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《Chinese Journal of Cancer》 2002-08
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Apoptosis of K562Cell Induced by N-p hosphoryl Dipeptide Methyl Ester

NIU Yan-ling,CAO Sheng-li,JIANG Yu-yang ,DU Wei ,ZHAO Yu-fen Key Laboratory of Bioorganic Phosph orus Chemistry,Ministry of Educati on,Department of Chemistry,School of Life Sciences and Engineering,Tsinghua University,Beijing 100084,P.R.China  
Background Objective:Phosphorus plays a crucial role in me tabolism.Some N-phosphoryl amino a cids and N-phosphopeptides have importa nt biological activities and medicinal value.The current study was designed to investigate the apoptosis of K562cells induced b y N-phosphoryl dipeptide methyl ester.Method:Cell growth inhibition of K562cells induced by 15kinds of N-phosphoryl dipeptide methyl esters was analyzed by using MTT assay.The nuclei were stained by Hoechst 33258and the morphologic ch anges were observed.DNA agarose gel electrophoresis,double-staining,and flow cytomery were used to detect early ap optosis of K562cells.Result:(DIPP-L-Leu)2-L-Lys-OCH 3 was the compound screened with MTT method that had better growt h inhibiting activity with the IC 50 of 22.66μmol /L.Chromatin condensation and nuclear fragmentation were seen under fluorescence microscope in the cells treated with Hoechst 33258.DNA a garose gel electrophoresis showed nuclear fra gmentation(DNA ladder).Early apoptotic cells were also detected by flow cytometry.Conclusion:These results suggest that(DIPP-L-Leu) 2 -L-Lys-OCH 3 could induce the apoptosis of K562cells.
【Fund】: 国家自然科学基金项目(No:20132020);; 北京市科委基金;; 清华工研院科技基金
【CateGory Index】: R73-36
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Chinese Journal Full-text Database 2 Hits
1 QIANG Li-ming1,2,LIU Ruo-yu3,2,CAO Shu-xia1,LU Kui2(1.Department of Material and Chemical Engineering,Henan Institute of Engineering,Zhengzhou 450007,China;2.Department of Chemistry,Zhengzhou University,Zhengzhou 450052,China;3.School of Chemistry and Pharmaceutical Engineering,Nanyang Normal University,Nanyang 473061,China);Interaction of N-phosphoryl dipeptide methyl esters and metal ions[J];Journal of Nanyang Normal University;2010-09
2 QIANG Li-ming1,2,YANG Liu1,CAO Shu-xia2,LU Kui1,ZHAO Yu-fen2,3 (1.Department of Material and Chemical Engineering,Henan Institute of Engineering,Zhengzhou 450007,China;2.Henan Province Key Laboratory of Chemical Biology and Organic Chemistry,Department of Chemistry,Zhengzhou University,Zhengzhou 450052,China;3.Educational Ministry Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology,Department of Chemistry,Tsinghua University,Beijing 100084,China);Study on Interaction Between N-phosphoryl Dipeptides (or Methyl Esters) and Amino Acids by ESI-MS[J];Journal of Instrumental Analysis;2009-11
Chinese Journal Full-text Database 10 Hits
1 WANG Cheng_run 1 ,MIN Li 2 ,WU Wei 3 ,et al (1.Department of Chemistry and Biology,Huainan Normal College,Huainan232001,China;2.Library,Huainan Normal College,Huainan2320001,China;3.Biological Engineering Center,Xuzhou Normal University,Xuzhou221116,China);Effects of Holmium Element on DNA Lesion of Mice Liver Cells in Vivo[J];Carcinogenesis,Teratogenesis and Mutagenesis;2004-04
2 XU Jia1,ZHANG Yin-juan2,LU Gang3,SHAN Bao-en3,(1.School of Medicine in Biochemistry,Ji'nan University Guangzhou,510632;2.Clinical Laboratory,People's Hospital of Linfen City,Linfen 041000,Shanxi;3.Research Center,The Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China);Effects of RNAi Targeted to STAT3 on Apoptosis Induction and on STAT3 Signaling in Human Hepatocellular Carcinoma Cells[J];Carcinogenesis,Teratogenesis & Mutagenesis;2009-05
3 SHAO Shu-li et al (College of Life Science and Engineering,Qiqihar University,Qiqihar,Heilongjiang 161006);Effects of Campotothecin(CPT) on Multiplication and Apoptosis of Human Leukemic K562 Cells[J];Journal of Anhui Agricultural Sciences;2010-09
4 ZHU Guo-qi,TANG LI-jian,WANG Lei,SU Jing-jing,WANG Ning,PENG Dai-yin,LI Qing-lin(Anhui College of Traditional Chinese Medicine & Anhui Key Laboratory of Modern Chinese Materia Medica,Anhui Hefei 230038,China);Study on Baicalin Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231[J];Journal of Anhui Traditional Chinese Medical College;2008-02
5 ZHAO Wei hong, CHEN Jia pei, CONG Yu wen,et alInstitute of Radiation Medicine, Beijing 100850,P.R.China;Caffeine promoting tumor cells apoptosis induced by irradiation[J];CHINESE JOURNAL OF CANCER;1999-04
6 Xu Yang1, He Bin2, Wang Ya- Jie1, Fu Qiang1 1. Department of Oncology, Changhai Hospital,The second Military Medical University, Shanghai,200433, P.R.China 2. Department of Cardiothoracic Surgery,Changhai Hospital,The Second Military Medical University, Shanghai,200433, P.R.China;Effect of Herceptin Combined with Doxorubicin on Rat Cardiotoxicity[J];Chinese Journal of Cancer;2004-04
7 LIU Zhao-Yang1,WANG Ze-Guang2,LIU Xing-Jing3,Tian Hai-Mei1,Surapol4,Na Di-Ya2,ZHANG Yi-Fei2,ZHANG Wei1 1.Cancer Institute of Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100021,P.R.China 2.Beijing Clinical Tumour Gene Research Center,Beijing,100022,P.R.China 3.Military Hospital of Guangzhou,Guangzhou,Guangdong,510507,P.R.China 4.Salaya Hospital Thailand;Antitumor Effect and Apoptosis Induction in Human Cancer Cell Lines by BRM-SJS[J];Chinese Journal of Cancer;2004-S1
8 LIU Zhao-Yang1, WANG De-Chang1, FU Zhao-Di2, LIU Hong-Yan2, LIANG Zhi1, CHENG Dong-Wan1, LIANG Jun-Lin1, LIANG Jian-Ming1 1. Cancer Institute, Chinese Academy of Medical Sciences and Peking Union of Medical College, Beijing 100021, P. R. China 2. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union of Medical College, Beijing 100050, P. R. China;Experimental Study on Antitumor Effects of Shen Qi Jin Kang (SQJK)[J];Chinese Journal of Cancer;2006-08
9 LIU Li ping 1, LI Lei 1, ZHANG Ai fang 2, RONG Shi lin 1, LIU Jie 3 (1. Hebei Institute of Physical Education, Shijiazhuang 050041, Hebei, China; 2. Beijing University of Physical Education, Beijing 100084, China; 3. Family Planning Research;Experimental Research on Changes of Blood Biochemical Indexes and Cell Withering during Exercises[J];Journal of Beijing University of Physical Education;2002-03
10 LIU Li ping 1, LI Lei 1, RONG Shi lin 1, ZHANG Ai fang 2, WANG Jian jun 3 (1. Sports Dept., Hebei Institute of Physical Education, Shijiazhuang 050041, Hebei, China; 2. Beijing University of Physical Education, Beijing 100084, China; 3. People's Hospital of Hebei Province, Shijiazhuang 050051, Hebei, China);Experimental Study of Changes of Ca~(2+) and SOD/MDA Ratio in Myocytes and Hepatocytes as well as Apoptosis in Rats during Fatigue[J];Journal of Beijing University of Physical Education;2002-06
China Proceedings of conference Full-text Database 2 Hits
1 ;Effect of Moderate Exercise on Peripheral Blood Oxidative Stress and Lymphocytes Apoptosis in the Psychological Stress Rats[A];[C];2006
2 Wen Zhu~1,Hu Guo-zhu~2,He Dan~2,Yu Huo~1,Rong Ji-ping~1 (1.Jiangxi Institute of Medical Science,Nanchang University,Nanchang 330006,P.R.China;2. Institute of Clinical Medicine of The Jiangxi Province People's Hospital,Nanchang,330006, P.R.China);EFFECT OF LIGUSTRAZINE HYDROCHLORIDE ON PREVENTING FROM THE SUPPRESSION AND APOPTOSIS OF BONE MARROW STROMAL CELLS INDUCED BY VINCRISTINE[A];[C];2009
Chinese Journal Full-text Database 10 Hits
1 LIU Feng1,2, JIANG Yu-Yang2,3, LIU Shi-Ying3,4, WANG Kai1, CAO Jian4, ZHAO Yu-Fen3 1. Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, P. R. China 2. Guangdong Provincial Key Laboratory of Chemical Biology, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong, 518055, P. R. China 3. Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology of Ministry of Education, Department of Chemistry, Tsinghua University, Beijing, 100084, P. R. China 4. Department of Biological Engineering, Henan University of Technology, Zhengzhou, Henan, 450052, P. R. China <;Inducement Effect of (DIPP-L-Trp)2-L-Lys-OCH3 on Apoptosis of K562 Cells Through Mitochondrial-dependent Pathway[J];Chinese Journal of Cancer;2005-04
2 Sheng Li CAO1,2, Yu Yang JIANG1, Yu Ping FENG1, Yan Ling NIU1, Yu Fen ZHAO1* 1Key Laboratory of Bioorganic Phosphorus Chemistry, Ministry of Education; Department of Chemistry, Tsinghua University, Beijing 100084 2Department of Chemistry, Capital Normal University, Beijing 100037;Synthesis and Biological Activities of N-Phosphoryl Branched Peptides[J];中国化学快报(英文版);2003-04
3 CUI Meng, LIU Zhi Qiang, SONG Feng Rui, LIU Shu Ying * (Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China);Gaseous Dipeptide Complexes with Metal Ions by Electrospray Ionization and Tandem Mass Spectrometry[J];Chemical Research In Chinese Universities;2001-07
4 ZOU Xiang-yang~1,LI Lian-hong~2.(~1Department of Biology,Dalian Medical University,Dalian 116027;~2Department of Pathology,Dalian Medical University,Dalian 116027,P.R.China)Correspording anthor: ZOU Xiang-yang.E-mail: zouxiangyang@126.com;Cell Cycle Control and Tumor[J];International Journal of Genetics;2006-01
5 Jiang Bei Lu Zeqin (Dept. of Chem. & Chemical Engineering, Guangxi Normal University Guilin 541004);Structure—Bio-Activity Relation of Organop hosphorus Compound[J];GUANGXI CHEMICAL INDUSTRY;1997-02
6 DU Wei~1, JIANG Yuyang~1, NIU Yanling~1, LIU Feng~2, CAO Shengli~1, MA Li~3, ZHAO Yufen~1Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China; 2. Department of Biological Engineering, Zhengzhou Institute of Technology, Zhengzhou 450052, China; 3. Beijing Biological Medicine Institute, Beijing 100091, China);Apoptosis of K562 cells induced by diisopropylphosphoryl leucyl-lysine methyl ester[J];Journal of Tsinghua University(Science and Technology);2003-12
7 Chen Jiayu, Tang Qong, Zhu Line, Liu Yubing and Yang Huarong (Dept. of Pharmacology, West China University of Medical Sciences, Chengdu 610041);The Anti-inflamatory and Analgesic Effect of Jingengteng Tablet[J];Sichuan Journal of Physiological Sciences;2000-01
8 Chao Qi, Liu Xingjie; Zhang Deoheng(Department of Chemistry of Naturat Drugs, School of Pharmacy, Shanghai Medical University, Shanghai);STUDIES ON THE CHEMICAL CONSTITUENTS OF SMILAX CHINA[J];;1989-03
9 Kong Jianqiang Zhao Qi (Biology Department in the Capital Normal University,Beijing 100037);Advance in Mechanism of Apoptosis[J];Biotechnology Information;2002-03
10 WU Jian-li~1,CHEN Xiao-lan~1,QU Ling-bo~(1,2),LU Jian-sha~1,ZHAO Yu-fen~(1,3)(1.Department of Chemistry,Key Laboratory of Chemistry and Biology and Organic Chemisty,Zhengzhou University,Zhengzhou 450052,China;2.Anyang Normal College,Anyang 455002,China;3.Key Laboratory forBioorganic Phosphorus Chemistry,Ministry of Education,Department of Chemistry,School of LifeSciences and Engineering,Tsinghua University,Beijing 100084,China);Study on the Interaction of α-Lactalbumin and Dithyl Flavon-7-yl Phosphate by ESI Mass Spectrometry[J];Journal of Instrumental Analysis;2006-03
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 LI Ming-ze,TIAN Song,YU Lei,LANG Lang,ZOU Xiang,JI Yu-bin (Center of Reserch and Development on Life Science and Environmental Sciences, Harbin University of Commerce,Harbin 150076,China);Study on antitumor activities of isohumulone in Humulus Lupulus L. in vitro and its mechanism[J];Journal of Harbin University of Commerce(Natural Sciences Edition);2007-01
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