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《Journal of Anhui Agricultural University》 2009-04
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Molecular identification of Macrolepiota sp. H-603 and optimization of solid culture

FENG Fei1,HOU Cheng-lin1,QIN Dao-chun2 (1.College of Life Science,Capital Normal University,Beijing 100048;2.SFA Key Laboratory on Bamboo and Rattan Science and Technology,International Center for Bamboo and Rattan,Beijing 100102)  
In this paper,the ITS region of nuclear ribosomal DNA of Macrolepiota sp. was sequenced,and a phylogenetic tree was set up based on ITS data.The solid culture method was selected to study the growth state of Macrolepiota sp.in different media and culture conditions.The results showed that the phylogenetic relationships of Macrolepiota sp.were closely related to Macrolepiota sp. Oku X-2000 and Macrolepiota sp. TMI-23928.The results of orthogonal experiment showed that C source,pH value,mineral element and N source had significant effect on the growth rate of the mycelia.The optimum solid culture conditions were glucose,bean cake powder,pH and lacking KCl in the medium.The growth rate of mycelia was 2.28 mm·d-1 under the optimized cultivation.
【Fund】: 国家“十一五”科技支撑项目(2006BAD19B08 2006BAD19B04);; 国家林业局“948”项目(2006-4-71)共同资助
【CateGory Index】: S646
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