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《Chinese Journal of Cancer》 2003-03
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Apoptosis of Human Gastric Cancer Cell Induced by Photochemical Riboflavin

Li Jin Quan1,Chen Rui Chuan2,Cai Ke Xia2,Ye Zhi Yun11.Department of Biology,2. Cancer Research Center,Life Science School of Xiamen University, Xiamen, Fujian, 361005, P.R.China  
BACKGROUND OBJECTIVE: Photochemical riboflavin, the production of reactive oxygen species (ROS) , has been reported having cytotoxity on some cancer cells. The aim of this study was to investigate the effect of photochemical riboflavin on inducing apoptosis in human gastric cancer cell line MGC80 3. METHODS: Trypan blue exclusion method, Giemsa staining, DNA electrophoresis, DNA quantification, Western blot analysis, and flow cytometry were conducted to determine the effect of photochemical riboflavin on cell survival, morphology, DNA fragmentation, gene expression, and cell cycle arresting. RESULTS: The cell viability dropped down according to the riboflavin concentration and treating time. Exposure of the cells in 20 μmol/L riboflavin for 24 hours resulted in typical apoptotic morphology and G2/M arresting. As MGC80 3 cells were separately treated with 10, 20, 30, and 40 μmol/L photochemical riboflavin, DNA electrophoresis showed that the ladder bands, a typical feature of apoptotic cell, appeared in the groups treated by over 20 μmol/L photochemical riboflavin. The efficiency rates of DNA fragmentation were 35.4%, 54.1%, 70.6%, and 86.8%, respectively. Western blot analysis showed that the expression of apoptosis related proteins p53, C myc, and Bax were up regulated whereas the expression of Bcl 2 was down regulated. CONCLUSION: These results indicate that photochemical riboflavin has high efficiency of inducing cell apoptosis on MGC 80 3 cells in vitro and there is a correlation between apoptosis and G2/M arresting.
【Fund】: 福建省自然科学基金项目(C97108)
【CateGory Index】: R735.2
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