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《Amino Acids & Biotic Resources》 2004-01
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Effects of Carbachol on Myelopetoxidase Activity of Rat Intestine During Gut Ischemia-Reperfusion

JIANG Xiao-Guo, LI Jun-you, HU Sen (Burn Institute, 304th Hospital of PLA, Beijing 100037, China)  
To investigate the effects of carbachol on myelopetoxidase activity and malondialdehyde and its relation to some parameters concerning gut injury in rats during gut ischemia-reperfusion, Wistar rats were divided into three groups, pretreated group (carbachol intramuscular injection at 30min after SMAO 0.1mg·kg -), treated group (carbachol intramuscular injection at 30min from begining reperfusion), and controls (saline injection ). Myelopetocidase , malondialdehyde, and TNF-α of gut tissue were determined at 1h, 2.5h and 6h after SMAO. The results showed that the MPO activity, contents of MDA and TNF-α significantly decreased in pretreated and treated groups than those in the control after the carbachol intramuscular injection ( P0.05 ). However, there is no difference between pretreated group and treated group. The results suggested that carbachol restrained the proinflammatory cytokine(TNF-α) release, and reduced the concentration of postshock systemic neutrophil (PMN ) in gut tissue , and depressed the myelopetoxidase activity.
【Fund】: 全军医学科学技术研究“十五”计划指令性课题基金资助项目 (编号 0 1L0 81)
【CateGory Index】: R965
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