Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cancer》 2002-09
Add to Favorite Get Latest Update

Apoptosis of Human Lung Carcinoma Ce ll Line GLC-82Induced by High Power Electromagnetic Pulse

CAO Xiao-zhe * ,ZHAO Mei-lan,WANG De-wen,Dong Bo Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,P.R.China  
Background Objective:Electromagnetic pulse(EMP )could be used for sterilization of fo od and the efficiency is higher than 2450MHz co ntinuous microwave done.This study was designed to evaluate the effect o f electromagnetic pulse(EMP )on apoptosis of human lung carcinoma cell line GLC-82,so that to explore a nd develop therapeutic means for cancer.Methods:The injury changes in GLC-82cells after irradiated with EMP (electric field intensity was 60kV /m,5pulses /2min)were analyzed by cytometry,MTT chro nometry,and flow cytometry.The immunohistochemical SP staining wa s used to determine the expressions o f bcl-2protein and p53protein.The s tained positive cells were analyzed by CMIA S-II image analysis system at a magni fication 400.All data were analyzed by SPSS8.0software.Results:EMP could obviously inhibited proliferation and activity of lung carcin oma cell line GLC-82.The absorbance value(A570)of MTT decreased immediately,at 0h,1h,and 6h after the GLC-82cells irra diated by EMP as compared with control group.The hig hest apoptosis rate was found to reac h 13.38%by flow cytometry at 6h after EMP irradiation.Down-regulation of bcl-2expression and up-regulation of p53expression were induced by EMP.Conclusion:EMP promotes apoptosis of GLC-82cells.At same time,EMP can down-regula te bcl-2expression and up-regulate p53expression in GLC-82cells.The bcl-2and the p53protein may involve the apoptotic process.
【Fund】: 解放军总后勤部“十五”指令课题基金资助(No.01L023)
【CateGory Index】: R734.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 7 Hits
1 CAO Xiao zhe,WANG De wen,ZHAO Mei lan,CUI Xue mei (Institute of Radiation Medicine, Academy of Military Medical Sciences,Beijing 100850,China);Study on the ultrastructural changes of the adrenal gland in rats irradiated by electromagnetic pulse(EMP)[J];Journal of Chinese Electron Microscopy Society;2002-01
2 ZHAO Mei lan 1, CAO Xiao zhe 1,WANG De wen 1, LI Chuan lu 2, LIU Cun hua 2,PENG Guang shun 2,TAN Qi mei 2 (1 Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China; 2 College of Science, National U;High Field Strength EMP with Its Applied Study in Biology[J];Journal of National University of Defense Technology;2000-S1
3 CAO Xiao_Zhe 1, WANG De_Wen 1, ZHAO Mei_Lan 1, GAO Ya_Bing 1, PENG Ruei_Yun 1, CUI Xue_Mei 1, CHEN Hao_Yu 1, CHEN Jian_Kui 2 (1.Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850,China;2.Affiliated Hospital,Aca;Serum biochemical indexes in macaques irradiated by electromagnetic pulse[J];Bulletin of The Academy of Military Medical Sciences;2002-01
4 Liu Changjun 1 Wang Baoyi 1 Zhang Hong 2 Li Fengqun 2 Zou Dahua 2 1(Department of Radio Electronics Sichuan Union University, Chengdu 610064) 2(Institute of Electronics Technology, Sichuan Union University, Chengdu 610064);Mechanism of Specific Effects of Transient Electromagnetic Pulses on Cell Model[J];JOURNAL OF BIOMEDICAL ENGINEERING;1998-03
5 ZHOU Weihong,ZHANG Jun(Lab 408,National University of Defence Technology,Changsha 410 073);Study on the Disinfection Technology of Electromagnetic Pulse[J];JOURNAL OF MICROWARES;2000-03
6 ZHANG Hong Feng CHEN Shu De WANG Yao Fa CHEN Jia Sen (Biology Department , Physics Department, East China Normal University);THE EFFECTS OF PULSED ELECTRIC FIELD ON DERMAL FIBROBLAST PROLIFERATION AND MEMBRANE FLUIDITY[J];Chinese Journal of Coll Biology;1998-02
7 CUI Yufang , YANG Hong , GAO Yabing , CUI Xuemei , XIONG Chengqi GAO Linlu and WANG Dewen Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China (Cui YF, Yang H, Gao YB, Cui XM, Xiong CQ, Gao LL and Wang DW);Effect and mechanism of electronic magnetic pulse on peripheral lymphocytes in dogs[J];中华医学杂志(英文版);2001-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 GUO Qing gong, MA Xue lian (College of Communication and Information Engineering, CUPT, Chongqing. 400065, China);Essential Factors Influencing Experimental Repetition of the Transient Electromagnetic Biological Effects[J];Journal of Chongqing University of Posts and Telecommunications;2002-01
2 GUO Qing-gong 1, MA Xue-lian 1, ZHANG Hong 2, WANG Bao-yi 2 (1.College of Telecommunication Enginering, CUPT, Chongqing 400065,China; 2. College of Electronic Information, Sichuan University, Chengdu 610064,China);Effect of Electromagnetic Pluse Radiation on Cancer Cells[J];Journal of Chongqing University of Posts and Telecommunications;2002-02
3 GUO Qing gong 1,MA Xue lian 1, ZHANG Hong 2 (1. College of Communications and Information Engineering, CUPT, Chongqing 400065,P.R.China; 2. Department of Radio Electronics, Sichuan University, Chengdu 610064,P.R.China);The Effects of Weak Transcient Electromagnetic Pulses on Heredity and Immunity of the Lymphocytes[J];Journal of Chongqing University of Posts and Telecommunications;2002-04
4 YAO Xueling,CHEN Jingliang(Xian Jiao Tong University,Xian 710049,China);An Approach to Electromagnetic Protection Distances in Pulse High Current Dischanging Environment[J];Insulators and Surge Arresters;2002-06
5 Bei Weihui Cao Yi(School of Radiation Medicine and Public Health,Suzhou University,Suzhou,215123);Biological Effects of Electromagnetic Radiation[J];Radiation Protection Bulletin;2007-03
6 YAO Xue\|ling, CHEN Jing\|liang, XU Chuan\|xiang(Xian Jiaotong University, Xian 710049, China);Analysis for non-thermal effects of pulsed current electromagnetic fields on biological membranes[J];Advanced Technology of Electrical Engineering and Energy;2003-02
7 WANG Jing, HU Yu-cai, LI Li(School of Science, Dalian Fisheries University, Dalian 116023, China);Comparative analysis of non-thermal electromagnetic biological effects and electrohydrodynamic instability in liquid crystals[J];Journal of Dalian Fisheries University;2003-02
8 LI Jing; WU Yan-Zhuo;UO Yao; (Department of Radiation Medicine, Faculty of Military Health Service & Statistics, Fourth MilitaryMedical University Xi'an 710033, China,) ZHENG Zhen-Xing; (Institute of Northwest Nuclear Technology);Effect of double NEMP on the learning and memory ability of rats[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-01
9 REN Dong Qing 1, GUO Yao 1, GUO Guo Zhen 1, ZENG Gui Ying 1, ZHAO Tao 1, ZHENG Zhen Xing 2 1Department of Radiomedicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710033, China, 2Northwest Institute of Nuclear Technology, Xi'an 710024, China;Different effects of electromagnetic pulse and ionizing radiation on survival rate of human intestinal epithelial cells[J];Journal of The Fourth Military Medical University;2000-03
10 QU Run Jiang 1, GUO Yao 1, WANG Shu Fang 2, ZHENG Zhen Xing 1 1Department of Radiation Medicine, Faculty of Preventive Medicine,Xi'an 710033, China, 2Department of pathology, Tangdu Hospital, Xi'an 710038,China,Fourth Military Medical Unive;Effects of electromagnetic pulse on Fos protein in hypothalamic suprachiasmatic nucleus and pineal glands of rats[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-08
China Proceedings of conference Full-text Database 2 Hits
1 CAO Xiaozhe1 , ZHAO Meilan2, WANG Dewen2 (1. Department of pathology, The 2nd Pepper’s Hospital of Zhuhai, Zhuhai 519020, Guangdong Province, China; 2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850);The Atomic Force Microscopy Study on The Hypothalamus Neurons Membrane Perforate Induced by EMP[A];[C];2008
2 WangBaoyi, Zhang Hong, Chen Haichuan , Wang Zishu, Yang Kong, Sun Jingru (College of Electron-information,Sichuan University.Chengdu 610064) (College of Life Science,Sichuan University.Chengdu 610064);An experiment studies to the treatment of sarcomas by the electroporation therapy[A];[C];2002
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Ma Zeng-chen Tang Zhao-you, Yu Ye-qin, Lin Zhi yingLiver Cancer Research Unit, Zhongshan Hospital, Shanghai First Medical College, Shanghai;STUDIES IN EXPERIMENTAL HEPATOMA MODELS FROM CLINICAL VIEW POINT I. COMPARISONS OF RAT HEPATOMA MODELS AND PRELIMINARY OBSERVATION OF TRANSPLANTA-BLE HUMAN HEPATOMASIIN NUDE MICE[J];Chinese Journal of Cancer;1983-04
2 PENG Xiao-ping1,LI Jun-dong1,LI Meng-da1*,YE Xian-ming2,YAN Wan-chang2 1.Department of Gynaecology,Cancer center,Sun Yat-sun University,Guangzhou 510060,P.R.China2.Department of Obstetrics and Gyna ecology,Queen Mary Hospital,Hong Kong University,Hong;Clinical Significance of Vascular Endothelial Growth Factor in Sera of Patients with Gynaecological Malignant Tumors[J];Chinese Journal of Cancer;2002-02
3 Zhai Bao-Jin1, Shao Ze-Yong1, Wu Feng1, W ang Zhi-Biao2 1.The Center for Tumor Therapy,The 2nd Affiliated Hospital,Chongqing University of Medical Science, Chongqing,400016, P.R.China 2.Institute of Ultrasound and Engineering in Medicine,Chongqing University of Medical Science, Chongqing,400016, P.R.China;Reversal of Multidrug Resistance of Human Hepatocarcinoma HepG2/Adm Cells by High Intensity Focused Ultrasound[J];Chinese Journal of Cancer;2003-12
4 ZHAI Bao Jin, WU Feng, SHAO Ze Yong, HU Kai, ZHAO Chun Liang, WANG Zhi Biao Department of Biomedical Engineering, Institute of Ultrasound and Engineering in Medicine, Chongqing University of Medical Science, Chongqing,400016, P.R.China;Establishment of In Vivo Adriamycin induced Multidrug Resistance Models of Subcutaneous and Hepatic Transplanted Human Liver Cancer in Nude Mice[J];Chinese Journal of Cancer;2004-08
5 YANG Lu jun 1, Nuesse Michael 2, AO Lin 1, CAO Jia 1 (Department of Hygiene Toxicology, Third Military Medical University, Chongqing 400038, China; 2GSF National Research Center for Environment and Health, Neuherberg, Germany,D 85764);Tripterygium Hypoglaucum (Levl) Hutch alkaloids induce DNA strand breaks and DNA fragmentation in Jurkat T lymphoma cells[J];Acta Academiae Medicinae Militaris Tertiae;2003-17
6 REN Dong Qing 1, GUO Yao 1, GUO Guo Zhen 1, ZENG Gui Ying 1, ZHAO Tao 1, ZHENG Zhen Xing 2 1Department of Radiomedicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710033, China, 2Northwest Institute of Nuclear Technology, Xi'an 710024, China;Different effects of electromagnetic pulse and ionizing radiation on survival rate of human intestinal epithelial cells[J];Journal of The Fourth Military Medical University;2000-03
7 ZHANG Hong Xin 1,GUO Wei Ping 1,WANG Zhi Min 1,WANG Yi Qing 1,LIU Yan 2,GAO Wei 3,LI Wen Xian 1,NI Dai Hui 1,LIANG Zhi Hui 1,CAO Wei 1,GUAN Yan 1 1Department of Interventional Radiology, 2Departm ent of Ophthalmology, 3D;Comparative study of the effect of thermochemothr-rapy of ADM on human multidrug-resistant hepatocellular carcinoma cell line (7721/Adm)[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-08
8 WU Yu 1, JIANG Nan Yan 2, CHENG Qing Shu 1, LI Xiao Fei 1, WANG Xiao Pin 1, LIU Kun 1 1Department of Thoracic Surgery, 2Clinical Laboratory, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China;Assessment of immune cells from patients with lung cancer before and after radiofrequency ablation[J];Journal of The Fourth Military Medical University;2004-07
9 PAN Yao qian,GAO Feng,CHENG Jun (Faculty of Animal Medicine Quartermaster University of PLA,Changchun 130062,China);Research Rrogress between Apoptosis and Cellular Nectosis[J];Progress In Veterinary Medicine;2000-04
10 WU Yanglan, Wang YUANliang of Education, Chongqing University, Chongqing 400044,China);Tumor Neoangiogenesis Inhibitors——Angiostatin and Endostatin Implication[J];Journal of Chongqing University(Natural Science Edition);2003-10
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 Li Chengxiang1 Yao Chenguo1 Mi Yan1 Xiong Zheng'ai21 (State Key Laboratory of Power Transmission Equipment & System Security and New Technology,Chongqing University,Chongqing 400044,China)2 (Second Affiliated Hospital,Chongqing Medical Science University,Chongqing 400061,China);Recent Advances on Physical Ablation for Tumor[J];Journal of Biomedical Engineering;2009-05
2 XIONG Lan~1 SUN Cai-Xin~1 WANG Shi-Bin~(2*) YAO Chen-Guo~1 MI Yan~1 YANG Xiao-Jun~3 HU Li-Na~3()~1(Key Lab of High Voltage Engineering and Electrical New Technology,Ministration of Education,Chongqing University,Chongqing 400044) ()~2(Chongqing Institute of Technology,Chongqing 400050)()~3(Second Affiliated Hospital,Chongqing University of Medical Science,Chongqing 400061);Steep Pulsed Electric Field Simulation and Rabbit Liver Tissue in vivo Experiment[J];Chinese Journal of Biomedical Engineering;2007-02
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Wang Changqing;Zhu Xili(Peking University,Beijing 100871);Research on Interaction of Pulse Electromagnetic wave With Human Body[J];ACTA ELECTRONICA SINICA;1994-06
2 ZHAO Mei lan 1, CAO Xiao zhe 1,WANG De wen 1, LI Chuan lu 2, LIU Cun hua 2,PENG Guang shun 2,TAN Qi mei 2 (1 Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China; 2 College of Science, National U;High Field Strength EMP with Its Applied Study in Biology[J];Journal of National University of Defense Technology;2000-S1
3 ZHAO MeiLan, CAO XiaoZhe, CUI XueMei, WANG DeWen(Institute of Radiation Medicine,Academy of Military Medical Scienc es, Beijing 100850 ,China);Influence of EMP on the C-FOS expression in hippocampus[J];Bulletin of The Academy of Military Medical Sciences;2001-03
4 Zhao Nan-ming (Institute of Modern Biology and Biomedical Engineering, Tsinghua University) Poo Mu-ming (Department of Physiology and Biophysics,University of California, Irvine, CA 92717, USA);The Theory in Situ Electrophoresis of Cell Membrane Components[J];Journal of Tsinghua University(Science and Technology);1983-04
5 Liu Changjun Wang Baoyi(Department of Radio Electronics,Sichuan University)Zhang Hong(Chengdu Institute of Electronics Technology);ENERGY CALCULATION AND ANALYSIS OF AN ION CROSSING MEMBRANE[J];JOURNAL OF SICHUAN UNIVERSITY (NATURAL SCIENCE EDITION);1998-01
6 Liu Changjun 1 Wang Baoyi 1 Zhang Hong 2 Li Fengqun 2 Zou Dahua 2 1(Department of Radio Electronics Sichuan Union University, Chengdu 610064) 2(Institute of Electronics Technology, Sichuan Union University, Chengdu 610064);Mechanism of Specific Effects of Transient Electromagnetic Pulses on Cell Model[J];JOURNAL OF BIOMEDICAL ENGINEERING;1998-03
7 ZHOU Weihong,ZHANG Jun(Lab 408,National University of Defence Technology,Changsha 410 073);Study on the Disinfection Technology of Electromagnetic Pulse[J];JOURNAL OF MICROWARES;2000-03
8 CAO Xiao zhe,XING Chuan ping,YANG Sheng yue, et al .(Lanzhou General Hospital,Lanzhou Command,PLA,730050);Electron microscopic study on the influence of simulated high altitude environment on ultrastructures of diaphragm in rats[J];MEDICAL JOURNAL OF NATIONAL DEFENDING FORCES IN NORTHWEST CHINA;2000-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved