Changes of plasma and tissue myelopetoxidase activity in hypovolemic shock combined with endotoxin challenge
LI Jun you SUN Shi rong YAN Ming (Burn institute,304 th hospital of PLA, Beijing 100037)
To investigate the changes of myeloperoxidase (MPO)activity in blood and tissue from rats induced by hemorrhage combined with endotoxin challenge. A rat model of endotoxemia wete reproduced by henorrhagic shock combined with endotoxin infusion. MPO activity in blood and tissue wete dctermined.The influence of venous plasma on the expression of CD11b and CD18 on PMN was measured by ELISA. Lungs MPO actirty were markedly increased at post reperfusion and got to peak level after hemorrhagic shock post reperfusion could stimulate up regulation of CD11b and CD18 on PMN. MPO activities were significantly increased in tissue during hypovolemic shock combined with endotoxin challenge.The results suggest that PMN aggregated and activated in tissues is one of the main causes leading to the damage of organs.
【Fund】： 全军“九五”医学科研规划指令性课题!(96L0 5 3)