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《Journal of Anhui Agricultural University》 2008-03
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Extraction of genomic DNA and optimization of RAPD technique system in melon

YU Ming-hui1,XU Liu-jun2,LIU Tong-guang1,CHEN Yun-dong1,SONG Xiao-yan1 (1.School of Horticulture,Anhui Agricultural University,Hefei 230036;2.Popularization Centre of Agricultural Technique,Xuanzhou Agricultural Committee,Xuancheng 242000)  
The changes of extraction method by CTAB have influence upon the quality and yield of the genomic DNA in melon.The orthogonal experiment L9(33) was made to investigate efficiency of the RAPD-PCR in different conditions,the optimal combination of ingredient in RAPD-PCR was established.The result of repeated experiment showed that the established system was stable and reliable.
【CateGory Index】: S652
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