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Potency Comparison of Recombinant Porcine Interferon-α by Two Kinds of Cells

WANG Xing-man TANG Ren-shu YU Han-yang ZHAO Jun WANG Ming-li (Department of Microbiology,Anhui Medical University,Key Laboratory of Gene Resource Utilization for Genetic Diseases,Ministry of Education and Anhui Province,Hefei Anhui 230032)  
Using vesicular stomatitis virus(VSV)as the attack virus and cytopathic effect inhibition method,the same batch with different concentrations of 8 groups of samples were rPoIFN-α potency tested.Taking standard recombinant human interferon-α as the potency titration reference standards which was specified by National Institute for the Control of Pharmaceutical and biological Products,the Madin-Darby bovine kidney(MDBK)cell and human laryngeal epitheilial cell(HEP-2)strains were compared with the results obtained.The rPoIFN-α antiviral potency of the sample under test cells in one-step and before adding cells until the cells covered with a single layer was compared with the sample under test cells together with the two-step method.The results showed that MDBK and HEp-2 for the rPoIFN-α antiviral activity of the test had no significant differences,which both could be used in rPoIFN-α antiviral activity detection.The titration of recombinant human interferon-α system could be used in rPoIFN-α potency test.The results of the two sampling method were basically the same,but one-step was recommended to use better.
【Fund】: 安徽省教育厅自然科学研究计划项目(编号:2004kj218);; 安徽省科技攻关计划重大科技专项(编号:08010302179)
【CateGory Index】: S852.4
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