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《Journal of Anhui Agricultural University》 1989-01
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STUDIES ON THE EXTRACTS OF THREE RANUNCULACEAE SPECIES AND PROTOANEMONIN FOR BIOASSAYS

Wu Gongqian Zhang Chao (Agricultural College of Anhui) Wu Yuehuan (University of Science and Technology of China)  
From 1984 to 1987, 76 Samples of Amemone hnpehensis L. Ranunculus japonicus T. and Pulsatilla chinesis (Bunge) R. of Ranunculaceae plants were collected, Using water, human urine and alcohol as solvents, they were extracted by soak, distillation and so on. Bioassay results indicat that the extract is of very low insecticidal activity and very high in vitro fungistatic activity and low toxicity to fish.Sixteen protoanemonin and anemonin samples were extracted from the plants and synthesised, It was demonstratea that their structures accorded with the records in references by analysises of UV. IR. NMR. The results suggest that protanemonin is unstable in water and easy to polymerze, and the activities of the subsequent polymers are distinctly decreased while protoanemonin is stable in alcohol solution.
【Fund】: 中国科学院科学基金
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