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《Journal of Anhui Agricultural University》 2006-03
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Establishment of AFLP technique system for Dangshansu pear and its variants determination

WANG Yan-fang,ZHU Li-wu,JIA Bing,ZHANG Shui-ming(Key Laboratory of Pomology,Anhui Agricultural University,Hefei 230036)  
The AFLP technique for pear genomic DNA analysis was established according to systematical study of the main factors involved in technique system.The digestion of genomic DNA was implemented by using two-step digestion with two restriction enzymes.500 ng DNA was digested with 5 units of EcoRⅠ and MseⅠ respectively in a reaction volume of 25 μL.Double-stranded adaptors were legated to the restriction fragments at 16℃ overnight(or 8~12 h).The digested-legated DNA fragment was diluted to 10 times with ddH_2O and used in pre-amplification.The pre-amplification products were diluted to 15 times with ddH_2O and used in selective amplification.The eight variants all have special bands in comparison with Dangshansu pear indicated that altered in genetics.The AFLP technique could be used to determine the mutants of pear trees.
【Fund】: 安徽省教育厅优秀人才计划项目资助
【CateGory Index】: S661.2
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【References】
Chinese Journal Full-text Database 3 Hits
1 ZHU Li -wu1,WANG Yan -fang1,JIA Bing1,ZHANG Shui -ming1,HUANG Yong -feng2,YANG Zhen -Jiang2(1Key Laboratory of Pomology,Anhui Agricultural University,Hefei,Anhui 230036 China;2Dangshansu Pear Germplasm Resources Protec-tion District Administration Committee,Dangshan,Anhui 235300 China);Study on the pear germplasm resources in Dangshan area,Anhui province by AFLP technology[J];Journal of Fruit Science;2009-02
2 SUN Wen-ying1,ZHANG Yu-xing1,LI Xiu-gen2,WANG Long2(1.College of Horticulture,Agricultural University of Hebei,Baoding 071001,China;2.Zhengzhou Fruit Institute,Chinese Academy of Agriculture Science,Zhengzhou 450009,China);Construction and Analysis of AFLP Molecular Linkage Map in Pear[J];Acta Agriculturae Boreali-Sinica;2009-03
3 CHENG Shui-ming,GAN Jian-ping,LIU Shi-wang,WANG Wei-xin(College of Life Science and Engineering,Huanggang Normal University,Huanggang 438000,Hubei, China);Segregation Pattern of AFLP Polymorphism Markers in Hybrid Progeny F_1 of Lentinula edodes[J];Hubei Agricultural Sciences;2009-12
China Proceedings of conference Full-text Database 1 Hits
1 CHENG Shui-Ming WANG Si-di ZEN Xiao-li Life Science and Engineering College,Huanggang Normal University,Huanggang 438000;Polymorphisms and segregation pattern of AFLP marks among hybrid F1 progeny in Lentinula edodes[A];[C];2008
【Citations】
Chinese Journal Full-text Database 8 Hits
1 ZHAO Hong, WANG Xing fen, ZHANG Gui yin, MA Zhi ying (College of Agrionomy, Agricultural University of Hebei,Baoding 071001,China);The study and establishment ofAFLP technical system in cotton[J];Journal of Agricultural University of Hebei;2002-01
2 WU Jun~(1,3),SHU Huai-rui~1,ZHANG Kai-chun~(2*),ZHANG Xue-ning~2,JIANG Li-jie~2 (1 College of Horticulture,Shandong Agriculture University,Taian,Shandong 271018,China;2 Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,China;3 College of Horticulture,Nanjing Agriculture University,Nanjing 210095,China);Optimized AFLP System and Study of Cluster Analysis for Peach[J];Acta Botanica Boreali-occidentalia Sinica;2005-03
3 Zhu Jun Wang Tao Li Guangchen Zhao Yujun Zhang Wen Zhou Aiqin(College of Horticulture,CAU);Construction of AFLP Analysis System in Apple[J];JOURNAL OF CHINA AGRICULTURAL UNIVERSITY;2000-03
4 Chen Yihua, Jia Jianhang, Li Chuanyou, Jin Demin, Weng Manli, Wang Bin (Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 );Rice Seed Identification by Computerized AFLP-DNA Fingerprinting Analysis[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;2000-03
5 WU Min-Sheng, WANG Shou-Cai, DAI Jing-Rui (Department of Genetic and Breeding, China Agricultural University, Beijing 100094);Application of AFLP Markers to Heterotic Grouping of Elite Maize Inbred Lines[J];Acta Agronomica Sinica;2000-01
6 CHEN Wan-Quan; QI Xiao-Quan; RE. Niks (Department of Plant Breeding Wageningen Agricultural University, NL 6700 AJ, Wageningen, The Netherlands)(Institute of Plant Protection CAAS Beijing 100094);Localization of Genes for Partial Resistance to Puccinia hordei at the Seedling Stage in Barley by Using AFLP Markers[J];ACTA GENETICA SINICA;1999-06
7 Weng Yuejin(Institute of Crop Germplasm Resources, CAAS, Beijing 100081)Santosh GurtuS N Ninam(ICRISAT, Pantancheru 502 324,India);Fingerprinting in groundnut using AFLP[J];CHINESE JOURNAL OF OIL CROP SCIEVES;1999-01
8 Luo Ancai 1,2 ,Li Chunfan 1 ,Huang renhu 3 ,Li Daogao 1 ( 1 College of Hort iculture and Landscape Architecture,Southwest Agriculture University,Chong qing 400716; 2 Biological Department,Chongqing Normal University,Chongqing 400043; 3 Fengjie Agric ultural Bureau, Chongqing 404600);Analysis of the Mutants of Fengjie Navel Orange(Citrus Sinensis Osbeck)by AFLP[J];Chinese Agricultural Science Bulletin;2003-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 JIANG Xian-rong,YANG Jie,ZHOU Yan-mao(Department of Biology,Hefei Teachers College,Hefei 230061,China);Advances in the Application of Transgenic Technology and the Safety of Transgenic Organisms[J];Journal of Hefei Teachers College;2008-03
2 Ding Jiangang1 Hu Dinghui2(1College of Life Science and Engineering SWUST,MianYang,SiChuan 621010,China;2College of Life Science and Technol-ogy MNU);Studies on Genetic Transformation System of Lettuce Mediated by Agrobacterium Tumefacie[J];Anhui Agricultural Science Bulletin;2008-04
3 Huang Yan1 Guo Qingshui1 Xu Lixin1* Chen Shoucai2(1 College of Life science and Agriculture of Hainan University,Haikou 570228;2 Chinese Academy of Tropical Agriculture Science,Danzhou 571101);Comparision of three methods of Genomic DNA Extraction from rubber tree[J];Anhui Agricultural Science Bulletin;2008-07
4 Cai Jian et al (Key Laboratory of Rice Genetics and Breeding of MOA, Hefei 230031);Study Progress of the Relationship between Heterosis and Genetic Differentiation of Crossing Parents in Crop[J];Journal of Anhui Agricultural Sciences;2002-04
5 Huang Min et al (College of Life Science,Yangtze University, Jingzhou,Hubei 434025);Transformation Research Progress in Maize[J];Journal of Anhui Agricultural Sciences;2004-05
6 CHANG Xi-yun et al (Northwest Plateau Institute of Biology,the Chinese Academy of Sciences,Xining,Qinghai 810001);Survey of the Wheat Variety with the Resistance to Stripe Rust Disease[J];Journal of Anhui Agricultural Sciences;2005-09
7 AN Yun-hua(College of Gardening and Horticulture,Yangtze University,Jingzhou,Hubei 434025);Research Progress of Gene Engineering of Orchid[J];Journal of Anhui Agricultural Sciences;2006-01
8 ZHANG Shao-bin et al (College of Biological Science and Technology, Shenyang Agricultural University, Shenyang,Liaoning 110161);Protein Engineering of Crop Resistance to Insects and Its Application[J];Journal of Anhui Agricultural Sciences;2006-08
9 LIU Guan-ming et al (Agriculture and Garden College,Zhongkai Agrotechnical University,Guangzhou,Guangdong 510225);SSR Analysis of Genetic Diversity of Shanyou and Yueyou Peanut Cultivars[J];Journal of Anhui Agricultural Sciences;2006-11
10 CHEN Xing-jiang et al (Guizhou Tobacco Institute,Guiyang,Guizhou 550003);Dormancy Gene SPS from Arabidopsis and its Construction of Plant Expression Vector[J];Journal of Anhui Agricultural Sciences;2006-15
China Proceedings of conference Full-text Database 10 Hits
1 FAN Rong-hui~(1,2,3) HUANG Min-ling~(1,2,3) ZHONG Huai-qin~(1,2,3) WU Jian-shen~(1,2,3) LIN Bing~(1,2,3) YE Xiu-xian~(1,2,3) LUO Yuan-hua~(1,2,3) (1 Institute of Crop Sciences,Fujian Academy of Agricultural Science,Fuzhou 350013; 2 Flowers Research Center,Fujian Academy of Agricultural Science,Fuzhou 350013; 3 Fujian Engineering Research Center for Characteristic Floriculture,Fuzhou 350013);Cloning and Expression Analysis of Key Enzyme Genes in Blue Flowers from Strelitzia reginae[A];[C];2011
2 PENG Wentao,WANG Feng,CUI Zhongli,CAO Hui (Key Laboratory of Microbiological Engineering of Agricultural Environment,MOA, Nanjing Agricultural University,Nanjing,210095);The impacts of transgenic cotton on soil microbes' kanamycin resistance[A];[C];2009
3 Wang yuan-dong~1;Chert shao-jiang~2;Duan min-xiao~1;Zhang chun-yuan~1;Guo jing-lun~1;Zhao jiu-ran~1(1. Maize Research Center, BeijingAcademy of Agricultural & Forestry Sciences 100089 2. National Maize Improvement Center 100094);P Germplasm lines for China Temperate Maize Germplasm Development[A];[C];2004
4 LI Yu-ling~1;WANG Yan-zhao~1;LU De-bin~1;CHEN Shao-jiang~2;TANG Ji-hua~1 (1. Agronomy College of Henan Agricultural University, Zhengzhou 450002, China; 2. National Maize Improvement Center of China, China Agricultural University, Beijing 100094, China);The Genetic Relationship of Popcorn with Normal Corn Inbreds and Heterosis Predication[A];[C];2004
5 ZHANG Ren-he, XUE Ji-quan, MAO Jian-chang, Ma Guo-sheng, YANG Yin-fu (College of Agronomy Northwest Science and Technology University of Agriculture and Forestry, Yangling, Shaanxi, 712100, China);Study on Genetic Markers and Germplasm Resoures in Maize[A];[C];2004
6 ZHANG Hong-mei WANG Fu-rong YANG Shu-cheng LUO Qi Maize Research Institute,Shanxi Academy of Agricultural Science,Shanxi Xinzhou 034000,China; ZHANG Hong-wei TAN Zhen-bo Beijing DBN Institute of Agricultural Sci & Tech,Beijing 100085,China; LIU Xiao-hong ZHENG Zu-ping Maize Research Institute,Sichuan Agricultural University,Sichuan Ya'an 625014,China;QTL mapping for maize resistance to MDMV in a recombinant inbred lines[A];[C];2005
7 LIU Wei~(1,2) LI Zi-chao~2 SHI Yan-li~1 MA Hong-wen~1 WANG Jian~1 ZHANG Hong-liang~2 1.The Crop Research Institute of Ningxia Academy of Agricultural and Forestry Sciences,Ningxia Yongning 750105,China; 2.Department of Plant Genetics and Breeding,China Agricultural University,Beijng 100094,China;Genetic diversity of Japonica rice varieties based on SSR markers[A];[C];2005
8 Wang Qiang, Zhang Maojun ( Pomology Research Institute Jilin Academy of Agricultural Sciences,Gongzhuling Jilin 136100, China);Application of Molecular Marker Technology to Heredity and Breeding of Malus Mill.[A];[C];2004
9 ;农杆菌介导花生白藜芦醇合酶基因转化马铃薯的研究[A];[C];2006
10 ;Study on a genetic transformation system in Dendrathema grandiflorum‘Lv Ying Ge’[A];[C];2009
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Luo Haiyan et al.(1 Tropical Crops Genetic Resources Institutes,Chinese Academy of Tropical Agriculture Sciences,Hainan 571737,China;2 Chinese Academy of Tropical Agriculture Sciences,Hainan 571737,China);ISSR Molecular Marker and its Using[J];Anhui Agricultural Science Bulletin;2008-19
2 Cai Jian et al (Key Laboratory of Rice Genetics and Breeding of MOA, AAAS, Hefei 230031);Application of AFLP Molecular Marker in Crop Breeding[J];Journal of Anhui Agricultural Sciences;2002-02
3 Li Miao et al (Department of Plant Protection,Anhui Agricultural University,Hefei 230036);Study on the Leaf Tissue Structure of Kiwifruit Cultivars in Relation to Bacterial Canker Disease Resistance[J];Journal of Anhui Agricultural Sciences;2002-05
4 WANG Yan1,LUO Wenqian2,ZHANG Zhiqi1(1.School of Chemistry & Material Science,Shaanxi Normal University,Xi'an710062,Shaanxi,China;2.Department of Chemistry & Chemical Engineering,Ankang University,Ankang725000,Shaanxi,China);Response Surface Method and Its Application in Natural Product Component Extraction[J];Journal of Ankang University;2009-01
5 SONG Feng-shun~1,ZHU Li-wu~1,ZHANG Shui-ming~1,JIA Bing~1,YANG Zhen-jiang~2,HUANG Yong-feng~2 (1.Department of Horticulture,Anhui Agricultural University, Hefei, 230036; 2 Administration Committee,Protection District of Dangshansu Pear Germplasm Resources,Dangshan,235300);RAPD Analysis of the Pear Germplasm Resources in Protection District of Dangshansu Pear[J];Journal of Anhui Agricultural University;2004-03
6 Gao jiangsheng 1 ,Zhang Weifang 1 ,Ou Yonghui 2 ,Yang Pengwei 3 (1The College of Plant Science and Technology,Tarim Agricultural Reclamation University,Alar Xinjiang843300,China;2Prefecture36,Agri.Division2,Xinjiang Production and Construction Group,Ruoqiang Xinjiang841000,China;3Prefecture65,Agri.Division4,Xinjiang Production and Construction Group,Yining Xinjiang835200,China);Effects on the fast propagation by cultural tissue of pear variety-Xinli No.7[J];Northern Fruits;2003-03
7 Li Rong,Niu Jian-xin(Agricultural College of Shihezi University,Shihezi,Xinjiang 832003,China);AFLP molecular marker technique and its application in the studies of fruit trees[J];Northern Fruits;2006-05
8 XIE Ji-rong1,2,LIANG Guo-lu2,LI Shu-fa3,CHENG Zai-quan3,TANG Kai-xue 3,HUANG Xing-qi3 (1.Department of Life Science,Chongqing University of Arts and Sciences,Chongqing 402168,China;2.College of Horticulture and Gardens,Southwest University,Chongqing 400715,China;3.Yunnan Academy of Agricultural Sciences,Kunming 650223,China);Identification of Pink Sprout Mutation from 'Gold Island' Rose[J];Northern Horticulture;2007-11
9 Yang Chaodong 1,2 Zhang Junwei 1 Xiong Caifeng 3 Bao Manzhu 1 (1 College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070,Wuhan,P.R.China 2 Xiaogan University, 432100,Xiaogan,P.R.China 3 China Mei Flower Research Center,430074,Wuhan,P.R.China);Rapid Identification of Parentage in Prunus mume by AFLP Assay[J];Journal of Beijing Forestry University;2004-S1
10 XU Hong-Mei,QIN Ling(Beijing Agricultural College, Beijing 102206);The AFLP and Its Application in Fruit Tree[J];Journal of Beijing Agricultural College;2003-04
【Secondary References】
Chinese Journal Full-text Database 6 Hits
1 LI Xiu-gen,YANG Jian,WANG Long,WANG Su-ke (Zhengzhou Fruit Research Institute,CAAS,Zhengzhou,Henan 450009 China);Progress and proposal in research of pear breeding in China[J];Journal of Fruit Science;2010-06
2 YIN Hao,WU Jun,ZHANG Shao-ling (Pear Engineering and Technology Research Center,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China);Construction of molecular genetic maps and location of important agronomic traits in pear[J];Journal of Fruit Science;2012-05
3 ZHU Rui-rui 1,GAO Yi-ke1,ZHANG Qi-xiang1,2(1.Department of Ornamental Horticulture,College of Landscape Architecture,Beijing Forestry University,Beijing 100083,China;2.China National Engineering Research Center for Floriculture,Beijing 100083,China);Optimization of AFLP of Aquilegia[J];Acta Agriculturae Boreali-Sinica;2010-S1
4 LI Xiao-feng,LI Xue,JIA Bing,LIU Li,YE Zhen-feng,HENG Wei,and ZHU Li-wu (Key Laboratory of Pomology,Anhui Agricultural University,Hefei 230036,China);Analysis of Enzyme Activity and Lignin Content and Expression of CCoAOMT Gene in the Pericarp of‘Dangshan Suli’and Its Russet Mutant[J];Acta Horticulturae Sinica;2012-05
5 Cheng Xiaomao,Yue Lin,Huang Xiaoxia (College of Landscape and Architecture,Southwest Forestry University,Kunming 650224);Analysis on the Genetic Relationship of Acer trialum L.and Acer buergerianum Miq.by AFLP[J];Chinese Agricultural Science Bulletin;2011-16
6 YAO Qiang1,LIU Yan2,GONG Zhi-yuan1,GAO Xing-xi3,HAN Jian-dong1,REN Peng-fei1, WAN Lu-zhang1,REN Hai-xia1,ZHANG Xue-mei2,LI Jin1,QU Ling1(1.Institute of Agricultural Resources and Environment,Shandong Acdemy of Agricultral Scieneces,Ji'nan Shandong 250100; 2.School of Life Sciences,Qingdao Agricultural University,Qingdao Shandong 266109; 3.Institute of Mycological Science and Techonolgy,Ludong University,Yantai Shandong 264025);Advances on Molecular Biological Study of Lentinus edodes[J];Edible Fungi of China;2011-06
China Proceedings of conference Full-text Database 1 Hits
1 YAO Qiang~1 LIU Yan~2 GONG Zhi-Yuan~(1*) GAO Xing-Xi~3 HAN Jian-Dong~1 REN Peng-Fei~1 WAN Lu-Zhang~1 REN Hai-Xia~1 1 Institute of Agricultural Resources and Environment,Shandong Acdemy of Agricultral Scieneces,Shandong Ji'nan 250100 2 School of Life Sciences,Qingdao Agricultural University,Shandong Qingdao 266109 3 Institute of Mycological Science and Techonolgy,Ludong University,Shandong Yantai 264025;Application of Molecular Biological Techniques in Study of Lentinus edodes[A];[C];2011
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 Zhou Yihua Chen Zhenghua (Institute of Genetics,The Chinese Academy of Sciences Beijing 100101);DEVELOPMENT AND APPLICATION OF MOLECULAR MARKERS IN BOTANY[J];JOURNAL OF WUHAN BOTANICAL RESEARCH;1999-01
2 YUAN Li-Xing, FU Jun-Hua, Warburton M, LI Xin-Hai, ZHANG Shi-Huang, Khairallah M2, LIU Xin-Zhi, PENG Ze-Bin, LI Lian-Cheng ( Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Crop Genetics and Breeding, Ministry of Agr;Comparison of Genetic Diversity among Maize Inbred Lines Based on RFLPs, SSRs, AFLPs and RAPDs[J];ACTA GENETICA SINICA;2000-08
3 Luo Ancai, Li Daogao, and Yang Xiaohong ( Department of Horticulture, Southwest Agricultural University, Chongqing 400716);Advances of the Study on the Metabolism of Organic Acids in Citrus Fruit[J];Acta Horticulturae Sinica;2001-S1
4 LUO An-cai 1,2 , YANG Xiao-hong 1, DENG Ying-yi 1, LI Chun-fan 1, XIANG Ke-shu 3, LI Dao-gao 1 ( 1College of Horticulture and Landscape Architecture, Southwest Agricultural University, Chongqing 400716; 2Biological Department, Chongqing Normal University, Chongqing 400043; 3Fengjie Agricultural Bureau of Chongqing, Fengjie 404600);Organic Acid Concentrations and the Relative Enzymatic Changes During the Development of Citrus Fruits[J];Scientia Agricultura Sinica;2003-08
5 Jia Jizeng[WT〗 (Institute of Crrop Germplasm Resources, CAAS, Beijing 100081);Molecular Germplasm Diagnostics and Molecular Marker assisted Breeding[J];SCIENTIA AGRICUTURA SINICA;1996-04
6 1WANG Bin 1LI Chuan You 1,3 ZHENG Hong Gang 1FU Jian Min 2YANG Ren Cui 3Henry T. NGUYEN 1(Institute of Genetics,The Chinese Academy of Sciences, Beijing 100101) 2(Institute of Genetics and;Primary Study of Rice AFLP Analysis Optimization of Reaction Conditions and Analysis of Thermo sensitive Genic Male Sterile Rice Allelic Mutant Lines [J];ACTA BOTANICA SINICA;1999-05
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