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《JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY》 2002-01
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Construction of the Eukaryotic Expression Recombinant Plasmid Containing NDV-HN Gene

ZHONG Da lian 1,YU Wei yi 1,LIU Jing 2,LI Xiang ming 2,GUO Xiao Feng 3 (1.College of Animal Sciences and Fishery, Anhui Agricultural University, Hefei 230036; 2. School of Life Sciences,University of Science and Technology of China; 3.Departme  
A cDNA fragment of hemagglutinin neuraminidae (HN) was obtained from Neucastle disease virus (NDV) Lasota strain by reverse transcription (RT) PCR with a pair of designed primers.Its length was about 1 7 kb, which conformed to the NDV HN gene fragment's reported in the literature. The fragment was inserted into pcDNA3, an eukaryotic expression plasmid by a directional link. The constructed eukaryotic expression recombinant plasmid contained NDV HN gene was identified by an endonuclease and DNA sequencing.
【Fund】: 安徽省“十五”科技攻关项目 (0 1 0 1 3 0 0 3 )资助
【CateGory Index】: S852.4
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