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《Chinese Journal of Cancer》 2004-12
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Induction of Apoptosis in Human Nasopharyngeal Carcinoma Cell Line CNE-2Z by Curcumin

LIANG Tong, CHEN Mei-Jun, ZHOU Ke-Yuan, TANG Xu-Dong, WANG Xu-Guang Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang,Guangdong,524023, P.R.China  
BACKGROUND OBJECTIVE: Nasopharyngeal carcinoma (NPC) is a commo n malignant tumor in southern China. Radiotherapy is a major therapy for NPC in ea rly phase,while proper chemotherapy is necessary for NPC in late phase. Some che motherapeutic drugs have been used to treat tumors by inducing apoptosis of tumo r cells. This study was to investigate effects of curcumin on proliferation,and apoptosis of NPC cell line CNE-2Z. METHODS: CNE-2Z cells were treated with dif ferent concentrations of curcumin,inhibition rates of cell proliferation,and IC5 0 of curcumin were detected by MTT method. Cell apoptosis was analyzed by flow c ytometry (FCM),agarose gel electrophoresis,and Hoechst33258-PI fluorescence sta ining. RESULTS: Proliferation of CNE-2Z cells was inhibited obviously by curcum in in dose-and time-dependent manners. IC50 of curcumin to CNE-2Z cells at 24 ,48,and 72 h were (24.05±0.47),(19.20±0.17),and (7.35±0.50)μmol/L,resp ectively. After treated with 5,10,and 20 μmol/L of curcumin for 24 h,apoptotic rates of CNE-2Z cells were (4.9±3.2)%,(10.7±2.7)%,and (14.7±0.5)%,r espectively. After treated with 10,20 μmol/L of curcumin for 24 h,morphologic c hanges,such as chromatin shrinkage,nuclear condensation,and chromatin fragmentat ion,were observed in CNE-2Z cells by fluorescent staining,a fragmented DNA ladd er was detected by electrophoresis. CONCLUSION: Curcumin may induce apoptosis,an d inhibit proliferation of CNE-2Z cells.
【Fund】: 广东省重点学科资助课题(No.GX9907)~~
【CateGory Index】: R739.63
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