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《Amino Acids & Biotic Resources》 2007-02
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Determination of AFP in Trace by Resonance Rayleigh Scattering Spectra of Phosphato-Molybdate Heteroply Acid

GAN Ning*,HOU Lin-xi,LI Tian-hua,XU Wei-Min,ZHOU Xiong(The State Key Laboratory Base of Novel Functional Materials and Preparation Science,Ninbo University,Ningbo 315211,China)  
When protein reacted with some large inorganic acid anions such as molybdate to form new products,a maximum resonance scattering(RS) peak at 480 nm for the system of protein-phosphomolybdic acid(PMA) was observed,with 3 other less stong peaks at 400,570,740nm.So a new resonance scattering spectroscopic method has been developed for the quantitative determination of AFP.The optimum conditions,effective factors,the relation between the scattering intensities and the concentration of protein,and the sensitivity of the method have been investigated.The detective limit of different protein-phosphomolybdic acid varied from 5.2 to 78ng·mL-1.The sensitivity of molybdate-protein system was the highest,and the detective limit of AFP was 2.5ng·mL-1.The method has advantages of easy operation,high sensitivity and wide determination range.Proteins in synthetic samples have been determined by the method with satisfactory results.
【Fund】: 浙江省科技攻关项目(No.2006C31040);; 宁波市自然科学基金(No2006A610048)资助项目
【CateGory Index】: R446.1
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