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《Chinese Journal of Cancer》 2002-08
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Apoptosis of K562Cell Induced by N-p hosphoryl Dipeptide Methyl Ester

NIU Yan-ling,CAO Sheng-li,JIANG Yu-yang ,DU Wei ,ZHAO Yu-fen Key Laboratory of Bioorganic Phosph orus Chemistry,Ministry of Educati on,Department of Chemistry,School of Life Sciences and Engineering,Tsinghua University,Beijing 100084,P.R.China  
Background Objective:Phosphorus plays a crucial role in me tabolism.Some N-phosphoryl amino a cids and N-phosphopeptides have importa nt biological activities and medicinal value.The current study was designed to investigate the apoptosis of K562cells induced b y N-phosphoryl dipeptide methyl ester.Method:Cell growth inhibition of K562cells induced by 15kinds of N-phosphoryl dipeptide methyl esters was analyzed by using MTT assay.The nuclei were stained by Hoechst 33258and the morphologic ch anges were observed.DNA agarose gel electrophoresis,double-staining,and flow cytomery were used to detect early ap optosis of K562cells.Result:(DIPP-L-Leu)2-L-Lys-OCH 3 was the compound screened with MTT method that had better growt h inhibiting activity with the IC 50 of 22.66μmol /L.Chromatin condensation and nuclear fragmentation were seen under fluorescence microscope in the cells treated with Hoechst 33258.DNA a garose gel electrophoresis showed nuclear fra gmentation(DNA ladder).Early apoptotic cells were also detected by flow cytometry.Conclusion:These results suggest that(DIPP-L-Leu) 2 -L-Lys-OCH 3 could induce the apoptosis of K562cells.
【Fund】: 国家自然科学基金项目(No:20132020);; 北京市科委基金;; 清华工研院科技基金
【CateGory Index】: R73-36
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