Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cancer》 2005-10
Add to Favorite Get Latest Update

CpG Island Methylation of E-cadherin Gene Promoter in Gastric Carcinoma

ZHOU Yong-Ning1, XU Cai-Pu2, HAN Biao1, JI Rui1 1. Center of Endoscopy, The First Hospital, Lanzhou University, Lanzhou, Gansu, 730000, P. R. China 2. Department of Gastroenterology, Southwest Hospital, The Third Military Medical University, Chongqing, 400038, P. R. China  
BACKGROUND OBJECTIVE: Transcriptional silencing by CpG island methylation is now believed to be an important mechanism of carcinogenesis. E-cadherin can suppress tumor cell invasion and metastasis, and is considered as an invasion/metastasis suppressor gene. Inactivation of E-cadherin gene often occurs in poorly differentiated gastric carcinoma. This study was to investigate the CpG island methylation status of E-cadherin gene promoter in primary human gastric carcinomas and adjacent gastric tissues, and to explore the mechanism of gastric carcinogenesis. METHODS: The CpG island methylation status of E-cadherin gene promoter in 51 specimens of primary human gastric carcinoma, 37 specimens of adjacent gastric tissue, and 12 specimens of normal gastric mucosa was detected by methylation-specific polymerase chain reaction (MSP). The expression of E-cadherin was evaluated by immunohistochemistry. RESULTS: The CpG island methylaion was not detected in normal gastric mucosa, but detected in 32 (62.7%) gastric carcinoma specimens and 4 (10.8%) adjacent gastric specimens. It occurred more frequently in poorly differentiated carcinomas than in well differentiated carcinomas [72.2% (26/36) vs. 33.3% (6/15), P0.01], and it was presented at similar rates in stage T1/T2 carcinomas and stage T3/T4 carcinomas [55.6% (10/18) and 66.7% (22/33), P0.05]. Down-regulation of E-cadherin expression was detected in 84.5% (27/32) tumor tissues with methylated CpG island and 26.3% (5/19) tumor tissues with unmethylated CpG island. No statistical correlation was found between CpG island methylaion and Helicobacter pylori infection. CONCLUSIONS: The CpG island methylaion of E-cadherin gene promoter commonly exists in gastric carcinoma, particularly in poorly differentiated adenocarcinoma. E-cadherin promoter methylation may be involved in the early process of gastric carcinogenesis, but has no correlation to Helicobacter pylori infection.
【Fund】: 甘肃省科学事业经费(No.QS022-C33-029)~~
【CateGory Index】: R735.2;
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved