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Optimization of SRAP in Carrot

DENG Bo-tao ZHAO Fei WANG Xue-ying (College of Biological Sciences and Technology,Shenyang Agricultural University,Shenyang Liaoning 110161)  
Through optimization of concentration of Mg2+,dNTPs,primers and template DNA,SRAP-PCR of carrot was established.The results showed that in the 20μL reaction mixture the optimum concentration of genomic DNA template,Mg2+,primers and dNTPs were 3.00ng/mL,2.25mmoL/L,0.30μmoL/L and 0.20mmoL/L.
【CateGory Index】: S631.2
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 Chen Wansheng Wang Yuanying Luo Chenggang Yang Aiguo Jang Caihong Fan Jingyuan Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101;Optimization for SRAP-PCR System of Tobacoo Based on Orthogonal Design[J];Molecular Plant Breeding;2008-01
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Optimization of SRAP System in Maize[J];Journal of Anhui Agricultural Sciences;2006-15
2 ZHAO Xin-liang et al (Northwest A&F University,Yangling,Shaanxi 712100);Study on Classification of Maize Inbred Lines in Henan with SRAP Marker Technology[J];Journal of Anhui Agricultural Sciences;2007-07
3 DING Jin-ping et al (College of Agronomy,Guangxi University,Nanning,Guangxi 530003);Establishment and Optimization of SRAP Reaction Conditions for Arachis hypogaea L.[J];Journal of Anhui Agricultural Sciences;2008-10
4 DING Wei-Dong,CAO Li-Ping ,CAO Zhe-Ming Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences,Wuxi 214081,Jiangsu Province,China;The SRAP and SCAR molecular markers for detecting germ degeneration in Ctenopharyngodon idellus[J];Acta Zoologica Sinica;2008-03
5 ZHENG Yong-min,WANG Xing-fen,ZHANG Gui-yin,LI Xi-huan,MA Zhi-ying sources of Hebei, College of Agriculture, Agricultural University of Hebei, Baoding 071001, China);BAC library construction of Zhongmiansuo 12 with high yield, high quality and disease resistance[J];Journal of Agricultural University of Hebei;2004-03
6 WU Zhi-pu,YANG Wen-xiang,LIU Da-qun,ZHANG Ting (Department of Plant Pathology, Agricultural University of Hebei, Biological Control Center of Plant Pathogens and Plant Pests of Hebei Province, Baoding 071001, China);Establishment of SRAP technique system in wheat genome[J];Journal of Agricultural University of Hebei;2005-03
7 SU Yu-huan,WANG Jing-hua,LI Wen-qin,ZHANG Xiao-fang,XU jing,DUAN Hui-jun (Agricultural University of Hebei,Key Laboratory of Crop Germplasm Resources of Hebei,Baoding 071001,China);Main fruit traits and cluster analysis of watermelon germplasm[J];Journal of Agricultural University of Hebei;2008-06
8 WANG Zhi-yong1,2,YUAN Xue-jun2,LIU Jian-xiu2, GUO Hai-lin2(1.Department of Horticulture,Nanjing Agricultural Univeristy,Nanjing 210095, China;2.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China);Optimization for SRAP-PCR system in Cynodon dactylon and selection of primers[J];Acta Prataculturae Sinica;2008-03
9 CHEN Xuan1,GUO Hai-lin1,XUE Dan-dan2,ZHENG Yi-qi2,LIU Jian-xiu1(1.Institute of Botany,Jiangsu Province and Chinese Academy of Science,Nanjing 210014,China;2.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China);Identification of SRAP molecular markers linked to salt tolerance in Zoysia grasses[J];Acta Prataculturae Sinica;2009-02
10 ZHANG Tong, QU Shuping, CUI Chongshi (College of Horticulture, Northeast Agricultural University, Harbin 150030, China);Application of SRAP marker on genetics and breeding of vegetable crops[J];Journal of Northeast Agricultural University;2009-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WU Zhi-pu,YANG Wen-xiang,LIU Da-qun,ZHANG Ting (Department of Plant Pathology, Agricultural University of Hebei, Biological Control Center of Plant Pathogens and Plant Pests of Hebei Province, Baoding 071001, China);Establishment of SRAP technique system in wheat genome[J];Journal of Agricultural University of Hebei;2005-03
2 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
3 Li Li1 Zheng Xiaoying1* Liu Liwang2 1 Beijing Vegetable Research Center, Beijing Academy of Agriculture and Foresty Sciences, Beijing, 100097; 2 Nanjing Agricultural University Horticultural Institute, Nanjing, 210095;Analysis of Genetic Diversity and Identification of Cucumber Varieties by SRAP[J];Molecular Plant Breeding;2006-05
4 WANG Zhen-guo1,2,ZHANG Hai-ying2,YU Guang-jian1,ZHANG Feng2,WANG Yong-jian2,XU Yong2,GUO Shao-gui2,GONG Guo-yi2(1.College of Horticulture,Northeast Agricultural University,Haerbin 150030,China;2.Beijing Vegetable Research Center,Beijing 100097,China);The Orthogonal Optimization of SRAP Amplification System in Cucumber[J];Acta Agriculturae Boreali-Sinica;2007-04
5 Zuo Kaijing Sun Jizhong Zhang Xianlong Nie Yichun Liu Jinlan Feng Chunda(National Key Laboratory of Crop Genetics and Improvement, Huazhong Agricultural University, Wuhan 430070);Constructing a Linkage Map of Upland Cotton ( Gossypium hirsutum L.) Using RFLP, RAPD and SSR Markers[J];JOURNAL OF HUAZHONG AGRICULTURAL;2000-03
6 ZHAO Li-Ping1 LIU Li-Wang1,2* GONG Yi-Qin1 WANG Ming-Xia1 YU Fan-Min3 WANG Long-Zhi1(1.National Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095)(2.Department of Plant Sciences,North Dakota State University,Fargo,ND,USA 58105)(3.Shanghai Vegetable Sci & Tech Institute,Shanghai 201103);Cultivar Fingerprinting in Radish(Raphanus sativus) with SRAP and AFLP Marker[J];Bulletin of Botanical Research;2007-06
7 ZHANG Jun, WU Yao ting, GUO Wang zhen, ZHANG Tian zhen (Agronomy Department, Nanjing Agricultural University; Key Laboratory of Crop Germplasm and Breeding, Ministry of Agriculture, Nanjing 210095, China);Fast Screening of Microsatellite Markers in Cotton with PAGE/silver Staining[J];ACTA GOSSYPII SINICA;2000-05
8 Lei Jian,Liu Jun(Key Laboratory of Horticultural Plant Biology Huazhong Agricultural University,Ministry of Education;Potato Engineering and Technology Research Center of Hubei Province;Huazhong Agricultural University,Wuhan,Hubei 430070,China);Identification of a SRAP Marker Linked to Bacterial Wilt Resistance in Diploid Potato[J];Chinese Potato Journal;2006-03
9 WANG Yan~1,GONG Yi-qin~1,ZHAO Tong-min~2,LIU Guang~1,YU Fan-min~3,YE Hai-long~3,LIU Li-wang~(1*)(1.State Key Laboratory of Crop Genetics and Germplasm Enhancement/College of Horticulture,Nanjing AgriculturalUniversity,Nanjing 210095,China;2.Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;3.Shanghai Vegetable Science and Technology Extension Center,Shanghai 201105,China);Optimization of SRAP-PCR system and cultivar molecular identification in tomato(Lycopersicon esculentum L.)[J];Journal of Nanjing Agricultural University;2007-01
10 Ouyang Xinxing; Xu Yong;Zhang Haiying Kang; Guobin; Wang Yongjian (National Engineering Research Center for Vegetable, Beijing 100089 );Rapid Identification of Hybrid Purity in Watermelon Using Random Amplified Polymorphic DNA (RAPD)[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1999-01
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