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Optimization of Amino Acids Analysis Using Precolumn Phenyl Isothiocyanate Derivatization and Reversed phase HPLC

Li Rui ping Teachers' College, Hubei Three Gorges University, Yichang 443000 Jiang Shou gui Zhao Jin Environmemtal Institute, Hebei University, BaoDing 071002  
The optimal parameters which influence the determination of amino acids using the PICO TAG method was described. The influence of the PH in the mobile phase, MeCN concentration, etc. were fully studied. The column contamination and its regeneration were also described. Linearity of response of amin acid content was studied in the range of 1.25 to 1500pmol, with correlation coefficients exceeding 0.999. The precision of the retention times of the phenylthiocarbamoyl(PTC) amino acids expressed as the coefficients of deviation was less than 0.5% for most of the amino acids in this linear range. The quantitaition of the amino acids was based on peak area, with coefficients of deviation tanging from 0.5 to 1.5%.
【CateGory Index】: Q517,
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