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Measurement of the Content of Tryptophan in Feather With Fluorescence Method

Jin Jingfang;Shen Mingquan(Shanghai Agricultural College 201101)  
This paper probes into the fluorescene analysis method of tryptophan in feathers.2~5mg feather sample was placed in the polytetrafluoroethylene tube.1 ml of 5mol/L NaOH comtaining 0.5% soluble starch was used as hydrolysate with pure nitrogen as protective gas.Hydrolyzate was hydrolyzed at 110℃ for 20 hours and neutralized with 6mol/L HCl.Then it was diluted five times with the buffer Na2HPO4-NaOH (pH11).The intensity and concentration of the fluorescence were 280nm of stimulated wavelength and 362nm of fluorescene wavelength.The results were reproducible.Compared with the standard,its deviation was 0.67%~3.01%.The recovery rate of samples was 95.3%~102.1%.
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