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《Amino Acids & Biotic Resources》 2006-02
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The Comparison of Expressions of Endoinulinase Gene from Aspergillus niger and Its Synthesized Gene in Yarrowia lipolytica

ZHU Lin,WANG Bo,CHEN Jing,SHU Wang-yun,JIANG Si-jing,MA Li-xin(College of Life Science,Hubei University,Wuhan 430062,China)  
By using the principle of preference of the yeast codon,the endoinulinase gene was changed into its synthesis gene of preference of the yeast codon.These two genes were cloned into the vector of the expression ofYarrowia lipolytica pINA1296,and the recombinant vectors pHBM2020 、pHBM2021 were obtained.The two recombinant vectors were transferred into Yarrowia lipolytica CLIB725,and the two recombinant strains CLIB725(pHBM2020)、CLIB725(pHBM2021)were constructed.The two strains were cultured in the flask.And the analysis of the SDS-PAGE and the enzyme activity showed that the two genes were expressed in Yarrowia lipolytica,and the enzyme activity of synthesis gene of the endoinulinase was higher than that of the original endoinulinase gene.
【CateGory Index】: Q78
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