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《Modern Agricultural Sciences and Technology》 2011-08
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Induction of Effect Factor on Mature Embryo Tissue Culture of Bothriochloa ischaemum

YU Na 1 DONG Kuan-hu 2 *(1 Department of Landscape,Shanxi Forestry Vocational Technical College,Taiyuan Shanxi 030009;2 College of Animal Science and Veterinary Medicine,Shanxi Agricultural University)  
Experiment was conducted on effect of different superficial sterilized measure,environmetal condition and subculture on callus induction of Bothriochloa ischaemum with its seeds as explants.The results showed that the explants sterilized way were immersed in 75% ethanol for 30s,then disinfested 0.1%(w∶v)HgCl2 solution for 15 min.Culture medium of callus induction was MSB+2.0 mg/L 2,4-D combined complete darkness culture that callus induction reached 86.7 %.Callus differentiation were improved evidence in the callus subculture medium MSB+1.0 mg/L 2,4-D.
【Fund】: 国家科技支撑计划项目(2007BAD56B01)
【CateGory Index】: S543.9
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