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《Amino Acids & Biotic Resources》 2004-03
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Application of ISSR Technique to Genetic Research of Lotus Root

WANG Lan 1* , HAN Yan-chuang 1* , PENG Yu-lu 1 , TENG Cai-zhu 1 , ZHOU Ming-quan 2** , HU Zhong-li 1, 2** , SONG Yun-chun 1 ,2 (1 Key Lab of the Ministry of Education for Plant Developmental Biology, Wuhan University, Wuhan 430072;2 Lotus center , Wuhan University, Wuhan 430072, China)  
The ISSR analysis was conducted in 12 lotus. From 65 random primers, 7 primers were selected and then amplified the 12 genomic DNAs. 91 RAPD fragments were generated, 75 of them were polymorphic marks. Every primer offered 13 markers on average. The molecular phylogenetic tree conducted by UPMGA method and showed the genetic relationship among 12 lotus, which was better identical with pedigree analysis.
【Fund】: 国家科技攻关项目 ( 2 0 0 2BA5 46C);; 湖北省科技攻关重大项目 ( 2 0 0 2AA2 0 5A );; 武汉市科技攻关重点项目( 2 0 0 2 2 0 0 5 13 2 )
【CateGory Index】: S645.1
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