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Screening of L-Serine Producer and Biosynthesis of L-Serine in Resting Cell Culture System

OU Qian,WEI Dong,WU Bo(1.The College of Life Science and Technology,Guangxi University.2.Key Laboratory of Microbial and Plant Genetic Engineering of Ministry of Education.3.Key Laboratory of Subtropical Bio-resources Conservation and Utilization of Guangxi,Nanning 530005,China)  
A L-serine producing bacterium strain,named as MB200,was isolated from digester remainder in Longan,Guangxi.This strain can grow in the medium with methanol as sole carbon source.Traditional taxonomy,assays of its 16S rDNA sequence homology identified MB200 as Methylobacterium sp.The optimum culture conditions for the strain were:temperature32℃,pH8.0,methanol concentration 1.25%.Resting cells system was set up for producing Lserine by MB200.After optimizing the resting cells culture conditions,the optimum conditions were obtained: glycine 10 g·L~(-1),methanol 50 g·L~(-1), cell density 30 g·L~(-1),pH8.9,shaker speed 250 rpm,temperature32℃,resting cells incubation time 48 h.Under above conditions,7.2 g·L~(-1) L-serine was produced by this bacterium.
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