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《Chinese Journal of Cancer》 2004-10
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Preparation and Feasibility of Superparamagnetic Dextran Iron Oxide Nanoparticles as Gene Carrier

CAO Zheng-Guo,ZHOU Si-Wei,SUN Kai,LU Xiong-Bing,LUO Gang,LIU Ji-Hong Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,430030,P.R.China  
BACKGROUND OBJECTIVE: Application of magnetic nano-particles as gene carrier in gene therapy of tumor has developed quickly. To obtain a new typ e non-viral gene introduction and therapy system,which is convenient,and can dr ive target gene to express highly and stably,this study was designed to explore the preparation of superparamagnetic dextran iron oxide nanoparticles(SDION),and the feasibility of SDION used as gene carrier in vitro. METHODS: SDION were pre pared by chemical co-precipitation,separated by gel filtration chromatography o n Sephacryl S-300HR,and centrifugation techniques,characterized by transmission electron microscopy,laser scattering system,and vibrating sample magnetometer s ignal processor. The green fluorescent protein-C2 (GFP-C2) plasmid was used as target gene. SDION-GFP-C2 compounds were synthesized by oxidation-reduction reaction. The connection rate of SDION and GFP-C2 was analyzed by agarose elect rophoresis,and evaluated by measuring concentration of GFP in the supernatant af ter centrifugation. Liposome transfection was used as control,the efficiencies o f SDION and liposome in transferring GFP gene into human bladder cancer BIU-87 cells were evaluated under fluorescence microscope in vitro. RESULTS: The diamet er of SDION ranged from 3 nm to 8 nm,the effective diameter was 59.2 nm,and the saturation magnetization was 0.23 emu/g. After oxidized by sodium periodate of 1 0 mmol/L,and deoxidized by sodium hydride boron of 0.5 mol/L,SDION could connect with GFP in maximum degree,the transfection efficiency of SDION as gene carrier was about 45%,even higher than that of liposome (about 30%). CONCLUSION: SDIO N could connect with GFP plasmid by oxidation-reduction reaction,and success to transfer GFP gene into human bladder cancer BIU-87 cells in vitro.
【Fund】: 国家自然科学基金项目(No.30271300)~~
【CateGory Index】: R-39
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