Free Radical Scavenging Activity of Extracts from Fresh Leaves of Some Timber Trees in Anhui Province
HU Feng-lin (College of Forest Utilization, Anhui Agricultural University, Hefei 230036 )
A DPPH-Microplate assay was made to determine the free radical scavenging activity of the 80% methanol extracts from the leaves of some timber trees in Anhui Province. The results revealed that all trees have different free radical scavenging capacities, and as the raising of concentration and the prolonging of incubation time at 37 ℃, their free radical scavenging capacities increased. Of all trees, Pistasia chinensis Bunge, Acer nikoense Maxim., Acer buergerianum Miq., Liquidambar formosana Hance, Koelreuteria bipinnata Franch.var.integrifiola (Morr.) T., Picrasma quassioides (D.Don) Benn., Quercus acutissima Carr., Acer elegantulum Fang et Chiu, Acer davidii Franch., Elaeocarpus sylvestris (Lour.)Poir., Cyclobalanopsis glauca (Thunb.)Oerst., Acer henryi Pax, Fagus engleriana Seem., Sapium sebiferum (L.) Roxb., Koelreuteria Laxm.and Sorbus amabilis Cheng et Yu have the strongest free radical scavenging capacity.The extracts at a concentration that equals to 0.5 mg leaves per milliliter and after incubating for 20 min, their scavenging rates to 0.5 mmol·L~(-1) DPPH were 90.6%, 89.0%, 86.4%, 85.7%, 84.0%, 79.3%, 77.6%, 77.1%, 75.6%, 71.5%, 70.7%, 69.0%, 68.7%, 63.8%, 62.0% and 61.3%,respectively.