Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cancer》 2008-08
Add to Favorite Get Latest Update

Effects of Mitoxantrone on the Activity of Human Protein Kinase CK2 Holoenzyme

LI Chun-Mei1,2,LIU Xin-Guang2,LIN Xiao-Cong2,CHEN Xiao-Wen2,3,LIANG Nian-Ci2 1.Department of Biochemistry & Molecular Biology,Guangdong Pharmaceutical College,Guangzhou,Guangdong,510006,P.R.China 2.Institute of Biochemistry & Molecular Biology,Guangdong Medical College,Zhanjiang,Guangdong,524023,P.R.China 3.Institute of Pediatrics,Shenzhen Children's Hospital,Shenzhen,Guangdong,518026,P.R.China  
BACKGROUND OBJECTIVE:Protein kinase CK2,a highly conserved protein serine/threonine kinase that is ubiquitously distributed in eukaryotes,has a close relationship with human leukemia.Mitoxantrone is an effective drug used for acute leukemias.This study was to observe the effects of mitoxantrone on the activity of recombinant holoenzyme of human protein kinase CK2 and proliferation in human leukemia cell line HL-60.METHODS:The CK2 holoenzyme composed of α′ and β subunits was recombinanted in vitro.Subsequently,CK2 was treated with mitoxantrone at various concentrations,followed by addition of reacting liquid containing γ-32P ATP.CK2 activity was measured by detecting the radioactivity of 32P transferred onto the substrates of CK2.The effect of mitoxantrone on the proliferation of HL-60 cells was detected by the trypan blue dye exclusion assay;the changes in the cell cycle were analyzed by flow cytometry(FCM);apoptosis was analyzed by FCM and DNA agarose gel electrophoresis;effects of the drugs on the intrinsic CK2 activity were measured by a specific CK2 peptide substrate.RESULTS:Mitoxantrone strongly inhibited the holoenzyme activity of recombinant human protein kinase CK2(IC50=0.66 μmol/L).The inhibition of CKZ by mitoxantrone was competitive with respect to ATP(Ki 0.25 μmol/L) and mostly non-competitive with respect to casein(Ki 0.66 μmol/L).Mitoxantrone exerted strong cytotoxicity to HL-60 cells.When treated with 0.1 μmol/L mitoxantrone for 12 h,the apoptotic rate of HL-60 cells was 9.3%.Mitoxantrone did not affect intracellular CK2 activity.CONCLUSIONS:Mitoxantrone is a strong inhibitor of recombinant human protein kinase CK2 in vitro.Apoptosis induced by mitoxantrone in HL-60 cells has no correlation to intracellular CK2 activity.
【Fund】: 国家自然科学基金(No.30672205);; 广东省自然科学基金项目(No.011766 No.05011579);; 广东省科技计划项目(No.2002C30109)~~
【CateGory Index】: Q555
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 WAN Xue-rui;YANG Run-xia;WANG Chuan;LIU Gui-lin;WU Run;College of Veterinary Medicine,Gansu Agricultural University;;Effects of CK2 Inhibitor on Biological Behaviour of DF-1 Cells with High Expression and Suppression Expression of Chicken PrP~C[J];畜牧兽医学报;2016-05
2 LI Chun-mei1,LIU Xin-guang2,LIANG Nian-ci21Department of Biochemistry & Molecular Biology,Guangdong Pharmaceutical College,Guangzhou 510006,China;2Institute of Biochemistry & Molecular Biology,Guangdong Medical College,Zhanjiang 524023,China.;Effects of mitoxantrone on the proliferation of MCF-7 cell lines[J];现代肿瘤医学;2010-02
【Citations】
Chinese Journal Full-text Database 4 Hits
1 CHEN Xiao wen,LIU Xin guang\+*,LIANG Jing yao,LIANG Nian ci (Institute of Biochemistry and Molecular Biology,Guangdong Medical College,Zhanjiang\ 524023,China);Cloning,Expression of Human Protein Kinase CK2α′Subunit in E.coli and Purification and Characterization of Recombinant Protein[J];中国生物化学与分子生物学报;2004-02
2 HUANG Hua yi 1, ZHA Xi liang 2 (1.Department of Tumor Molecular Cell Biology, Guangxi Nationalities Hospital, NANNING GUANGXI 530001, China;2.Key Lab in Glycoconjugate of National Health Ministry and Department of Biochemistry, Shanghai Medical Coll;Development in research of antitumor effect of flavones compounds[J];中国新药与临床杂志;2002-07
3 LIU Xin Guang, LIANG Nian Ci( Institute of Biochemistry & Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China); MA Jian Quan(Department of Biochemistry , Sun Yat sen University of Medical Sciences, Guangzhou 510089, China).;Prokaryotic Expression, Purification and Characterization of Recombinant Human Protein Kinase CK2β Subunit[J];生物化学与生物物理进展;2000-02
4 LIU Xinguang 1) ,LIANG Nianci 1) ,MA Jianquan 2) ,LIU Wen 1) ( 1) Institute of Medical Biochemistry,Guangdong Medical College,Zhanjiang 524023; 2) Department of Biochemistry,Sun Yat sen University of Medical Sciences;Cloning and Sequencing of Recombinant cDNA Encoding Human Protein Kinase CK2 β Subunit[J];中国生物化学与分子生物学报;1999-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HOU Tao;SHI Li Ying;HE Jian;WANG Ping;WANG Ji Xia;WEI Lai;ZHANG Xiu Li;LIANG Xin Miao;School of Life Sciences and Technology, Dalian University;Dalian Institute of Chemical Physics, Chinese Academy of Sciences;;Discovery of Two flavonoids as Agonists of G Protein-Coupled Receptor-35[J];中国科学:生命科学;2016-02
2 ZHENG Xusheng;LI Huihui;LI Qi;TANG Yu;WANG Yigang;XU Chuanlian;College of Life Sciences,Zhejiang Sci-Tech University;School of Pharmacy,Tianjin University;;Anti-tumor Activity Anaysis of Several Natural Pyrones Derivatives[J];浙江理工大学学报;2016-01
3 YANG Qiu-ming;LI Jing-ya;XIAO An-feng;CHEN Jun;College of Food and Biological Engineering,Jimei University;Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province;Research Center of Food Biotechnology of Xiamen;Key Laboratory of Recycling Application and Deep Processing in Economic Marine Alga,Xiamen South Oceanographic Research Center;;Extraction and Purification of Naringin and Limonoids from Pomelo Peel[J];集美大学学报(自然科学版);2015-06
4 ZHU Xiao-qin;CHEN Pei;HUANG Zhong-di;QIU Zhong-chuan;ZHAO Lin;HU Xiao-ying;Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;;Research progress of traditional Chinese medicine in regulating blood tumor cell cycle[J];上海中医药杂志;2015-09
5 Saimire Ainiwaer;Mutalifu Aimaiti;Guzhalinuer Maitishadike;Reziwanguli Yuemaier;Abulizi Abudula;Department of Biochemistry,College of Basic Medicine;Key Laboratory of Molecular Biology and Endemic Diseases,Xinjiang Medical University;;The effect of galangal general flavonoids on the proliferation and cellular apoptosis of cervical carcinoma cells[J];新疆医科大学学报;2015-08
6 Chai Xiaojie;Liu Yiqiong;Wang Yiyun;Wu Tianxiang;Liu Liying;Dalian Ocean University/Key Laboratory of Mariculture & Stock Enhancement in North China's Sea,Ministry of Agriculture/Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province;;Prokaryotic Expression, Purification and Cloning of MAPKK Kinase Gene DsMAPKKK from Dunaliella salina[J];中国农学通报;2015-23
7 Zhuang Jingjing;Chen Wei;Li Ya;Shen Yang;Li Jianrong;College of Food Science and Biotechnology,Zhejiang Gongshang University;College of Biosystem Engineering and Food Science,Zhejiang University;College of Chemical Engineering and Food Safety,Bohai University;;Studies on Free Radical Scavenging and Oxidative Damage Protection Activities of Blackberry Flavonoid[J];中国食品学报;2015-07
8 LI Wei-xia;XI Dong-hua;Atawulla·tiemur;SU Yu-hong;WU Bin;College of Chemistry & Chemical Engineering, Xinjiang University;Institute of Agro-products Storage and Processing, Xinjiang Academy of Agricultural Sciences;;Content determination of total flavonoids and optimization of the extraction process of extracts from green husk of almonds[J];食品科技;2015-07
9 CHEN Ya-lan;WANG Xue-qing;LI Yue-jiao;SONG Wen-jun;WANG Su-ying;ZHAO Guo-qiang;FU Qing-wei;Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce;Qianxi Chestnut Industry Research and Development Center;;Effect of the flavonoids from chestnut flower on antioxidant activity and cellular viability of Hela line[J];食品工业科技;2015-14
10 BAI Shengwen;TANG Chao;TIAN Jing;YAN Hongfei;XU Xiaosha;FAN Huiling;College of Agriculture and Biotechnology, Hexi University;;Extraction and Antioxidant Activity of Total Flavonoids from Sea Buckthorn Pomace[J];食品科学;2015-10
【Co-references】
Chinese Journal Full-text Database 4 Hits
1 ZHU Man-ling;WU Run;YU Hong-wei;WAN Xue-rui;LIU Lei;LIU Xia;ZHANG Xiao-li;JIA Xiao-rui;College of Veterinary Medicine,Gansu Agricultural University;;Establishment of SYBR GreenⅠreal-time RT-PCR for detection of chicken Akt gene mRNA[J];中国兽医科学;2014-07
2 LI Chun-Mei1,2,LIU Xin-Guang2,LIN Xiao-Cong2,CHEN Xiao-Wen2,3,LIANG Nian-Ci2 1.Department of Biochemistry & Molecular Biology,Guangdong Pharmaceutical College,Guangzhou,Guangdong,510006,P.R.China 2.Institute of Biochemistry & Molecular Biology,Guangdong Medical College,Zhanjiang,Guangdong,524023,P.R.China 3.Institute of Pediatrics,Shenzhen Children's Hospital,Shenzhen,Guangdong,518026,P.R.China;Effects of Mitoxantrone on the Activity of Human Protein Kinase CK2 Holoenzyme[J];癌症;2008-08
3 LIN Xiao-cong1, LI Chun-mei2, LIU Xin-guang1,CHEN Xiao-wen3, LIANG Nian-ci1(1.Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang Guangdong 524023,China; 2.Dept of Biochemistry, Guangdong College of Pharmacy, Guangzhou 510240,China; 3. Pediatrics Institute of Shenzhen Children's Hospital, Shenzhen Guangdong 518026, China);Effect and structure-activity study of nine kinds of anthraquinone derivatives on recombinant human protein kinase CK2 holoenzyme[J];中国药理学通报;2007-06
4 ZHANG Zhi rong, GONG Yan, HUANG Yuan, HE Qin (School of Pharmacy, West China University of Medical Science, Chengdu 610041, China);CONJUGATION OF MITOXANTRONE LOADED NANOSPHERES AND ANTI-C-erbB-2 MONOCLONAL ANTIBODIES[J];药学学报;2001-02
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 LIU Xin Guang, LIANG Nian Ci( Institute of Biochemistry & Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China); MA Jian Quan(Department of Biochemistry , Sun Yat sen University of Medical Sciences, Guangzhou 510089, China).;Prokaryotic Expression, Purification and Characterization of Recombinant Human Protein Kinase CK2β Subunit[J];生物化学与生物物理进展;2000-02
2 LIU Xin guang,LIANG Nian ci (Institute of Medical Biochemistry,Guangdong Medical College,Zhanjiang 524023) MA Jian quan (Department of Biochemistry,Sun Yat sen University of Medical Sciences,Guangzhou 510089);Prokaryotic Expression,Purification and Characterization of Recombinant Human Protein Kinase CK2α Subunit[J];中国生物化学与分子生物学报;2000-01
3 LIU Xinguang 1) ,LIANG Nianci 1) ,MA Jianquan 2) ,LIU Wen 1) ( 1) Institute of Medical Biochemistry,Guangdong Medical College,Zhanjiang 524023; 2) Department of Biochemistry,Sun Yat sen University of Medical Sciences;Cloning and Sequencing of Recombinant cDNA Encoding Human Protein Kinase CK2 β Subunit[J];中国生物化学与分子生物学报;1999-02
Similar Journals
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved