIN Sui-ling, LIN Wen-zhao, ZENG De Department of Medical Oncology, Tumor Hospital Shantou University Medical College,Shantou 515031,P.R.China;Preliminary study of lyophilized 10-Hydroxycamptothecin (Tuoxi) combine with cisplatin in advanced or recurrent malignancies[J];Journal of Qilu Oncology;2005-21
6 ZHANG Yuefei, ZHANG Qun-zhou, CAI Kang-rong et al. (Department of Otorhinolarygology, Affiliated Hospital of Guangdong Medical college, Zhanjiang P.R.China 524023);Influence of several biochemical factors on CPT-induced apoptosis in nasopharyngeal carcinoma cells[J];CHINESE JOURNAL OF LABORATORY DIAGNOSIS;2000-05
7 Li Zhong-han1,Xie Min-qiang2,Chen Shuai-jun2,Wang Lei11Department of Otorhinolaryngology,Head and Neck,Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,Guangdong Province,China; 2Department of Otorhinolaryngology,Head and Neck,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,Guangdong Province,China;Complexing cis-diaminedichloroplatinum-loaded magnetic nano-medicine for treating nasopharyngeal carcinoma in vitro[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2009-29
Chinese Journal Full-text Database 10 Hits
1 ZHAO Bin,GE Jing-fang,LI Jun(School of Pharmacy,Anhui Medical University,Hefei 230032,China);Advances in studies on the anti-tumor mechanisms of camptothecin[J];Anhui Medical and Pharmaceutical Journal;2006-01
2 ZHOU Jun min, CAO Jian guo, LIAO Duan fang Department of Pharmacology, Hengyang Medical College, Hengyang 421001, P R China;Effects of etoposide on growth proliferation and apoptosis in human HIC/S cells[J];CHINESE JOURNAL OF CANCER;2000-04
3 ZHOU Lin, AI Zhong li, LIU Zhi su,et al Department of General Surgery of the Second Affiliated Hospital of Hubei Medical University, Wuhan 430071, P R China;An in vitro study of effect of Kushen compound preparation on human colon cancer cell line SW480[J];CHINESE JOURNAL OF CANCER;2000-04
4 MA Jing1, FU Nai yang2, LI Yu mei3, PENG Wen lie1, XU An long1*1.Department of Biochemistry and Center for Biopharmaceutical Research, College of Life Science, Zhongshan University, Guangzhou 510275, P.R.China2.Institute for Drug Control of Guangdong;Glycyrrhiza uralensis Fisch Extract Selectively Induces Apoptosis in Several Human Cancer Cell Lines in Vitro[J];Chinese Journal of Cancer;2001-08
5 SI Wei ke*, GAO Li hong, LIU Bing, CHENG An, LI Peng, YAO JieDepartment of Clinical Biochemistry, the Third Military Medical University, Chongqing 400038, P.R.China;Regulation of G_1 Phase Cell Cycle Regulation Factors in Differentiation and Apoptosis of Matrine Induced Hepatoma Cells[J];Chinese Journal of Cancer;2001-08
6 YANG Ping, YU Ting xi, MA Run di, YU Li jian*, SU Wei ming, SHAO Hai yan, LIAO Ming neng, HUANG Lai zhen Guangdong Provincial Key Laboratory of Marine Pharmacy,Zhanjiang Ocean University, Zhanjiang 524025,P.R.China;Cell Cycle Arrest and Apoptosis Induced by Tubeimoside in HeLa Cell[J];Chinese Journal of Cancer;2002-04
7 YANG Chun-xu ,HUANG Hai-xing,LI Gui-sheng Department of Oncology,The Fourth Affiliated Hospital,Guangxi Medica l University,Liuzhou 545005,P.R.China;Clinical Study on Patients with Adva nced Gastric Cancer Treated with Oxaliplatin Combining with Hyd roxycamptothecine[J];Chinese Journal of Cancer;2002-08
8 LIU Chengyu, ZHOU Haiyan,WANG Guizhi \ Department of Diagnostics, Qingdao University Medical College, Qingdao 266021\; [;A STUDY ON THE CHANGES OF T LYMPHOCYTE SUBPOPULATION AND IL-2R IN TISSUE AND PERIPHERAL BLOOD OF PSORASIS PATIENTS[J];Acta Acadimae Medicinae Qingdao;2001-01
9 FANG Min, WANG Xiao Dong (Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, USA);The mitochondrial pathways of apoptosis[J];Journal of Beijing Medical University;2002-01
10 ZHANG Dong-yan 1,2 ,ZHAO Xin-qing 1 ,YU Fang 1,BAI Feng-wu 1,AN Li-jia 1 (1.Department of Bioengineering,Dalian University of Technology,Dalian 116012,China; 2.Department of Biology,Liaoning Normal University,Dalian 116029,China);Callus induction of Camptotheca acumianta and camptothecin production[J];Journal of Northeast Normal University (Natural Science Edition);2002-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WANG Shi-liang1,2,CHEN Dian-liang1,DING Man-zhi1,CHU Cheng-ding1,3,YE Hong-yang1,ZHOU Li-chun1,WU Jing1,WANG Jun4(1.Control Release Laboratory,Hefei University of Technology,Hefei 230009;2.Anhui Vocational and Technical College,Hefei 230051;3.Anhui Medical University,Hefei 230032;4.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072);Pharmacokinetics of cisplatin implants in rabbit tongues[J];Anhui Medical and Pharmaceutical Journal;2007-06
2 HUANG He, TAN Wan-long, ZHU Wen-hui, LIANG Zhong-kun Department of Urologic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Lethal effect of adenovirus-mediated HSV-TK gene in combination with hydroxycamptothecin on human bladder cancer in vitro[J];Journal of Southern Medical University;2007-04
3 HE Jie;Nursing Care for Aged Patients with Maxillofacial Squamous Carcinoma Treated by Hydroxycamptothecine and Radiotherapy[J];Journal of Nursing Science;2009-14
4 LI Jin-fang, LIAO Jian-xing (Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Tongji University, Shanghai 200072, China);Telomerase and Telomerase Inhibitor[J];Journal of Oral and Maxillofacial Surgery;2007-03
5 XU Tao,XU Rui-an(Institute of Molecular Medicine,Huaqiao University,Quanzhou 362021,China);Progress on Ati-Tumor Mechanism of Chinese Medicine and Its Active Ingredients[J];Journal of Huaqiao University(Natural Science);2009-04
6 XIANG Hong-lin,GENG Yu-jiao,WU Zhi-juan,ZHAO Xiong-fei,CAO Jian-guo(Medical College of Hunan Normal University,Hunan,Changsha 410006,China);Synthesis and Anti-hepatoma Activities of Apigenin etherified and brominated derivatives[J];Journal of Hunan Normal University(Medical Sciences);2009-03
7 MAO Shengfeng(Zhejiang Forestry College,Lin'an 311300);Study on Antimicrobial Activities of Camptotheca acuminata Leaves Ethanol Extracts to Plant Pathogen[J];Forestry Science & Technology;2009-06
8 AN Yong-qian ,SUN Mo-yi,LI Jian-hu,et al. Department of O ral and Maxillofacial Surgery , Stomatological College,the Fourth Military Medic al University,Xi'an 710032,China.;Experimental study of drug effect on Acc-M cells using MTT colorimetric assay[J];Journal of Modern Oncology;2005-02
9 Lei Delin, Chen Peng, Sun Moyi, et al. College of Stomatology,The Fourth Militar y Medical University, Xi'an 710032,China;Effects of hydroxycamptothecine and pingyangmycin alone or in combination on the proliferation and telomerase activity of human tongue carcinoma cells[J];Journal of Practical Stomatology;2005-01
10 Zhou Bin,Yu ShiyingDepartment of Oncology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030;Radiosensitization Effect of Hydroxycampotothecin on HeLa Cells and Its Influencing Factors[J];Acta Medicinae Universitatis Scientiae et Technologiae Huazhong;2006-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Fu Liwu Pan Qichao Chen Wanshen* Liang YongjuiCancer Institute, Sun Yat-Sen University of Medical Sciences. 510060* South Chinese Plant Research Institute, ACS. 510650;The cytotoxicity of the amnonaceous acetogenins in vitro[J];CHINESE JOURNAL OF CANCER;1997-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved