Effects of Vimentin on Invasion and Metastasis of Prostate Cancer Cell Lines PC-3M-1E8 and PC-3M-2B4

WEI Jun-Cheng, WU Ming-Fu, ZHANG Yong-Tao, ZHAO Liang-Ping, LU Yun-Ping, MA DingDepartment of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, P. R. China  

BACKGROUND OBJECTIVE: Vimentin, a cytoskeleton protein, is involved in regulating the migration and proliferation of cells. This study was to detect the expression of vimentin in prostate cancer cell lines PC-3M-1E8 and PC-3M-2B4, and evaluate the effects of vimentin on invasion and metastasis of prostate cancer cells. METHODS: Two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption/time of flight mass spectrometry (MALDI TOF-MS) were used to detect the expression of vimentin in prostate cancer cell lines PC-3M-1E8 and PC-3M-2B4. Genetic intervention of vimentin gene and in vitro invasion assay were used to investigate the effects of vimentin on the invasion and metastasis of prostate cancer cells. RESULTS: The protein level of vimentin was higher in PC-3M-1E8 cells with high metastatic potential than in PC-3M-2B4 cells with low metastatic potential. Eukaryotic vectors expressing antisense- and sense-vimentin were constructed successfully and transfected into PC-3M-1E8 and PC-3M-2B4 cells separately. The number of invasive cells was significantly lower in PC-3M-1E8/vas cells with antisense-vimentin than in control PC-3M-1E8/3.1(-) cells (99.3±4.8 vs. 319.4±6.5, P0.01), and significantly higher in PC-3M-2B4/vs cells with sense-vimentin than in control PC-3M-2B4/3.1(+) cells (330.5±5.8 vs. 98.6±7.5, P0.01). CONCLUSION: Vimentin is a promising marker for predicting the invasion and metastasis of prostate cancer cells.

【Key Words】： Prostate neoplasm Invasion Metastasis Vimentin
【Fund】： 国家重点基础研究发展规划资助项目(No.2002CB513107)~~
【CateGory Index】： R737.25

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