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《Journal of Anhui Agricultural University》 2008-03
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Research on RAPD marker to SCAR marker in Camellia sinensis

DING Zhou1,JIANG Chang-jun2,YE Ai-hua1,CHEN Cong1,TAN Zhen1,ZHANG Li1(1.School of Biology Science,Anhui Agricultural University,Hefei 230036;2.School of Tea and Food Science,Anhui Agricultural University,Hefei 230036)  
SCAR marker is one of new molecular markers based on RAPD technology.It has such merit as low price,high stability,site-specific and so on compared with other markers,and it may provide a new way for molecular breeding of tea.In this experiment,the special segment with random primer in different tea cultivars genomic DNA was recovered,cloned and sequenced.According to its sequence,a pair of special primers was synthesized and the RAPD marker was successfully converted into SCAR marker.It improves stability of molecular identification and affirm applied prospect of SCAR technology in genetic resources of tea.
【Key Words】： tea plant(Camellia sinensis) RAPD marker SCAR marker convert
【Fund】： 安徽省教育厅自然科学研究计划重点项目(2004kj137zd)资助
【CateGory Index】： S571.1
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