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《Journal of Anhui Agricultural University》 2005-03
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RAPD Analysis on Germplasm Resources in Genera Actinidia

JIA Bing,ZHU Li-wu,YU Xing,LU Li-juan,WANG Yan-fang(The Key Laboratory of Pomology,Anhui Agricultural University,Hefei 230036)  
The genomic DNA of 10 Actinidia species including 42 samples were extracted by the improved CTAB method. The parameters in RAPD-PCR were optimized. Thirty-eight arbitrary primers used in the study were screened from 80 ones. According to the data of RAPD-PCR,the cluster analysis was done by the SPSS (11.0) for Windows. The results showed that the relationship between A.chinensis and A.deliciosa was so close that they should be considered as the same species, and the classification between cultivars was accordant with their geographical distribution, and 18 characteristic bands between "Hayward"and "Wancui"were amplified in 14 primers by RAPD-PCR.
【CateGory Index】: S663.4
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