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《Journal of Anhui Agricultural University》 2011-02
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Extraction of flavonoids from Phyllostachys pubescens shoot

WANG Wen-wen,YUAN Yi,YUAN Cai-hong,WU Jing-wen(School of Life Sciences,Anhui Agricultural University,Hefei 230036)  
Flavonoids from Phyllostachys pubescens shoot were extracted by methods of water,ethanol backflow and ultrasonic wave extractions.The optimum extraction condition was obtained by single factor analy-sis and orthogonal test.The results showed that the yield of flavonoids was 0.706% by water extraction under the conditions of 60℃,1∶30 solid-liquid ratio,pH 9.0 and 1.5 h.The order of the factors which affected water ex-traction was soild-liquid ratio extraction temperature extraction time pH.The yield of flavonoids was 1.063% by the method of ethanol backflow under the conditions of 1∶40 solid-liquid ratio,60℃,70% ethanol and 2.0 h.The order of the factors which affected the ethanol backflow extraction was soild-liquid ratio extrac-tion temperature extraction time volume fraction of ethanol solution.The yield of flavonoids was 0.797% by methods of ultrasonic wave under the conditions of 1∶40 solid-liquid ratio,50℃,70% ethanol and 30 min.The order of the factors which affected the ultrasonic wave extraction was soild-liquid ratio extraction temperature extraction time volume fraction of ethanol solution.
【Fund】: “十一五”国家科技支撑项目(2007DAQ01049 2008BADA9B04)资助
【CateGory Index】: S795.708
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