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《Journal of Anhui Agricultural University》 2011-04
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Establishment of an indirect ELISA method using a infectious bursal disease virus VP2 protein as antigen

SONG Jun,HUANG Cheng-bin,SHAN Xue-qin,PAN Ling(School of Animal Science and Technology,Anhui Agricultural University,Hefei 230036)  
The VP2 protein of infectious bursal disease virus(IBDV) is highly conserved and could be used as diagnostic antigen in serological test.In this study,a VP2 protein gene was cloned and expressed in Escherichia coli.The expressed protein was used to establish an indirect ELISA method.The optimal coating concentration of antigen was amounted to 174.67 ng of VP2 protein per well,and the serum sample for testing was diluted to 1:40.A total of 979 serum samples originated from different areas in Anhui province were detected by this indirect ELISA,and the positive rate was 39.73%.The results confirmed that indirect ELISA was specific to monitor se-rum antibody of IBD.This detection method is quick and easy,and it is deserved to be popularized.
【CateGory Index】: S852.657
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