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《Chinese Journal of Cancer》 2004-08
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Induction of Differentiation by Ginsenoside Rh_2 in Hepatocarcinoma Cell SMMC-7721

ZENG Xiao Li1, TU Zhi Guang2 1.Clinical Laboratory of Anzhen Hospital,Capital University of Medical Sciences, Beijing,100029, P.R.China 2.Department of Clinical Biochemistry,Chongqing University of Medical Sciences, Chongqing,400016, P.R.China  
BACKGROUND OBJECTIVE: Up to now, searching for non toxic and natural origin substances that induced the differentiation of cancer cells is a key for anticancer therapy. Ginseng is one of the most widely used natural tonics in oriental countries for thousands of years and has been reported to have various biological effects. Ginsenosides are thought to be the major effective ingredients in ginseng. Among them, ginsenoside Rh2(G Rh2) has been suggested to have a cell growth suppressive effect on various cancer cells, but the mechanism is unclear.This study was to investigate the induced differentiative effects of G Rh2 on SMMC 7721 hepatocarcinoma cells. METHODS: Effects of G Rh2 on cell viability was analyzed by MTT assay. Cell morphology was examined by a light and electronic microscope. Alpha fetoprotein (AFP) in plasma was determined qualitatively and quantitatively with immunohistochemistry and ELISA. The specific activities of alkaline phosphatase (ALP) and heat resistant ALP in plasma were assayed by ALP kit based on Bessey method. The specific activity of γ glutamyltranspeptidase (γ GT) was measured with γ GT kit.The secretory amount of AFP or albumin was detected with radioimmunoassay kit. RESULTS: G Rh2 inhibited the proliferation of SMMC 7721 cells in dose and time dependent manners. The inhibition rate was 50.87%after 6 day treatment with 10 μg/ml G Rh2 while 46.84%after 4 day treatment with 20 μg/ml G Rh2. Twenty μg/ml G Rh2 induced the mature and normality of morphology and ultrastructure in SMMC 7721 cells. After treated with 10 μg/ml or 20 μg/ml G Rh2, the production of AFP was significantly reduced (P 0.05), and the secretory amount of AFP was reduced from 6.60±0.30 to 2.35±0.06 (P 0.01), and the specific activities of γ GT and heat resistant ALP were remarkably declined (P 0.01); while the secretory amount of albumin and ALP activity were remarkably enhanced (P 0.01). CONCLUSION: G Rh2 could induce the SMMC 7721 cell differentiation tending to normal.
【CateGory Index】: R735.7
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