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《Amino Acids & Biotic Resources》 2006-01
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Cluster Analysis of Nelumbo Based on SRAP Markers

LIU Yue-guang~(1*),TENG-Yong yong~(3*),PAN Chen~1,HAN Yan-chuang~1,ZHOU Ming-quan~2,HU Zhong-li~(1,2**)(1 Key Lab of the Ministry of Education for Plant Developmental Biology,Wuhan University,Wuhan 430072;2 Lotus center,Wuhan University,Wuhan 430072;3 College of Life Sciellce,Xinyang Nomal University,Xinyang 46400,China)  
A newly developed molecular marker-SRAP(sequence-related amplified polymorphism) was used to detect DNA polymorphisms among the 17 lotus accessions.7 pair primers were used to amplify the 17 genomic DNA.168 polymorphic bands were generated in total,159 of them were polymorphic bands,with an average of 24 marker bands per pair of primers.The dendrogram generated by UPMGA showed the genetic relationship among 17 accessions.It indicated that:(1) there were obvious differences between Nelumbo nucifera and Nelumbo lutea;(2) there was obvious genetic differentiation between rhizome and flower lotus accessions in the Nelumbo,among the flower lotus hybrids of Nelumbo nucifera and Nelumbo lutea and the other flower lotus accessions showed obvious differences;(3) SRAP markers should be a good marker for molecular mapping,but the closer accessions were not easy to be distinguished by SRAP analysis.
【Fund】: 国家自然科学基金项目(30471114);; 国家科技攻关项目(2002BA546C);; 湖北省科技攻关重大项目(2002AA205A);; 武汉市科技攻关重点项目(20022005132)资助
【CateGory Index】: S645.1
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