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《Chinese Journal of Cancer》 2002-08
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Effect of Boanmycin on Apoptosis and Cell Cycle of Human Esophageal Cancer(Eca-109)Cells

TANG Hao 1 ,YANG Xiao-ping 2 1.Department of Internal Medicine,Hubei Cancer Hospital,Wuhan 430079,P.R.China;2.Cancer Institute,Cancer Center,Sun Yat-sen University,Guangzhou 510060,P.R.China  
Background Objective:The anticancer mechanism of boanmyc in was thought to inhibit the synthesis of protein in human esophageal cancer c ell line.But it is not clear that there are other mechanisms of boanmycin t o produce its anticancer effect on human esophage al cancer.This study was designed to examine the effect of boanmycin on ap optosis and cell cycle of human esophageal cancer cells(Eca-109).Methods:Microscopic observation,flow cyto metry,and agarose gel electrophoresis were used to determ ine the induction of apoptosis and th e changes of cell cycle.Results:The human esophageal cancer xenografts in nude mice treated by boanmycin showed mo rphological changes of different ap optotic phases.In vitro,boanmycin(50-125μg /ml,30-36hours)elicited typical apoptotic changes in Eca-109cells and typical DNA ladder of apoptotic cells appeared.The Eca-109cells treated by boanmyc in for 42hours were blocked at G 2 /M phase.Conclusion:Boanmycin could induce apoptosis of Eca-109cells and block the cell cycle at G 2 /M phase.
【CateGory Index】: R735.1
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