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Research on Purity and Features of Superoxide Dismutase of Mulbery Leaf

Yuan Yi Li Chun Zheng Xiaoqing (Bioengineering Department,Anhui Agricultural University,Hefei 230036)  
The mulbery leaf SOD can inhibit autoxidation of epinephrine and NBT photoreduetion.The enzyme quatity can be determined from the amount that inhibits the epinephrine autoxidation.The enzymatic activity is inhibited by KCN and is not affected by chioroform-enthanol,which indicates that mulbery SOD is CuZn-SOD.The crude extract presents two bands using polyacrylamide gel electrophoresis and NBT enzymatic activity stain.The enzymatic activity (units/mg) is increased by 2 7 times after the crude extract was purified,and the molecular weight is 35 000.
【CateGory Index】: Q946.5
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