Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Amino Acids & Biotic Resources》 2007-02
Add to Favorite Get Latest Update

Determination of AFP in Trace by Resonance Rayleigh Scattering Spectra of Phosphato-Molybdate Heteroply Acid

GAN Ning*,HOU Lin-xi,LI Tian-hua,XU Wei-Min,ZHOU Xiong(The State Key Laboratory Base of Novel Functional Materials and Preparation Science,Ninbo University,Ningbo 315211,China)  
When protein reacted with some large inorganic acid anions such as molybdate to form new products,a maximum resonance scattering(RS) peak at 480 nm for the system of protein-phosphomolybdic acid(PMA) was observed,with 3 other less stong peaks at 400,570,740nm.So a new resonance scattering spectroscopic method has been developed for the quantitative determination of AFP.The optimum conditions,effective factors,the relation between the scattering intensities and the concentration of protein,and the sensitivity of the method have been investigated.The detective limit of different protein-phosphomolybdic acid varied from 5.2 to 78ng·mL-1.The sensitivity of molybdate-protein system was the highest,and the detective limit of AFP was 2.5ng·mL-1.The method has advantages of easy operation,high sensitivity and wide determination range.Proteins in synthetic samples have been determined by the method with satisfactory results.
【Fund】: 浙江省科技攻关项目(No.2006C31040);; 宁波市自然科学基金(No2006A610048)资助项目
【CateGory Index】: R446.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 LIU Shao-pu;LIU Zhong-Fang(Institute of Environmental Chemistry,Southwest China Normal University,Chongqing,630715);Spectra of Resonant Luminescence and Double Scattering of Mercury(Ⅱ)-Thiocyanate-Victoria Blue 4R System and Their Analytical Application[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1996-06
2 Zhou Dongmei, Zou Hanfa *, Yang Li, Jia Lingyun and Zhang Yukui (National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian,116011);Determination of HIgG in Human Plasma by High Performance Membrane Affinity Chromatography(HPMAC)[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1998-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIANG Rong-yuan(Xiamen Municipal Environmental Monitoring Station,Xiamen 361004);Determination of Mercury Using Resonance Light-scattering Method[J];Environmental Science & Technology;2006-03
2 Liu Shaopu; Yang Rui; Liu Zhongfang (Institute of Environmental Chemistry, South- Normal University, Chongqing Chongqing 400715);Resonance Rayleigh Scattering and Second-Order Scattering of Chromium(Ⅵ )-Iodide-Victoria 4R System and Its Analytical Application[J];Chinese Journal of Analytieal Chemistry;1998-12
3 Li Yuanfang; Huang Chengzhi; Hu Xiaoli (Institute of Environmental Chemistry, Southwest Nounal University, Chongqing 400715);The Principles and the Applications of Resonance Light Scattering Technique in the Research of Biochemistry and Bioanalysis[J];Chinese Journal of Analytieal Chemistry;1998-12
4 Zhou Dongroei, Zou Hanfa , Yang Li, Ni Jianyi, Wang Hailin, Zhang Yukui( National Chromatographic Research & Analysis Centre, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011);Highly Efficient Separation of Proteins Using High Performance Ion Exchange Membrane Chromatography[J];Chinese Journal of Analytical Chemistry;1999-12
5 Yang Rui,Liu Shaopu (Institute of Environmental Chemistry, Southwest China Normal University,Chongqing 400715);Development of Some Molecular Spectral Analytical Methods for the Determination of Proteins[J];Chinese Journal of Analytieal Chemistry;2001-02
6 Luo Quanzhou, Zou Hanfa *, Wang Hailin, Mao Xiqin, Kong Liang, Ni Jianyi (National Chromatographic Research & Analysis Centre, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011);Preparation and Characterization of Protein A Immobilized on Molded Monolithic Rod of Macroporous Poly(glycidyl methacrylate-co-ethylene dimethacrylate) for Affinity Chromatography[J];Chinese Journal of Analytieal Chemistry;2001-05
7 Shen Jinshan * (Department of Chemistry, Hebei Normal University, Shijiazhuang 050016) Li Xianrui (College of Fundamental Medicine, Hebei Medical University, Shijiazhuang 050017);Determination of Cr Ⅵand CrⅢin Environmental Water by Resonance Scattering with the Ion Association Complex of Rhodamine B and I_3~-[J];Chinese Journal of Analytieal Chemistry;2001-08
8 Hu Qinghong 1, Jiang Bo 2 (Department of Basic Chemistry 1, Department of Pharmaceutical Chemistry 2, Zunyi Medical College, Zunyi 563003);Resonance Rayleigh Scattering of Anionic Surfactant-protein Systems and Their Analytical Application[J];Chinese Journal of Analytical Chemistry;2003-09
9 GAO Jian-hua~(*), LI Hua-cen, YANG Lu, WANG Jun-cheng, GONG Xue-yun (Department of Chemistry, Zhengzhou University, Zhengzhou 450052);Double Scattering and Antidouble Scattering Spectra for the Determination of Human Serum Albumin with Tetrasulponaeted Ferric Phthalocyanine[J];Journal of Analytical Science;2005-01
10 JIANG Bo * and HU Qing hong (Department of medicinal Chemistry , Zunyi Medical College, Zunyi 563003);Determination of proteins by resonance rayleigh scattering with dodecyl benzene sodium sulfonate[J];Analytical Laboratory;2002-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YIN Ge ping 1, LI Qiang 2, CHEN Song fen 1, et al 1 The Jinan Military General Hospital, Jinan 250031,P R China 2 The Second Affiliated Hospital of Shandong Medical University, Jinan 250013,P R China $$$$;The FCM study of tumour cellular apoptosis in patients with ovarian carcinoma before and after chemotherapy[J];CHINESE JOURNAL OF CANCER;2000-04
2 XIE Da-Xing, WU Jian-Hong, FENG Yong-Dong, LI Xiao-Lan, TAO De-Ding, GONG Jian-PingCancer Research Institute,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, P.R.China;Analysis of Cell Cycle Checkpoints by Cyclins/DNA Flow Cytometry[J];Chinese Journal of Cancer;2005-09
3 Li Chenxu, Liu Hongyang, Liu Cheng,Sun Changjie(1 The Central laboratoy,The School of Preventive Medicine;2 The First Clinical College. ; 3 Changchun Hospital of Tuberculosis);Diagnosis of HBsAg by immunochromatography[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1998-04
4 CAI Jia-li~1;WU Sheng-xi~1;HU Ren-jian~1;JIANG He~1;WANG Jian~2(1.School of Bioengineering,Chongqing Institute of Technology,Chongqing 400050,China;2.Agricultural Burean of Chongqing,Chongqing 400015,China);Development of a Colloidal Gold-immunochromatography Assay for Rapid Diagnosis of Avian Influenza[J];Journal of Chongqing Institute of Technology;2006-11
5 CAI Qiang WU Weiming CHEN Yuquan (Biosensor National Special Lab, Zhejiang University,Hangzhou 310027, P.R.China);The Method and Immuno-application of Electronic Detection Based on Nanogold[J];Journal of Transcluction Technology;2003-02
6 GUO Hua-Zhang; YIN Ying; LIU Jian; HUYun-Yu; ZHANG Ying-Hua(Department of Orthopedics, Xijing Hospital,Fourth Military Medical University, Xi'an 710038)(Central Laboratory, Tangdu Hospital, FourthMilitary Medical University);Analysis of the T lymphocyte subsets in the blood of RBX transplantation patients with flow cytomctry[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1998-05
7 YANG Chun-li, QIU Yu-rong Clinical Laboratory, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China;Detection of cellular immune function of Systemic Lupus Erythematosus patients by flow cytometry[J];Journal of First Military Medical University;2000-03
8 ZHENG Guoqiang, LIU Xuyi, HAN Fusheng, XU Guangwei. Department of Surgery, The School of Oncology, Peking University, Beijing 100036, P.R.China;The analysis of multi-drug resistance of doxorubicin with flow cytometry[J];Chinese Journal of Lung Cancer;2002-01
9 XIE Ji yun, JIANG Zhi liang, ZHONG Fu xin, FENG Zhong wei, GUO Xiu jiao (Colege of Chemistry and Life Science, Guangxi Normal University, Guilin 541004, China);Spectral Properties of Blood Cell[J];ANALYSIS AND TESTING TECHNOLOGY AND INSTRUMENTS;2000-03
10 JIANG Zhi liang (Institue of New Technology and Material, Guangxi Normal University, Guilin 541004, China);Study of the Nonlinear Scattering Equation for Au Nanoparticle in Solution[J];Analysis and Testing Technology and Instruments;2000-04
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Liu, Shao-Pu Liu, Zhong-Fang Li, Kang-Ye(Institute of Environmental Chemistry, Southwest China Normal University, Chongqing, 630715);Fluorescence Reaction of Cationic Surface-Active Agents with Eosin Y and Their Analytical Applications[J];Acta Chimica Sinica;1995-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved