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《Chinese Journal of Cancer》 2003-03
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Effects of Lovastatin on Proliferation and Gap Junctional IntercellularCommunication of Human Breast Cancer Cell MCF-7

Zhou Yong, Mi Man Tian, Zhu Jun Dong, Zhang Qian YongDepartment of Nutrition and Food Hygiene,The Third Military Medical University,Chongqing,400038,P.R.China  
BACKGROUND OBJECTIVE:Lovastatin,an inhibitor of endogenous cholesterol biosynthesis,has been widely used in the clinical treatment of hypercholesterolemia.Recently,lovastatin has been paid more attention for its wide range effects on human cancer cells; however,the detail mechanisms of its anti cancer effects are not yet understood. This study was designed to investigate the effects of lovastatin on proliferation and gap junctional intercellular communication (GJIC) of MCF 7 human breast cancer cells. METHODS:After treated with lovastatin at dosages of 4,8,16 μmol/L for 1-3 days,the cell differentiation was examined with nitroblue tetrazolium (NBT) reduction test;the proliferation and distribution of cell cycles were examined with flow cytometry (FCM). Meanwhile,GJIC of MCF 7 cells was observed using the scrape loading and dye transfer(SLDT) technique. RESULTS:Lovastatin could inhibit the proliferation of MCF 7 cells significantly and 75.80 percent of cells were inhibited after treated with 16 μmol/L lovastatin for 72 hours (P 0.05). Meanwhile, lovastatin could arrest MCF 7 cells in the G0/G1 phase of cell cycle and 80 percent of cells were arrested in G0/G1 phase after treated with lovastatin for 72 hours. Furthermore, lovastatin could induce the differentiation of MCF 7 cells (P 0.01) and up regulate GJIC in MCF 7 cells. After treated with 16 μmol/L lovastatin for 72 hours, transfer of LY fluorescence could reach 4-5 rows of cells from the scraped line. However, apoptosis in MCF 7 cells was not obvious. All these effects of lovastatin were in a dose and time dependent manner. CONCLUSION:It suggests that lovastatin has the capabilities of inhibiting proliferation, arresting MCF 7 cells at G0/G1 phase of cell cycle and inducing differentiation. These effects of lovastatin maybe correlate with lovastatin promoting GJIC function in MCF 7 cells.
【Fund】: 国家自然科学基金项目(No.30271128)
【CateGory Index】: R73-36
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