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Cloning of Hal1 gene and construction of its expression plasmid

SHEN Yan1,2 CHEN Xia1 CHEN Yan GONG Yi1 CHEN Yu-guang2(1Research Center of Biotechnology Shanghai Institutes for Biological Sciences the chinese Academy of Sciences,Shanghai 200233;2School of life Sciences,Shanghai University)  
Taking Saccharomyces cerevisiae BWG1-7A genomic DNA as template,Hal1 gene is amplified by PCR.The amplified product was digested with BamHI and EcoRI enzymes,then ligated into pYES2 vector.The recombinant pYES-Hal1 was successfully constructed and verified by DNA sequence,which provided a foundation of the construction of plant expression vector.
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