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《Amino Acids & Biotic Resources》 2009-03
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Development of Indirect ELISA for Detecting Cow Mastitis Vaccine

ZHANG Jie(College of Biological & Environmental Science,Zhejiang Wanli University,Ningbo 315100,China)  
An indirect ELISA was developed by optimizing the concentraction of the coating antigen,and the incubating time of the serum and the sheep-against-cow IgG labeled by HRP.The developed indirect ELISA was primarily applied for detecting the antibody against mastitis.The results showed that the optimal coating concentration was 5 μg·mL-1 for Staphylococcus aureus,10μg/·mL-1 for Streptococcus agalactiae and 10μg·mL-1 for Streptococcus dysgalactiae.The optimal coating time was 12h at 4℃.The optimal reaction time of the 2% human albumin and the sheep against cow IgG was 30min.The results also indicated that the cow mastitis vaccine had protection effect when the antibody titer was double after cow being immune.
【CateGory Index】: S858.23
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