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《Chinese Journal of Cancer》 2002-05
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Tachyplesin-Induced Differentiat ion of Human Hepatocarcinoma Cell Line SMMC-7721

LI Qi-fu * ,OUYANG Gao-liang,LIU Qing-rong,HONG Shui-gen Laboratory of Cell Biology,The Key L aboratory of Ministry of Education f or Cell Biology &Tumor Cell Engineering,School of Life Sciences,Xiamen Univ ersity,Xiamen 361005,P.R.China  
Background Objective:The study on antitumor activities of marine bioactive substances is an important field in exploiting marin e bioactive substances and antitumo r drugs.And the induction of tumor cell differentiation is a new strategy for drug therapy of t umors.So the authors used tachyplesin,a marine bioactive substance,to investigate its effects on the differentiation of human hepatocarcinoma SMMC-7721cell s for further studying its antitumor activities and mechanisms.Methods:Tachyplesin,which was isolated fro m hemocytes of horseshoe crab(Tachypleus tridentatus),was used to treat human hepatocarcinoma SMMC-7721cells.Light microscopy a nd transmission electron microscop y were applied to examine the changes in morphology and ultrastructure of SMMC-7721cells,respectively.The activ ities of alkaline phosphatase or the expression of AFP and PCNA were assessed by cytochemis try or immunocytochemistry.Results:In the cells treated with 3.0μg /ml tachyplesin,the morphology an d ultrastructure returned to be norm al,the activity of alkaline phosphatase was decreased and the le vels of AFP and PCNA were downregulated.Conclusion:Tachyplesin might effectively reverse the malignant morphology an d ultrastructure,change the activity of enzyme and the levels of a ntigens associated with hepatocarcinoma cell,and induce th e differentiation of the human hepatocarcinoma SMMC-7721cells.
【Fund】: 教育部高等学校骨干教师资助计划项目;; 教育部重点实验室访问学者基金项目
【CateGory Index】: R735.7
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