Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cancer》 2004-05
Add to Favorite Get Latest Update

Effect of Epidermal Growth Factor Receptor-selective Tyrosine Kinase Inhibitor ZD1839 on Nasopharyngeal Carcinoma Cells

Wang Shu Sen, Guan Zhong Zhen, Jiang Wen Qi, Lin Tong Yu, Zhang Li Department of Internal Medicine, Cancer Center,Sun Yat sen University, Guangzhou, Guangdong,510060, P.R.China  
BACKGROUND OBJECTIVE: ZD1839, an anilinoquinazoline, is an orally active, selective epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor, which has been approved for the treatment of advanced non small cell lung cancer by FDA. Nasopharyngeal carcinoma is of epithelial origin with overexpression of epidermal growth factor receptor and it remains unclear whether ZD1839 is useful in the treatment of nasopharyngeal carcinoma. The present study was designed to evaluate the effect of ZD1839 on nasopharyngeal carcinoma cell lines. METHODS: The effect of ZD1839 on nasopharyngeal carcinoma cells (CNE1, CNE2, SUNE1) was determined by MTT assay. The effect of ZD1839 combined with cisplatin (DDP) or 5 fluouracil (5 FU) on nasopharyngeal carcinoma cell CNE2 was also evaluated. Bürgi formula was adopted to analyze combination effect. The distribution of cell cycle and cell apoptosis rate were analyzed by flow cytometry. RESULTS: The proliferation of nasopharyngeal carcinoma cell lines CNE1, CNE2, and SUNE1 was inhibited by ZD1839, and the effect was dose dependent. The value of IC50 for the effect of ZD1839 on nasopharyngeal carcinoma cell lines CNE1, CNE2, and SUNE1 was 3.9 μmol/L, 5.6 μmol/L, and 5.5 μmol/L, respectively. ZD1839 could enhance the effect of DDP and 5 FU on CNE2 cells. The Q values by Bürgi formula were 1.19±0.02 and 1.12±0.10, respectively. When CNE2 cells were treated with ZD1839 at the concentration of 0, 1.95, 3.9, 7.8, 15.6, 31.25 μmol/L, the percentages of G0/G1 phase were (46.8±1.7)%, (48.8±1.6)%, (51.3±1.3)%, (54.0±1.3)%, (61.5±2.2)%, and (71.2±1.4)%, respectively; the percentages of S phase were (37.5±1.3)%, (35.8±1.6)%, (31.8±2.1)%, (34.3±2.7)%, (27.2±2.9)%, and (27.6±2.4)%, respectively; the percentages of G2/M phase were (15.7±0.4)%, (15.3±0.1)%, (16.9±0.9)%, (11.7±1.4)%, (11.3±0.7)%, and (1.1±0.8)%, respectively. When CNE2 cells were treated with ZD1839 at the concentration of 0, 1.95, 3.9, 7.8, 15.6, 31.25, 62.5 μmol/L, the rates of CNE2 cell apoptosis were (1.6±0.3)%, (1.7±0.3)%, (2.3±0.4)%, (3.3±0.4)%, (3.9±0.8)%,(8.0±1.1)%,and(14.3±2.3)%, respectively. CONCLUSION: ZD1839 inhibits the proliferation of nasopharyngeal carcinoma cells and enhances the effect of cytotoxic drugs. ZD1839 induces CNE2 cell cycle arrest in the G1 phase and higher concentration of ZD1839 could induce apoptosis.
【CateGory Index】: R739.63
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Shu-Sen,GUAN Zhong-Zhen,XIANG Yan-Qun,JIANG Wen-Qi,LIN Tong-Yu,ZHANG Li Department of Medical Oncology,Cancer Center,Sun Yat-sen University,Guangzhou,Guangdong,510060,P.R.China;Effect of Epidermal Growth Factor Receptor-selective Tyrosine Kinase Inhibitor Gefitinib on Nasopharyngeal Carcinoma Xenografts[J];Chinese Journal of Cancer;2004-S1
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Chen Xiaoyin,Shen Zhuoqun (Departmant of TCM,Medical College of Jin'an University,Guangzhou 510632,China);EFFECTS OF QIONGYUGAO ON THE EXPRESSION OF NM23 AND PCNA OF EXPERIMENTAL LUNG CANCER MICE TREATED BY CHEMOTHERAPY[J];JOURNAL OF ANHUI TRADITIONAL CHINESE MEDICAL COLLEGE;2000-02
2 GUAN Zhong 1, YE Hui 1, YANG Xiao ping 2,et al.1.Sun Yat sen Memorial Hospital, Sun Yat sen University of Medical Sciences, Guangzhou 510120,P.R.China. 2.Cancer Institute, Sun Yat sen University of Medical Sciences,Guangzhou 510080,P.R.China.;Boanmycin induces apoptosis in human nasopharyngeal cancer cells[J];CHINESE JOURNAL OF CANCER;2000-04
3 LIANG Tong, CHEN Mei-Jun, ZHOU Ke-Yuan, TANG Xu-Dong, WANG Xu-Guang Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang,Guangdong,524023, P.R.China;Induction of Apoptosis in Human Nasopharyngeal Carcinoma Cell Line CNE-2Z by Curcumin[J];Chinese Journal of Cancer;2004-12
4 CHENG Chao1, WU Yi-Long2, GU Li-Jia1, CHEN Gang2, WENG Yi-Min1, FENG Wei-Neng1, ZHONG Wen-Zhao1 1. Department of Thoracic Surgery, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510630, P. R. China 2. Guangdong Provincial Institute of Lung Cancer Research, Tumor Center, Guangdong Provincial People's Hospital, Guangzhou, Guangdong, 510080, P.R.China;Predicting Efficacy of Neoadjuvant Cheomotherapy on Resectable Stage ⅢA Non-small Cell Lung Cancer by Multi-gene Expressions[J];Chinese Journal of Cancer;2005-07
5 ZHU Zhi-Hua1, RONG Tie-Hua1, ZENG Can-Guang1, WU Qiu-Liang2, MA Yun3, HUANG Xiao-Ping1, LI Bao-Jiang1, ZHANG Peng-Yuan1, ZHAO Jin-Ming1, HU Wei1, ZHANG Shi-Yi1, YU Hui1, MA Guo-Wei1, ZHANG Lan-Jun1, WEN Zhe-Sheng1, FU Jian-Hua1, LONG Hao1 1. Department of Thoracic Surgery, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P.R.China 2. Department of Pathology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P.R.China 3. Center of Experimental Animal, Sun Yat-sen University, Guangzhou, Guangdong, 510080, P.R.China;Vascular Endothelial Growth Factor Expression and Microvessel Density in StageⅠ-Ⅱ Non-small Cell Lung Cancer and Their Prognostic Significances[J];Chinese Journal of Cancer;2005-07
6 YUAN Tai-Ze1,2, LI Xiao-Xia1,3, CAO Yun1,3, QIAN Chao-Nan1,2, ZENG Mu-Sheng1,4, GUO Xiang1,2 1. State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510060, P. R. China 2. Department of Nasopharyngeal Carcinoma, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P. R. China 3. Department of Pathology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P. R. China 4. Research Department, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P. R. China;Correlation of Epidermal Growth Factor Receptor Activation to Metastasis-free Survival of Nasopharyngeal Carcinoma Patients[J];Chinese Journal of Cancer;2008-05
7 XIE Bingfen; PAN Qichao; LIU Zongchao; PAN Weiguang; LIANG Yongju; BI Yisun (Departmentof Therapeutic Basis,Cancer Institute, SUN Yet-sen University of Medical Science,Guangzhou 510060);ENHANCED ANTITUMOR EFFECT OF ANTICANCER DRUGS BY GUBENYILIU PRESCRIPTION NoⅡ(GUB)[J];CHINESE JOURNAL OF CANCER;1996-03
8 Chen Yamin Liu Jiwei Zhao Yi Department of Oncology,the First Affiliated Hospital of Dalian Medical University,Dalian 116011,China;Clinical observation of gemcitabine plus cisplatine in treatment advanced of non-small cell lung cancer[J];Oncology Progress;2004-04
9 Li Tao1 Song Cuiping2 1 CAMS & PUMC,Beijing 100730,China 2 Beidaihe Hospital,Qinhuangdao,Hebei Prouince,Qinhuangdao 066100,China;Comparable observation of toxicity and clinical activity by navelbine plus cisplation,gemcitabine plus cisplation the raping nonsmall cell lung cancer[J];Oncology Progress;2007-02
10 Xu Xuejun, Zhou Zicheng, Luo Yuanhui, Lu Rong, Men Rongfu, Fang Dianchun (Department of Gasenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038);Elemene induced apoptosis of SMMC 7721 cells of human hepatocellular carcinoma[J];ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE;1999-04
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 SONG Xin, LI Li, MA Xiao-biao, et al.;Progress in Antibody and Small Molecular Compound in Treatment for Nasopharyngeal Carcinoma[J];Journal of Oncology;2008-01
2 PAN Jian-ji,LIN Se-nan,ZONG Jing-feng (Department of Radiation Oncology,Fujian Provincial Cancer Hospital Affiliated to Fujian Medical University,Fuzhou Fujian 350014,China);Study progress of tumor marker related to nasopharygeal carcinoma[J];China Oncology;2008-09
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved