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《Chinese Journal of Cancer》 2008-02
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Effect of DNA-PKcs Antisense Oligodeoxynucleotides on Radiosensitivity of Nasopharyngeal Carcinoma Cell Lines with Different p53 Function Statuses

JIANG Chang-Bin1,2,3, LIU Xiu-Fang1,2, HE Yu-Xiang1,2, NIU Dao-Li3, XIA Yun-Fei1,2 1. State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510060, P. R. China 2. Department of Radiation Oncology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P. R. China 3. Department of Radiation Oncology, The First Affiliated Hospital, Guangzhou Medial College, Guangzhou, Guangdong, 510230, P. R. China  
BACKGROUND OBJECTIVE: DNA-dependent protein kinase (DNA-PK) can repair DNA double-strand break. This study was to observe the effect of DNA-PKcs antisense oligodeoxynucleotides (ASODN) on the radiosensitivity of nasopharyngeal carcinoma (NPC) cell lines with normal or abnormal p53 functions. METHODS: DNA-PKcs ASODN was transfected into CNE-1 and CNE-1-wtp53 cells. These cells were irradiated with 0, 0.5, 1, 2, 4, 6, or 8 Gy X-ray. Cell survival was determined by clonogenic assay. The parameters D0, Dq, and N for the single-hit multitarget model and the parameters α, β, α/β, and SF2 for the linear-quadratic model were calculated to evaluate the changes of radiosensitivity. RESULTS: The α values before DNA-PKcs ASODN transfection were 0.03 in CNE-1 cells and 0.05 in CNE-1-wtp53 cells; the α values after transfection were 0.04 in CNE-1 cells and 0.27 in CNE-1-wtp53 cells. The SF2 before transfection were 0.73 in CNE-1 cells and 0.50 in CNE-1-wtp53 cells; the SF2 after transfection were 0.45 in CNE-1 cells and 0.21 in CNE-1-wtp53 cells. The D0 before transfection were 2.08 Gy in CNE-1 cells and 1.13 Gy in CNE-1-wtp53 cells; the D0 after transfection were 1.07 Gy in CNE-1 cells and 0.83 Gy in CNE-1-wtp53 cells. The Dq before transfection were 2.04 Gy in CNE-1 cells and 1.36 Gy in CNE-1-wtp53 cells; the Dq after transfection were 1.24 Gy in CNE-1 cells and 0.73 Gy in CNE-1-wtp53 cells. The parameter α of CNE-1 cells was increased after DNA-PKcs ASODN transfection, but the parameters SF2, D0, and Dq were decreased after transfection. CONCLUSION: DNA-PKcs ASODN could enhance the radiosensitivity of CNE-1 cells regardless of p53 function status.
【Fund】: 国家自然科学基金(No.30070237)~~
【CateGory Index】: R739.63
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