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《Journal of Anhui Agricultural University》 2015-05
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Molecular identification and detection of tobacco anthracnose pathogen

CHAI Xin;XU Yeping;YAO Jian;YU Xiaofeng;ZONG Kai;LI Yunfei;JIANG Tong;School of Plant Protection, Anhui Agricultural University;Technical Center, Anhui Entry-Exit Inspection and Quarantine Bureau;  
The r DNA complete sequence of tobacco anthracnose pathogen was cloned and sequenced. The full length of the r DNA sequence is 2870 bp. Sequence comparison results showed that the rDNA of tobacco anthracnose pathogen shared a high sequence similarity(96.0%-96.2%) with that of Colletotrichum gloeosporioides. Furthermore, from the constructed phylogenetic tree, it was indicated that tobacco anthracnose pathogen and C. gloeosporioides clustered into a separate branch, which indicated that tobacco anthracnose pathogen had the closest relationship with C. gloeosporioides. Therefore, combined with its morphology characteristic, tobacco anthracnose pathogen isolated from tobacco plant in Guizhou could be preliminarily identified as C. gloeosporioides. A pair of specific primer was designed according to the rDNA sequence of tobacco anthracnose pathogen. Not only tobacco anthracnose pathogen could be identified from 8 different pathogens isolated from tobacco plants, but also it could be identified from 7 different anthracnose pathogens. Plants of Nicotiana tabacum were inoculated with tobacco anthracnose pathogen. Using the total DNA extracted from the diseased tissue as a template, a specific band could be amplified by PCR with the specific primer. It was suggested that the method could be applied for the identification and rapid detection of C. gloeosporioides from tobacco plants.
【Fund】: 国家质检总局公益性行业科研专项项目(201210071)资助
【CateGory Index】: S435.72
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【Citations】
Chinese Journal Full-text Database 9 Hits
1 JIA Yu1,ZHENG Cui-mei1,ZHANG Guang-min1,CHEN Xiu-ting2, WEN Liang2,TIAN Fu-hai3,YANG Yong-hua3(1.College of Plant Protection,Shandong Agricultural University,Taian 271018,China; 2.Mengyin Tobacco Company,Mengyin 276200,China;3.Yishui Tobacco Company,Yishui 276400,China);Identification and Molecular Detection of Colletotrichum nicotianae Pathogen from Main Tobacco Fields in Shandong Province[J];Shandong Agricultural Sciences;2012-04
2 Wu Wenping; Zhang Zhiming(Department of Plant Protection, Hebei Agricultural University);Study on Taxonomy of Genus Colletotrichum Cda.Ⅱ. Division of Species[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1994-02
3 Wu Wenping;Zhang Zhiming;Li YuQing(Department of Plant Protection.Hebei Agricultural University, Baoding 071001);Studies on Taxonomy of Genus Colletotrichum Cda.Ⅴ.Species with stright conidia in Hebei[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1995-03
4 LI Dong-sheng1,2,ZHANG Yi-yang1(1.Research Center of Tobacco Engineering and Technology,HNAU,Changsha 410128,PRC;2.Lingling Branch of Yongzhou Tobacco Corporation,Yongzhou 425100,PRC);Study on Morphology and Pathogenicity of Tobacco Anthracnose[J];Hunan Agricultural Sciences;2009-05
5 YAN Miao-miao,WEI Guang-cheng,PAN Xiao-hong,MA Huai-lei,LI Wei-zhen Yantai Campus of Binzhou Medical College,Yantai 264003;A Method Suitable for Extracting Genomic DNA from Animal and Plant——Modified CTAB Method[J];农业科学与技术(英文版);2008-02
6 XING Hong-mei1,2 DING Ping2 WANG Ke-rong2 ZHOU Xiao-yun1 1.Guangzhou Flower Research Center,Guangzhou 510360,China; 2.College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China;Molecular detection of Colletotrichum destructivum in Anthurium andraeanum[J];Journal of Huazhong Agricultural University;2011-05
7 ZHANG Zhong-yi, LIU Yun-long, LIU Yuan, CAO Wen-guang (Key Laboratory for Plant Pathology of Yunnan Province, Kunming, Xinjiang 650201, China);Taxonomy of Colletotrichum Corda in China Ⅰ[J];Journal of Shihezi University(Natural Science);2004-S1
8 Yang Panchuan (Department of Plant protection, Yunnan Agricultural University, Kunming);Studies on the Morphological and Physiological Characters of Colletotrichum Gloesporioedes (penz) Sacc from Yunnan[J];Journal of Yunnan Agricultural University;1992-04
9 YANG La-ying,HUANG Hua-ping,TANG Fu-run,HU Mei-jiao,ZHANG Shi-qing,HUANG Jun-sheng(Environmental and Plant Protection Institution,State Key Laboratory of Tropical Crop Biotechnology,Chinese Academy of Tropical Agricultural Science,Haikou 571101,China);Rapid molecular identification and detection of Colletotrichum musae with species-specific primers based on the internal transcribed spacer(ITS) region[J];Acta Phytopathologica Sinica;2006-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 JIA Yu1,ZHENG Cui-mei1,ZHANG Guang-min1,CHEN Xiu-ting2, WEN Liang2,TIAN Fu-hai3,YANG Yong-hua3(1.College of Plant Protection,Shandong Agricultural University,Taian 271018,China; 2.Mengyin Tobacco Company,Mengyin 276200,China;3.Yishui Tobacco Company,Yishui 276400,China);Identification and Molecular Detection of Colletotrichum nicotianae Pathogen from Main Tobacco Fields in Shandong Province[J];Shandong Agricultural Sciences;2012-04
2 CHEN Jian-shan et al (Environment and Plant Protection College,SCUTA,Danzhou,Hainan 571737);Application of ITS Sequences in Fungi Classification and Identification[J];Journal of Anhui Agricultural Sciences;2007-13
3 SONG Xiao-fei et al (Hebei Normal University of Science and Technology,Changli,Hebei 066600);Study on Extraction of Genomic DNA and Optimization of AFLP Techniques of Cucumber(Cucumis sativus L.)[J];Journal of Anhui Agricultural Sciences;2009-29
4 ZHANG Shu-mei et al(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin,Helongjiang 150010);Advances in Molecular Diagnosis of Plant Fungal Pathogens[J];Journal of Anhui Agricultural Sciences;2010-02
5 SHEN Jie et al(School of Life Sciences,Chongqing Normal University,Chongqing 400047);Comparison on Methods for Extracting DNA from Pteridophyta[J];Journal of Anhui Agricultural Sciences;2010-04
6 ZHAO Zhen-ling et al(Medicinal Plant Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650231);Pathogen Identification of the Anthracnose of Ligusticum chuanxiong Hort.and the Inhibitory Effect of Four Fungicides on the Pathogen[J];Journal of Anhui Agricultural Sciences;2010-12
7 CHEN Xue-yan(School of Biological Science and Engineering,Shaanxi Institute of Technology,Hanzhong,Shaanxi 723001);Rapid DNA Extraction Method from Amorphophallas. konjac and ISSR-PCR Amplification[J];Journal of Anhui Agricultural Sciences;2010-19
8 LUO Zhi-wen et al(College of Environmental and Plant Protection,Hainan University,Danzhou,Hainan 571737);Pathogen Identification and Biological Characteristics of Pineapple Anthracnose[J];Journal of Anhui Agricultural Sciences;2010-34
9 DING Fei1,WANG Qian2,JIANG Lei1,LI Rui1,JIA Lin1,JIANG Tong1(1.School of Plant Protection,Anhui Agricultural University,Hefei 230036;2.Xiangshan Bureau of Agroforestry and Water Conservancy,Huaibei 235000);First report of pathogen identification of tomato leaf curl disease in Huaibei,Anhui Province[J];Journal of Anhui Agricultural University;2010-03
10 LIU Fang1,LIN Ying-ren1,WANG Shi-juan1,GAO Xiao-ming2,CHEN Li1,JIA Guo-jun2(1.School of Forestry and Landscape Architecture;2.School of Life Science,Anhui Agricultural University,Hefei,Anhui 230036,China);ITS sequence analysis of intraspecific genetic diversity of Hypoderma rubi[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2011-01
China Proceedings of conference Full-text Database 7 Hits
1 XU Jing FAN Yan-ping (College of Hoticulture,South China Agriculture University,Guangzhou 510642);Studies on the Biological Characteristics in Anthracnose of Dracaena sanderiana[A];[C];2007
2 JING Yu~1 ZHAO Yan~1 ZHANG Rong~2 ZHANG Tao~3 LAN Jian-qiang~1 HE Yong-hong~1 ZHANG Zhong-yi~1 (1.Key Laboratory for Plant Pathology of Yunnan Province,YUN,Kunming 650201;2.Northwestem Sci- Tech University of Agriculture & Forestry,Yangling,shaanxi,712100;3.Kunming Research Institute of Edlible Fungi,Kunming,650223);Taxonomy of Colletotrichum Corda in China Ⅴ[A];[C];2008
3 MA Yan~1 HE Chun-ying~1 LUO Qiong~1 ZHAO Yan~1 ZENG Qian-chun~1 ZHANG Tao~1 ZHANG Zhong-yi~1 (1.National center for Agro-biodirersity,Yunnan Agricultural university,Kunming 650201;2.Kunming Research Institute of Edlible Fungi,Kunming,650223);Taxonomy of Colletotrichum Corda in China Ⅳ[A];[C];2008
4 Qu Qian~1 Lan Jian-qiang~1 Zhang Tao~2 Gong Liwei~2 KANG Zhen-sheng~3Zhang Zhonhyi~1 (1.Faculty of Plant Protection,Yunnan Agricultural University,Kunming 650201; 2.Kunming Research Institute of Edlible Fungi,Kunming 650223; 3 Faculty of Plant Protection,Northwest A & F university,Yangling,shanxi,712100);Taxonomy of Colletotrivhum Corda in China Ⅶ.[A];[C];2010
5 ZHENG Xiao-lan~1 GONG Li-wei~2 ZHANG Tao~2 TZEAN Sheng-shung~3 ZHANG Zhong-yi~4 LIU YUN-long~4 (1.Environment and Plant Protectin Research Institute,Chinese Academy of Tropics Agricutural Sciences,Danzhou 571737; 2.Kunming Research Institute of Edlible Fungi,Kunming 650223; 3.Department of Plant Pathology,Taiwan University,Taibei 10617; 4.Faculty of Plant Protection,Yunnan Agricultural University,Kunming 650201);Two New Species of Colletotrichum in China-Ⅳ[A];[C];2011
6 ZHANG Tao~1 LAN Jian-qiang~3 GONG Li-wei~1 TZEAN Shian-ahong~2 WU Jia~3 ZHANG Zhong-yi~3 (1.Kunming Research Institute of Edlible Fungi,Kunming 650223; 2.Department of Plant Pathology,Taiwan University,Taibei 10617; 3.Faculty of Plant Protection,Yunnan Agricultural University,Kunming,Yunnan 650201);Two New Species of the Genus Gloeosporium from China Ⅰ[A];[C];2011
7 LI Xiao-ling~1 TZEAN Shian-shong~2 ZHANG Tao~1 ZHANG Zhong-yi~3 (1.Kunming Research Institute of Edlible Fungi,Kunming 650223; 2.Department of Plant Pathology,Taiwan University,Taibe,106; 3.Faculty of Plant Protection,Yunnan Agricultural University,Kunming 650201);Two New Species of Colletotrichum Corda in China-Ⅴ[A];[C];2011
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 JIA Yu1,ZHENG Cui-mei1,ZHANG Guang-min1,CHEN Xiu-ting2, WEN Liang2,TIAN Fu-hai3,YANG Yong-hua3(1.College of Plant Protection,Shandong Agricultural University,Taian 271018,China; 2.Mengyin Tobacco Company,Mengyin 276200,China;3.Yishui Tobacco Company,Yishui 276400,China);Identification and Molecular Detection of Colletotrichum nicotianae Pathogen from Main Tobacco Fields in Shandong Province[J];Shandong Agricultural Sciences;2012-04
2 CHEN Jian-shan et al (Environment and Plant Protection College,SCUTA,Danzhou,Hainan 571737);Application of ITS Sequences in Fungi Classification and Identification[J];Journal of Anhui Agricultural Sciences;2007-13
3 ZHANG Hui-juan et al(Nanjing Research Institute for Agricultural Mechanization,Ministry of Agriculture,Nanjing,Jiangsu 210014);General Situation and Development Strategy of Tobacco Production in China[J];Journal of Anhui Agricultural Sciences;2008-32
4 Wu Wenping;Zhang Zhiming;Li YuQing(Department of Plant Protection.Hebei Agricultural University, Baoding 071001);Studies on Taxonomy of Genus Colletotrichum Cda.Ⅴ.Species with stright conidia in Hebei[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1995-03
5 LIU Xue min,CHANG Wen (Department of Plant Protection,Northeast Agricultural University,Harbin,Heilongjiang, 150030,PRC) LI Da zhuang (Binxian Tobacco Company,Binxian,Heilongjiang 150400);Researching advance of tobacco brown spot caused by Alternaria alternata[J];JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY;2000-01
6 CHEN Zhi-min,WU Hao-xin,ZENG Hui-fang,ZHANG Shao-sheng(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Identification of Pythium aphanidermatum isolated from tobacco field and the screening of Trichoderma spp. for antagonistic agent[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2009-01
7 JIANG Xi-wang1,BAO Man-zhu1,XUE Dong1,ZHOU Da-hong2 (1. HuaZhong Agricultural University, Wuhan 430070, China; 2. Flower Nursery in Hannan District, Wuhan 430010, China);Diseases on Chrysanthemum in China[J];Journal of Gansu Agricultural University;2002-02
8 WANG Wanneng, XIAO Chonggang (College of Plant Protection, Southwest Agricultural University, Chongqing 400716, China);Progress of study on integrated management of tobacco black shank[J];Guangxi Agricultural Science;2003-02
9 YAN Zhu-Huai1,CAO Na2(1.Longling Distract Tobacco Company,Baoshan 678300,China;2.Baoshan Tobacco Company of Yunnan Province,Baoshan 678000,China);Research Progress of Colletotrichum capsici of Tobacco and its Control Measures[J];Journal of Hebei Agricultural Sciences;2008-08
10 SUN Ji-ping,LI Xue-jun,WU Zhao-hui,SUN Huan(Tobacco Research Center of Henan Academy of Agricultural Sciences/Tobacco Research Institute of Henan Tobacco Company, Xuchang 461000,Henan,China);Progress on Tobacco Black Shank Disease[J];Hubei Agricultural Sciences;2011-16
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