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《Journal of Anhui Agricultural University》 2012-03
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Establishment and optimization of an ISSR reaction system for Polygonatum cyrtonema Hua using orthogonal design

ZHANG Hong-mei1,SHAO Yuan-hua1,LONG Tian-tian1,XIANG Yan1,WANG Jin2,TANG Feng2(1.School of Forestry and Landscape Agriculture,Anhui Agricultural University,Hefei 230036; 2.International Centre for Bamboo and Rattan,Beijing 100102)  
In this paper,the orthogonal design of five factors(Taq DNA polymerase,buffer(Mg2+),DNA tem-plate,dNTP,and primer) in four levels was used to optimize the ISSR-PCR amplification system on Polygonatum sibiricum.Finally,a satisfactory ISSR(Inter Simple Sequence Repeat) reaction system for Polygonatum sibiricum with desirable repeatability and polymorphic bands was established.It contained 1.0 U Taq DNA polymerase,3.5 mmol?L-1 buffer(Mg2+),80 ng DNA template,0.08 mmol?L-1 dNTP and 0.16 μmol?L-1 primer respectively in a 25 μL ISSR reaction system.The optimal annealing temperature of every prime for ISSR-PCR reaction was proposed by gradient PCR with established ISSR reaction,the optimal annealing temperature was 54.8℃ for primer UBC841,and different primers had different annealing temperatures.This optimized ISSR reaction system would provide the basis for the analysis of classification,map construction and gene localization of Polygonatum sibiricum.
【Fund】: 国家林业公益性行业科研专项(201004055)资助
【CateGory Index】: S567.239
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Chinese Journal Full-text Database 1 Hits
1 XIU Jiang-fan ZHANG Chun-lin CHEN Han-bin (Department of Biology,Guiyang Medical College,Guiyang,Guizhou 550004,China);Establishment and Optimization of an ISSR Reaction System for Simulium quinquestriatum[J];Guizhou Science;2013-03
Chinese Journal Full-text Database 9 Hits
1 HU Su1,3,WANG Yong-qing2,TAO Lian2(1.College of Forestry,Sichuan Agricultural University,Ya'an 625014,China;2.College of Horticulture, Sichuan Agricultural University,Ya'an 625014,China;3.Institute of Biotechnology and Nuclear Technology,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China);Establishment and optimization of an ISSR reaction system for Oxalis triangularis[J];Acta Prataculturae Sinica;2011-05
2 FU Yan1,LUO Nan1,YANG Qin1,2,WANG Yong-qing1,DENG Qun -xian1,LI Jun -qiang1,QIN Hong -mei1,RUAN Guang-lun3(1College of Forestry and Horticulture,Sichuan Agricultural University,Ya'an,Sichuan 625014 China;2College of Environmental and Life Science,Kaili University,Kaili,Guizhou 55600 China;3Fruit Office of Fuling Area of Chongqing,Chongqing 408000 China);Establishment and optimization of ISSR reaction system for species of Eriobotrya[J];Journal of Fruit Science;2009-02
3 ZHOU Shou biao,ZHANG Xiao ping, ZHANG Ding cheng,SHAO Jian zhang ( Department of Biology, Anhui Normal University , Wuhu 241000,China );A cladistic analysis of Polygonatum from Anhui[J];Guihaia;2000-04
4 WANG Jie-ming XIANG Yan WU Da-qiang SUN Zhi-juan CAI Cheng(College of Forest and Garden,Anhui Agricultural University,Hefei,Anhui 230036);ESTABLISHMENT OF AN ISSR REACTION SYSTEM FOR POPLAR AND OPTIMIZATION USING ORTHOGONAL DESIGN[J];Journal of Nuclear Agricultural Sciences;2007-05
5 He Zhengwen Liu Yunsheng Chen Lihua Cao Meihong(Department of Neurosurgery, Xiangya Hospital, Hunan Medical University, Changsha 410008)Xia Jiahui(The State Key Lab. of Medical Genetics of China, Changsha 410078);Orthogonal design-direct analysis for PCR optimization[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;1998-04
6 FANG Zhichao1,2,HUANG Jianfeng2,GAO Aiping2 1 College of Horticulture and Forestry,Hainan University,Haikou,Hainan 570208,China 2 Tropical Crops Genetic Resources Institute,CATAS,Danzhou,Hainan 571737,China;Optimization of ISSR Reaction System for Mango[J];Chinese Journal of Tropical Crops;2011-02
7 LIN Ping~1,ZHANG Han-guo~2,XIE Yun-hai~2(1.Forestry Research Institute of Heilongjiang Province,Harbin 150040;2.Department of Forestry,Northeast Forestry University,Harbin 150040,China);Study on Optimization for ISSR Reaction System of Larix using Orthogonal Design[J];Biotechnology;2005-05
8 Li Fangying1 Huang Yanjing2 Wu Shaohua2 Lai Zhongxiong2 ( 1College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou 350002; 2 College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002);Establishment and Optimization of ISSR System in Bougainvillea[J];Biotechnology Bulletin;2010-07
9 ZHOU Ye1,WANG Run-ling1,TANG Cheng1,AN Shi-zhi1,YAO Zhi2(1.College of Pharmacy;2.Department of Immunology,Tianjin Medical University,Tianjin 300070,China);Identification of Polygonatum sibiricum and P.cirrhifolium by ISSR[J];Journal of Tianjin Medical University;2006-02
Chinese Journal Full-text Database 10 Hits
1 WANG Jia et al(College of Landscape Architecture,Beijing Forestry University,Beijing 100083);Study on Optimization for ISSR Reaction System of Peony with Orthogonal Design[J];Journal of Anhui Agricultural Sciences;2006-24
2 YI Dao-sheng et al(Huo Ying-dong College of Bioengineering,Shaoguan College,Shaoguan,Guangdong 512005);Multiplex PCR Detection and Typing of Porcine Circovirus[J];Journal of Anhui Agricultural Sciences;2007-32
3 ZHANG Tao et al(College of Life Science and Technology,University of Electronic Science and Technology,Chengdu,Sichuan 610054);Optimization for ISSR Reaction System of Hordeum vulgare subsp.Vulgare with Orthogonal Design[J];Journal of Anhui Agricultural Sciences;2007-32
4 LIU Su-xia et al(Henan Normal University,Xinxiang,Henan 453007);Study on Optimization for RAPD Reaction System and Genetic Relationship in Punica granatum[J];Journal of Anhui Agricultural Sciences;2007-36
5 HUANG Zi-feng et al(Dongguan Institute of Agricultural Seeds in Guangdong Province,Dongguan,Guangdong 523063);Optimization of RAPD Reaction System for Lilium formolongi[J];Journal of Anhui Agricultural Sciences;2008-18
6 YI Qiong et al(Biotechnology Research Center,Kunming University of Science and Technolgy,Kunming,Yunnan 650224);Analysis on Genetic Relationships among 8 Main Pear Cultivars in Yunnan Province Based on ISSR Molecular Markers[J];Journal of Anhui Agricultural Sciences;2009-02
7 WANG Wen-feng et al(College of Forestry Science,Gansu Agricultural University,Lanzhou,Gansu 730070);Establishment and Optimization of ISSR-PCR Reaction System for Picea crassifolia kom.[J];Journal of Anhui Agricultural Sciences;2009-21
8 QU Yi-tao et al (Anhui Agricultural University, Hefei, Anhui 230036);Establishment and Optimization of RAPD-PCR System in Trichosanthes kirilowii Maxim[J];Journal of Anhui Agricultural Sciences;2010-13
9 WANG Zhen et al(College of Life and Environmental Sciences,Minzu University of China,Beijing 100081);Optimization for ISSR-PCR Reaction System on Artemisia frigida Willd. Using Orthogonal Design[J];Journal of Anhui Agricultural Sciences;2010-15
10 WANG Fang et al(Experiment Administration Center,Zhongzhou University,Zhengzhou,Henan 450044);Sequence-related Amplified Polymorphism Markers and Their Application in Plant research[J];Journal of Anhui Agricultural Sciences;2011-23
China Proceedings of conference Full-text Database 5 Hits
1 LONG Zhi-jian,HAN Guo-hui,DANG Jiang-bo,LIANG Guo-lu,XIANG Su-qiong~* (College of Horticultural and Landscape Architecture,Southwest University,Chongqing 400716);Genetic diversity of loquat analysed by SSR[A];[C];2011
2 HAN Guo-hui,WANG Wei-xing,XIANG Su-qiong,JIA zhi-gang,GUO Qi-gao,QIN Guo-xin, HE Qiao,LI Xiao-lin,LIANG Guo-lu~* (Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education/College of Horticulture and Landscape Architecture.Southwest University,Chongqing 400715 China);Establishment and optimization of SCoT system in polyploidy loquats[A];[C];2011
3 SHI Qian-qian WANG Yan ZHOU Lin (Research Institute of Forestry,CAF,SFA Key of Breeding and Cultivation,Beijing 100091);Optimization of SRAP-PCR System and Selection of Primers for Paeonia suffruticosa Based on Capillary Electrophoresis[A];[C];2011
4 ZHENG Yi-qi~(1,2) WANG Zhi-yong~2 GUO Hai-lin~2 XUE Dan-dan~2 LIU Jian-xiu~(2*) (1.Department of Horticulture Nanjing Agricultural University Nanjing 210095 China; 2.Institute of Botany Jiangsu Province and Chinese Academy of Sciences Nanjing 210014 China);Optimization of SRAP-PCR System on centipedegrass Using Orthogonal Design and Selection of Primers[A];[C];2007
5 ;Optimization for ISSR Reaction System of jujube tree by Orthogonal Design[A];[C];2009
Chinese Journal Full-text Database 10 Hits
1 DANG Kang et al (College of Life Science,Northwest A & F University,Yangling,Shaanxi 712100);Studies on Photosynthetic Characteristics in 3 Species of Polygonatum[J];Journal of Anhui Agricultural Sciences;2006-11
2 TIAN Qi-jian et al(College of Urban and Rural Resources and Programming,Jishou University,Zhangjiajie,Hunan 427000);Primary Report on the Study of Stereoscopic Cultivation Mode of Intercropping Chinese Herb Rhizoma Polygonatum with Corn[J];Journal of Anhui Agricultural Sciences;2007-36
3 OU Ya-li et al(Xingtai Polytechnic College,Xingtai,Hebei 054035);Effects of Photosynthetic Characteristics and Transpiration Rate of Polygonatum sibiricum Red. under Plastic Cover Net[J];Journal of Anhui Agricultural Sciences;2008-24
4 CHEN Yan-ni et al(College of Life Science,Ludong University,Yantai,Shandong 264025);Optimization of the ISSR Reaction System for Solen strictus[J];Journal of Anhui Agricultural Sciences;2009-10
5 LU Li-hua et al (College of Plant Protection,Northwest A&F University,Yangling,Shaanxi 712100);Study on Reproductive Biology of Polygonatum sibiricum Redoute[J];Journal of Anhui Agricultural Sciences;2010-25
6 LV Yang et al(Anhui Acodemy of Scienae and Technonlogg,Hefei Anhui 230031);GC-MS Analysis of Volatile Cmpounds in Different Paits of Polygonatum sibiricum[J];Journal of Anhui Agricultural Sciences;2010-36
7 SONG Shi-wei et al(College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510642);Effects of Fertilizer Application Level on Growth and Photosynthetic Characteristics of Polygonum[J];Journal of Anhui Agricultural Sciences;2011-26
8 NIE liu wang, ZHANG Ding chen, ZHANG Hai jun, ZHANG Ke yun (Department of Biology, Anhui Normal University ,241000,Wuhu, Anhui, China);A STUDY ON ISOZYMES OF 5 SPECIES IN POLYYONATUM MILL[J];JOURNAL OF ANHUI NORMAL UNIVERSITY(NATURAL SCIENCE);1999-01
9 LI Jun-min,JIN Ze-xin(Ecology Institute of Taizhou University,Linhai 317000);Optimization and screening of ISSR reaction system in endangered plant Emmenopterys henryi[J];Journal of Anhui Agricultural University;2006-04
10 ZHANG Wen-jun,LUO Xiang,GUO Yu-hai,DONG Xue-hui,ZHANG Zhi-mei,ZHAI Zhi-xi(College of Agronomy and Biotechnology of China Agricultural University,Beijing 100193);Effects of Nitrogen,Phosphorus and Potassium Fertilization on Yield and Essential Oil Content of Schizonepetate nuifolia[J];Northern Horticulture;2010-16
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Li et al (College of Forestry and Horticultural, Sichuan Agricultural University, Yaan,Sichuan 625014);Study on Plant Regeneration of Oxalis triangularis Subeg.Triang[J];Journal of Anhui Agricultural Sciences;2005-02
2 LI Jian-hui et al (College of Life Science,Hangzhou Teachers College,Hangzhou,Zhejiang 310018);Establishment and Optimization of ISSR Reaction System of Schima superba[J];Journal of Anhui Agricultural Sciences;2006-19
3 PENG Hai et al(College of Medicine and Life Science,Jianghan University,Wuhan,Hubei 430056);Selectivity of Primer Concentration and Annealing Temperature to ISSR Amplified Fragment Length[J];Journal of Anhui Agricultural Sciences;2007-17
4 Sun Yegen(Department of Geography,Anhui Normal University,241000,Wuhu, Anhui,PRC );PRELIMINARY STUDY OF THE KARYOTYPE OF POLYGONATUM ANHUIENSE AND P.LANGYAENSE[J];Journal of Anhui Normal University(Natural Science);1996-02
5 SHAO Jian zhang 1,\ ZHANG Ding cheng 1, SUN Ye gen 2 (1.Department of Biology, Anhui Normal University, 241000, Wuhu, Anhui,China;2.Department of Geography, Anhui Normal University, 241000, Wuhu, Anhui, China);THE BIOLOGICAL CHARICTERISTICS AND THE RESOURCES APPRAISAL OF THE POLYGONATUM FROM ANHUI[J];JOURNAL OF ANHUI NORMAL UNIVERSITY(NATURAL SCIENCE);1999-02
6 LIU Shi-wang,XU Yan-xia,SHI Hong-wu(Life Science and Engineering College,Huanggang Normal University,Huangzhou 438000);Effect of the Ethanol Extract of Oxalis corniculata L on the Growth Curve of Bacterium[J];Northern Horticulture;2007-03
7 CHU Yun-xia1,2,3,TANG Geng-guo4,ZHANG Yong-chun2,3,5,JING Xiang-mi4(1.Horticultural Research Institute,Shanghai Academy of Agricultural Science,Shanghai,201106,China;2.Flower Reasearch Institute,Shanghai Academy of Agricultural Science,Shanghai,201106,China;3.Shanghai Key Lab of Protected Horticutural Technology,Shanghai 201106,China;4.College of Forest Resources and Environment Nanjing Forestry University,Nanjing 210037,China;5.Forestry and Fruit Research Institute,Shanghai Academy of Agricultural Science,Shanghai 201106,China);Study on Optimization for ISSR Reaction System in Chimonanthus Praecox[J];Northern Horticulture;2007-10
8 LIN Zheng-he1, CHEN Rong-bing1*, CHEN Chang-song1, LIN Jin-ke2, HAO Zhi-long2, GAO Shui-lian2, CHEN Lian-cheng2 (1. Tea Research Institute, Fujian Acaedemy of Agricultrual Sciences, Fu’an 355015, China; 2. Horticultural Institute of Fujian Agriculture and Forestry University, Fuzhou 350002, China);Preliminary Application of ISSR Markers in the Genetic Relationship Analysis of Tea Plants[J];Journal of Tea Science;2007-01
9 PENG Yu1,2,HU Jin-yao2,SU Zhi-xian2 (1.Sichuan Provincial Key Laboratory of Environmental Science and Biodiversity Conservation,Research Center of Biodiversity,China West Normal University,Nanchong 637002,China;2.Research Center of Ecology,Mianyang Normal University,Mianyang 621000,China);Research on allelopathic effects of Oxalis corymbosa——an invasive species[J];Acta Prataculturae Sinica;2007-05
10 CHEN Ming-lin,WANG You-bao(College of Life Sciences,Anhui Normal University,Wuhu 241000,China);A comparative study on the physiological properties of the exotic species Oxalis corymbosa and the native species Oxalis corniculata under water stress[J];Acta Prataculturae Sinica;2008-06
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