Inducement Effect of 9-Cis Retinoic Acid on Cell Cycle Arrest and Apoptosis of Gastric Carcinoma Cell Line MGC803 and Its Mechanism

XU Rui-Cheng, ZHOU Yan-Hong, HU Wen-Liang Department of Cellular Biology, Medical College of Chinese People's Armed Police Forces, Tianjin, 300162, P. R. China  

BACKGROUND OBJECTIVE: Antitumor effect of 9-cis retinoic acid (9-cis RA) on gastric carcinoma is unclear yet. This study was to explore the inducement effect of 9-cis RA on cell cycle arrest and apoptosis of gastric carcinoma cell line MGC803 and its mechanism. METHODS: The expression of RXRα, Cyclin D1, and CDK4 in MGC803 cells was detected by reverse transcription-polymerase chain reaction (RT-PCR). Cell cycle was detected by flow cytometry. The growth inhibition was analyzed by MTT assay. The apoptosis was detected by agarose gel electrophoresis and Hoechst33342/PI staining. The expression of apoptosis-associated gene Bcl-2 was detected by SP immunocytochemistry. RESULTS: When treated with 0.1-10 μmol/L 9-cis RA for 96 h, the proliferation of MGC803 cells was significantly inhibited. The proportion of MGC803 cells at G1 phase was significantly increased when treated with 10 μmol/L 9-cis RA for 48, 72, and 96 h, and showed an apparent G1 phase arrest. When treated with 9-cis RA for 72 h, typical apoptotic changes, such as chromatin condensation and DNA ladder, were observed in MGC803 cells. The expression of Bcl-2 was significantly decreased in MGC803 cells when treated with 10 μmol/L 9-cis RA for 48 h. RXRα expression was at a low level in MGC803 cells and up-regulated when treated with 10 μmol/L 9-cis RA for 48 h (P0.01). The expression of Cyclin D1 and CDK4 in MGC803 cells was both significantly down-regulated when treated with 10 μmol/L 9-cis RA for 96 h (P0.01). CONCLUSION: 9-Cis RA could induce G1 phase arrest and apoptosis in MGC803 cells through down-regulating the expression of cell cycle factors Cyclin D1 and CDK4.

【Key Words】： Gastric neoplasm MGC803 cells 9-Cis retinoic acid Cell cycle RXRα Apoptosis
【Fund】： 天津市自然科学基金项目(No.013804311)~~
【CateGory Index】： R735.2

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