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《Journal of Anhui Agricultural University》 2008-01
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Genetic diversity of Iris lactea determined by AFLP marker

MU Shao-hua1,2,PENG Zhen-hua2,QIE Guang-fa2,SUN Zhen-yuan2,SUN Qi-xiang2(1.International Center for Bamboo and Rattan,Beijing 100102;2.Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091)  
The AFLP(amplified fragment length polymorphism) technique was used to analyze genetic diversity of 10 different populations of Iris lactea collected from China.Genomic DNA was digested with EcoRⅠ and MseⅠ enzymes and amplified with 18 pairs of EcoRⅠ+3/MesⅠ+3 Primers combinations.AFLP analysis produced 1164 scoreable bands,of which 752(65.11%) were polymorphic.Cluster Analysis was based on the UPGMA method.Populations in Changchun and Zhuozhou city showed a greater genetic variability and were separately classified into two groups.Populations in Guyuan,Minqin,Wuwei,Beijing and Taishan were classified into one group,and those in Taipu,Xiwuqi and Wulumuqi were in another group.The genetic relationships among the populations were related to their locations,especially to the latitude.
【Key Words】： Iris lactea AFLP genetic diversity polymorphism
【Fund】： 国家重大科技基础性工作专项(2002DEAl0009)资助
【CateGory Index】： S682.19
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