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《Amino Acids & Biotic Resources》 2010-01
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Optimization of Extraction Technology of Total Flavonoids from Rhodiola sacra by Response Surface Analysis

HUANG Ren-fu,WU Zhou-he,HAN Chun-cheng,ZHU Yi-ting(College of Bioengineering,Hubei University of Technology,Wuhan 430068,China)  
The factors influencing extraction of flavones from Rhodiola sacra were studied by single-factor experiments.Then response surface methodology(RSM)of Box-Behnken was used to optimize the above critical factors.The optimum levels of the factors were calculated through regressive method and the result as follows: samples were hydrolyzed by using the ratio of liquid to material 40:1(ml/g),cellulase of 1.2%,ultrasonic time of 12min for 3 times,ultrasonic powerful strength of 250W,at45℃and pH 5.0 for 4.5hours.Under the optimum levels,the extraction rate of flavones was up to 3.99%.
【CateGory Index】: TQ461
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