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《Journal of Anhui Agricultural University》 2008-03
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Extraction of genomic DNA and optimization of RAPD technique system in melon

YU Ming-hui1,XU Liu-jun2,LIU Tong-guang1,CHEN Yun-dong1,SONG Xiao-yan1 (1.School of Horticulture,Anhui Agricultural University,Hefei 230036;2.Popularization Centre of Agricultural Technique,Xuanzhou Agricultural Committee,Xuancheng 242000)  
The changes of extraction method by CTAB have influence upon the quality and yield of the genomic DNA in melon.The orthogonal experiment L9(33) was made to investigate efficiency of the RAPD-PCR in different conditions,the optimal combination of ingredient in RAPD-PCR was established.The result of repeated experiment showed that the established system was stable and reliable.
【CateGory Index】: S652
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Chinese Journal Full-text Database 8 Hits
1 ZHANG Dong xiao 1, A. NUKAYA 2 (1.Department of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2.Faculty of Agriculture, Shizuoka University, Shizuoka 422, Japan);Comparison among some cultivars or lines of western melon by using RAPD analysis[J];Journal of Fujian Agricultural University(Natural Science);2000-04
2 WANG Jianshe1,MENG Shuchun1,SONG Shuhui1,CHEN Guilin2,SUN Zhenying2,LI Mei2(1.Beijing Vegetable Research Center,Beijing100089,China;2.Hebei Agricultural University,Baoding071001,China);Molecular Marker′s Polymorphism Between Mapping Parents on Cucumis melo L.[J];Acta Agriculturae Boreall—sinica;2002-04
3 Wang Zhangjun~(1,2),Wang Jianshe~1,Liu Shengxiang~2,Xu Xing~2(1.National Engineering Research Center for Vegetable,Beijing 10089,China;2.School of Agriculture,Ningxia University,Yinchuan 750021,China);Analysis of random amplified polymorphic DNA marker in melon(Cucumis melo L.)[J];Journal of Agricultural Sciences;2006-01
4 Yao Guoxin1 Wang Jianshe2 Yongqiang3 Chen Susheng1(1 Ningxia University, Yinchuan Ningxia 750021;2 National Engineering Research Center for Vegetables,Beijing 100089; 3 Capital Normal University, Beijing 100037);Polymorphism of Fragment Length of Random Primers Produces of Mapping Parents of Melon(Cucumis melo.)[J];Journal of Capital Normal University;2005-01
5 Wei Huijun Du Shengli Ma Dehua Tian jin's Cucumber Research Institute, Tianjin 300192;Application of Molecular Marker in the Study on Cucumber's Genetics and Breeding[J];BIOTECHNOLOGY INFORMATION;1999-02
6 ZHANG Jian-nong,ZHAO Jian-hua,LI Ji-hong,CAO Zi-yi (G'ansu agricultural university ,Lanzhou 730070,China);Genetic relationship analysis of melon by RAPD marker[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry;2005-12
7 GAO Lan,LI HaoMing(College of Science & Technology of Qingdao University,Qingdao,Shandong 266071,China);The Application of DNA Molecular Marker on Tomato Breeding[J];Hereditas(Beijing);2003-03
8 MENG Xiangdong; MA Hong & GAI Shupeng(Departmens of Horticulture, Shandong Agricultural University, Taian, Shandong 271018);RAPID EXTRACTION METHODS OF VEGETABLE DNA SUITABLE FOR PCR ANALYSIS[J];CHINESE JOURNAL OF APPLIED AND ENVIRONMENTAL BIOLOGY;1998-03
Chinese Journal Full-text Database 10 Hits
1 HU Zhong-yi et al (Guizhou Tobacco Research Institute,Guiyang,Guizhou 550003);Application and Prospect of Molecular Markers in Tobacco Breeding[J];Journal of Anhui Agricultural Sciences;2007-25
2 XIE Li-bo et al(Sub-branch of Horticulture,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150069);Research on the Establishment of RAPD Amplification Program of Hot Pepper[J];Journal of Anhui Agricultural Sciences;2010-34
3 HAN Cui-xuan et al (College of Bioscience and Bioengineering,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018);Research Progress on Molecular Marker for Resistance Genes to Tomato Yellow Leaf Curl Disease[J];Journal of Anhui Agricultural Sciences;2011-02
4 ZHAO Chun-mei et al(Harbin Academy of Agricultural Science,Harbin,Heilongjiang 150070);Genetic Diversity Analysis of Cucumis melo L. Based on Inter Simple Sequence Repeat[J];Journal of Anhui Agricultural Sciences;2012-01
5 ZHU Yong-ping 1 TIAN Lu 2 WU Yun-yun 2 HE Feng-mei 2 YANG Xiao-hong 3(1 College of Agriculture and Biotechnology,Yunnan Agricultural University,Kunming Yunnan 650201;2 College of Horticulture and Landscape,Yunnan Agricultural University;3 College of life Science and Technology,Kunming University);Comparison Research of Extraction Method of Total RNA from Labellum of Cymbidium Sinense[J];Modern Agricultural Sciences and Technology;2010-14
6 SONG Feng-shun~1,ZHU Li-wu~1,ZHANG Shui-ming~1,JIA Bing~1,YANG Zhen-jiang~2,HUANG Yong-feng~2 (1.Department of Horticulture,Anhui Agricultural University, Hefei, 230036; 2 Administration Committee,Protection District of Dangshansu Pear Germplasm Resources,Dangshan,235300);RAPD Analysis of the Pear Germplasm Resources in Protection District of Dangshansu Pear[J];Journal of Anhui Agricultural University;2004-03
7 CHU Xiao hong 1, YANG Gong she 1, ZHENG Xin ming 2 (1.College of Animal Science and Veterinary Medicine,Northwest Science and Technology University of Agriculture and Forestry,Yangling,Shaanxi,712100,China;2.Centre of Biotechnology in Agricultu;Gene Targeting and Its Affecting Factors[J];Journal of Yellow Cattle Science;2001-05
8 FAN Jinxia,QIN Zhiwei(Horticulture College,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Study progress on DNA molecule makers application in Cucumis for genetic breeding[J];Journal of Northeast Agricultural University;2006-02
9 ZHANG Xiaoxuan,LI Jingfu,XU Xiangyang(Horticulture College,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);RAPD molecular marker method and its application to tomato researches[J];Journal of Northeast Agricultural University;2006-03
10 GAO Yongli,LI Jingfu,WANG Aoxue,XU Xiangyang(College of Horticulture,Northeast Agricultural University,Harbin 150030,China);Optimalization of RAPD reaction system in tomato[J];Journal of Northeast Agricultural University;2007-02
China Proceedings of conference Full-text Database 1 Hits
1 LI Wei-hua~(1,2) ZHANG Tian-zhen~1 (1.State Key Laboratory of Crop Genetics & Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095; 2.Xuzhou Agricultural Institute,Xuzhou 221121,China);Anti-insect Research by Transgenic Methods in Upland Cotton:Present Status,Problem and Prospect[A];[C];2008
Chinese Journal Full-text Database 10 Hits
1 YU Zhongwei,XIANG Changping (College of Horticulture and Forest, Huazhong Agricultural University,Wuhan 430070);Analysis of Heterosis on Agronomic Character of Bitter Melon[J];Journal of Changjiang Vegetables;2008-14
2 WANG Wei-hua 1 ,YU Guo-ping 1 ,ZHANG Li-dong 2(1. Food College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC2. Command and Engineering College of Chemical Defense People’s Libration Army, Beijing 102205, PRC );The initial research about extractive condition of lycopene[J];Journal of Northeast Agricultural University;2004-05
3 LIU Zheng guo (Agric. Colloge,Guangxi Univ.,Nanning 530005,China);Studies on heterosis and combining ability of yield in bitter gourd (Momordica charantica L.)[J];Journal of Guangxi Agricultured and Biological Science;2002-04
4 LIU Zhengguo~1,XIAO Xi(1 Agric.College,Guangxi Univ.,Nanning 530005,China; 2 Nanning Kenong Seedling Limited Corporation,Nanning 530007,China);Studies of combining ability and genetic correlation for economic characters in bitter gourd ( Momordica charantia L.)[J];Journal of Guangxi Agricultural and Biological Science;2004-01
5 LIU Zheng guo 1,2 , LONG Ming hua 2 , QIN Rong yao 2, WANG Xian yu 2 ( 1 College of Horticulture and Gardening, Hunan Agricultural University , Changsha 410128, China; 2 Agricultural College, Guangxi University , Nanning 530005, China );Studies on genetic variation,correlation and path analysis in bitter gourd ( Momordica charantia L.)[J];Guihaia;2005-05
6 Li Dazhong Wen Qingfang Kang Jianban Li Yongping Xue Zhuzheng (Research Center of Vegetable Science,Fujian Academy of Agricultural Science,Fuzhou,350013);Correlation and Path Analysis of Main Agronomic Traits of Balsam Pear[J];Journal of South China University of Tropical Agriculture;2007-03
7 Xiang Changping 1) Xie Jun 2) Nie Qijun 1) Wang Liping 1) ( 1) Huazhong Agricultural University, Wuhan 430070, China; 2) Zhongshan University, Guangzhou 510275, China);Principal Component Analysis on Characters of Balsam Pear[J];Journal of Huazhong Agricultural;2001-04
8 NIE Ji-yun,L I Jing,MA Zhi-yong,ZHANG Gui-fen,L I Ming-qiang (supervision & inspection center of fruit & nursery stocks quality of the ministry of agriculture,xingcheng 1 2 5 1 0 0 );Program designation of grey relation analysis and an example of its usage[J];COMPUTER AND AGRICULTURE;2000-04
9 WAN Xin-jian, CHEN Xue-jun, MIAO Nan-sheng, FANG Rong (Institute of Vegetable and Flower, Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China);Present Situation and Prospect of Studies on Momordica charantia in China[J];Acta Agriculturae Jiangxi;2002-03
10 DENG huan1,LIU tong-guang1,DAI zu-yun2,YANG pei-zhu2,SUN ming-wu2,SONG Xiao-yan1,CAI Xu1(1.School of Horticulture,Anhui Agricultural University,Hefei 230036;2.Hefei Jianghuai Horticultural Institute,Hefei 230038);Combining ability analysis based on quantitative characters in melon[J];Bulletin of Agricultural Science and Technology;2009-01
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 HUANG Ya-jie1,YUN Guang-rong1,LI Mei1,LI Chang-zeng2,XIAO Yu1,ZHANG Bin1(1Tianjin Kernal Vegetable Research Institute,Tianjin 300384,China;2Agriculture and Animal Husbandry Bureau of Yutian County,Yutian 064100,Hebei,China);Research Progress on Genetic Breeding of Bitter Gourd(Momordica charantia L.)[J];China Vegetables;2012-08
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Cai Jian et al (Key Laboratory of Rice Genetics and Breeding of MOA, AAAS, Hefei 230031);Application of AFLP Molecular Marker in Crop Breeding[J];Journal of Anhui Agricultural Sciences;2002-02
2 WANG Yong\|Qing\ XU Lai\|Xiang\ ZHANG Zhi\|Bin (National Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, The Chinese Academy of Sciences Beijing\ 100080,China);The Standardization of RAPD[J];CHINESE JOURNAL OF ZOOLOGY;2000-04
3 GAO Lan 1,FU Jian xi 1,WANG Jian hua 2,WANG Rong 2(1 College of Life Science,Northwest Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Horticulture Institute of Xian,Lintong,Shaanxi 710603,China);RAPD analysis of tomato F_1 hybrid seeds[J];Acta Botanica Boreali-occidentalia Sinica;2001-03
4 Guo Suzhi He Chengkun Li Jiashen Cai Tianqing(Dept. of Horticulture, FAC, Fuzhou 350002);Peroxidase Isozyme in Cucumis melo L. and Its Fuzzy Cluster Analysis[J];;1992-03
5 ZHAO Ling xia 1,2 , LI Jing fu 2, TANG Ke xuan 1, XU Xiang yang 2, KAI Guo yin 1,3 (1.State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China;2.College of Horticulture, North East Agricultural University, H;Genetic diversity analysis of Lycopersicon by RAPD markers[J];Journal of Fujian Agricultural University(Natural Science);2002-02
6 Xie Chuanxiao , Zhu Suwen , Li Peijin , Chen Beijiu , Yu Zengliang ( School of Life Science, Anhui Agricultural University, Hefei 230036) ( Key Laboratory of Bioengineering, Chinese Academy of Sciences, Hefe;Obtaining of SCAR Markers of Two Dominant Genes for Opposite Leaves and Fruits Trait of Zea Mays[J];High Technology Letters;2002-08
7 Zhang Xingping Wang Ming (Northwestern Agricultural University);ISOZYME ANALYSIS OF HYBRIDS AND THEIR PARENTS ON WATERMELON [Citrullus lanatus (Thanb.)Mansf.][J];;1989-02
8 YueJinhua, Shi Jiang, Zhao Huji, and Zhu Xinxia (Department of Biology Science, Xinjiang Shihezi University,Shihezi,832003);Study on the Kinship and Purity of Rock-melon by RAPD Analysis[J];;2000-04
9 MENG Xue ping,YANG Heng shan,HOU Li bai,ZHOU Li bo, (1.Inner Mongolia University for the Nationalities,Tongliao Inner Mongolia 028043,China; 2.Shenyang Agricultural University,Shenyang 110161,China);Study on Peroxidase Isoenzymes of Winter Wheat in Different Development Stages[J];Acta Agriculturae Boreall—sinica;2001-03
10 Cao Wanhong; Zhao Yanru(Beijing Vegetable Research Center, Beipng 100081);An Analysis on Isozyme and Soluble Protein in Watermelon[J];ACTA AGRICULTURAE BOREALL—SINICA;1994-02
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