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《Journal of Anhui Agricultural University》 2014-05
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Comparative analysis of cox1 sequence in three geographic strains of Eimeria tenella in Anhui Province

ZHOU Feiya;WANG Zhongbao;SU Tingting;ZENG Minghua;School of Animal Science and Technology, Anhui Agricultural University;  
To study the genetic relationship of mitochondrial cytochrome c oxidase subunit 1 gene(cox1) in different geographic strains of Eimeria tenella and different Eimeria species, three geographic strains of Eimeria tellena in Anhui were isolated and purified. The cox1 gene was amplified using polymerase chain reaction(PCR) and a partial sequence of the cox1 gene(pcox1)(about 800 bp) was sequenced from the three geographic strains of Eimeria tenella. This cloned pcox1 sequence was compared with the pcox1 gene sequences of Eimeria tenella, Eimeria maxima, Eimeria brunette, and Eimeria acervulina available in the GenBank. The results showed that no difference in the pcox1 sequences was observed in the three geographic strains of Eimeria tellena; however, a distinct difference in different species of Eimeria was detected. The sequence difference between Eimeria tenella and other Eimeria was 9.9%-14.9%. Therefore, the pcox1 sequence could be used as a genetic marker to detect genetic variations in different species of Eimeria. This study would lead to further investigation of population genetics and drug resistance in Eimeria tenella.
【Fund】: 安徽省教育厅基金项目(KJ2012A115)资助
【CateGory Index】: S858.31;S852.7
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