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《Amino Acids & Biotic Resources》 2007-01
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Determination of Serine by Chromotropic Acid-Spectrophotometry

ZHOU Yuan-yuan,ZHU Min,WANG Lei,YU Long-jiang*(Institute of Resource Biology and Biotechnology,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China)  
A spectrophotometry method of determining content of serine has been set up.Serine has a reaction with chromotropic acid after oxidized by sodium periodate under certain pH condition.The production showed an absorption peak at 570 nm,and was linear with the content of Serine.The results showed that the linear range was between 0 and 1.0 g·L-1(r=0.9973) and the average recovery was 100.30% with corresponding RSD of 1.11%(n=5).Compared with HPLC,the accordant rates of this method and HPLC reached between 99% and 101%.The spectrophotometer method is simple,convenient,accurate and less disturbed.
【CateGory Index】: Q517
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