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《Amino Acids & Biotic Resources》 2006-02
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The Comparison of Expressions of Endoinulinase Gene from Aspergillus niger and Its Synthesized Gene in Yarrowia lipolytica

ZHU Lin,WANG Bo,CHEN Jing,SHU Wang-yun,JIANG Si-jing,MA Li-xin(College of Life Science,Hubei University,Wuhan 430062,China)  
By using the principle of preference of the yeast codon,the endoinulinase gene was changed into its synthesis gene of preference of the yeast codon.These two genes were cloned into the vector of the expression ofYarrowia lipolytica pINA1296,and the recombinant vectors pHBM2020 、pHBM2021 were obtained.The two recombinant vectors were transferred into Yarrowia lipolytica CLIB725,and the two recombinant strains CLIB725(pHBM2020)、CLIB725(pHBM2021)were constructed.The two strains were cultured in the flask.And the analysis of the SDS-PAGE and the enzyme activity showed that the two genes were expressed in Yarrowia lipolytica,and the enzyme activity of synthesis gene of the endoinulinase was higher than that of the original endoinulinase gene.
【CateGory Index】: Q78
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Guo-Qing CUI Fu-Mian YANG Xiu-Qing QIAN Shi-Jun * (Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China);Purification and Properties of Endoinulinase from Chaetomium sp.[J];Acta Microbiologica Sinica;2004-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Hao Linhua, Wang Xiaobin, Chen Kaoshan, Li Guangyou;The Research Advances and Application on Functional Oligosaccharides[J];Feed Industry;2005-12
2 ZHU Lin,CHEN Jing-jing,SHU Wang-yun,WANG Bo,MA Li-xin(School of Life Science,Hubei University,Wuhan 430062,China);The expression of exoinulinase from Kluyveromyces marxianus in Pichia pastoris[J];Journal of Hubei University(Natural Science);2006-04
3 ZHAO Xiao-chuan~1,WANG Zhuo-long~2,SUN Jin-yan~1(1.Animal Husbandry Research Center of Heilongjiang Academy of Agricultural Sciences,Harbin 150086;2.Mudanjiang Animal Quarantine Station,Mudanjiang 157009);The Application of Jerusalem Artichoke in Livestock Production[J];Heilongjiang Agricultural Sciences;2006-06
4 WU Lan,YANG Jin ping (College of Life Science,Nanchang University,Jiangxi Nanchang 330047,PRC);The Study on Yarrowia Lipolytica for Oil-containing Wastewater Degradation[J];Jiangxi Energy;2003-04
5 WU Lan,GE Gang,LUO Yu_ping(College of Biology Science Nanchang University,Jiangxi Nanchang,330047 PRC);Recent Progress on Bio-degradation of Grease-wastewater[J];Jiangxi Science;2003-04
6 SUN Ji-lu,JIA Ying-min,TIAN Hong-tao,LI Ying-jun,SANG Ya-xin(Food Science and Technology College,Agricultural University of Hebei,Baoding 071001,China);Screening and Optimizing of Bifidobacterium Medium Jerusalem Artichoke Juice[J];Food Science;2003-11
7 YANG Lian1,JIANG Bo1,*,FENG Biao2,JING Zheng-yu2 (1.Key Laboratory of Food Science and Safety, Ministry of Education, Southern Yangtze University, Wuxi 214036, China;2,School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China);Electrodialysisi nC rudeI nulinP urification[J];Food Science;2006-07
8 DONG Ying, ZHANG Ju-fen, ZHANG Hong-yin (School of Food and Biological Engineering,Jiangsu University, Zhenjiang 212013, Jiangsu, China);THE RESEARCH DEVELOPMENT ON PRODUCTION OF INULINASE FROM MICROOGANISM[J];Food Research and Development;2006-10
9 SUN Ji-lu JIA Ying-min SANG Ya-xin(Food Science and Technology College,Agricultural University of Hebei,Baoding,071001);The Development and Utilization of Jerusalem Artichoke Resource[J];Food Science and Technology;2003-01
10 DENG Jian-zhen,WEI Hong-qun,CHEN Yan-zhen(Guangxi Normal University,Guilin 541004,China);Screening and culture condition optimization of inulinase-producing Aspergillus Niger strains[J];Journal of Biology;2007-06
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Wei WenLing Xie Zhong Wang QunLi Cheng Yingxia(Department of Biology, Xiamen University, Xiamen);Production and Properties of Penicillium Inulinase[J];Industrial Microbiology;1994-04
2 Xie Qiuhong(Department of Molecular Biology, Jilin University, Changchun, 130023)Xiang Hongyu(Bethune Medical University, Changchun, 130021 );Screening and Primary Identification of Microbes Hydrolysing Inulin into Fructose[J];ACTA SCIENTIARIUM NATURALIUM UNIVERSITATIS JILINENSIS;1996-03
3 Xie Qiuhong, Xiang Hongyu, Ge Yubin, Yin Hut(Department of Molecular Biology, Jinn University, Changchun, 130023);Screening of Inulinase-Producing Strain and Fermentation Conditions[J];ACTA SCIENTIARIUM NATURALIUM UNIVERSITATIS JILINENSIS;1996-04
4 ;Functional Feed Additive:Fructo oligosaccharides[J];CEREAL & FEED INDUSTRY;1997-07
5 LUO Ying 1 LI Jun gang 2 ( 1. Department of Biology, Mianyang Teacher’s College, Mianyang 621000, Sichuan; 2. Mianyang Research Center of Biotechnology, Mianyang 621000, Sichuan);Study on Enzymolysis of Jerusalem Artichoke and Its Comprehensive Utilization[J];JOURNAL OF SICHUAN NORMAL UNIVERSITY(NATURAL SCIENCE);1999-06
6 WANG Jian hua 1 LIU Yan yan 2 YAO Bin 1 WANG Ya ru 1 1(Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081) 2(Wuhan Institute of Medicine Design,National Administration Office of Medicine,Wuhan 430061);A Study on Screening and High Density Cell Cultivation of A Yeast Strain Kluyveromyces with High Inulinase Yielding and Its Enzymology Properties[J];Chinese Journal of Biotechnology;2000-01
7 Wang Huilian; Song Guoyong; Li Hong(Scientific Research Institute of Food and Fermentation Industry,Ministry of Light Industry,Beijing);Studies on Aspergillus niger No.26 Inulinase[J];FOOD AND FERMENTATION INDUSTRIES;1994-03
8 Jia Yingmin(Department of Food Science, Hebei Agricultural University, Baoding 071001)Zhao Xuehui(Department of Food Science and Technelogy, Huazhong Agricultural university, Wuhan 430070);PURIFICATION AND PROPERTIES OF INULINASES FROM ASPERGILLUS NIGER M89[J];ACTA MICROBIOLOGICA SINICA;1998-02
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