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Analysis on Serum and Body Surface Slime Proteins and their Antigenic Relationship Among Carassius auratus and Eight other Fish Species

Yu Weiyi Zhu Cuilan Zu Guozhang Li Jinnian Yang Qichao Lei Zhiwen Xia Chengyun (Department of Animal Science,Anhui Agricultural University,Hefei 230036)  
The relationship of molecules and cross antigens of the serum and body surface slime proteins among Carassius auratus and eight otheir fish species under three orders and five families was analysed by SDS-PAGE,gel diffusion and immunoelectrophoresis.The bands of the serum and body surface slime proteins of the tested species in the map of SDS-PAGE showed no significant difference.The antibodies to C.auratus' serum proteins could react with both its serum and its body surface slime proteins,producing three and two precipitation bands respectively in the gel diffusion,at least ten precipitation bands in the immunoelectrophoresis.In addition,except those of O.argus and M.albus,the antibodies could bind those of other six species.These results showed that there existed the cross antigens in serum and body surface slime among various fish species,which corresponded with their relationship to each other.
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