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《Journal of Anhui Technical Teachers College》 2005-03
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A New Phloroglucinal Spectrophotometric Method for Determination of Wheat Pentosan and Its Applicactions

SHI Xia-qing( Department of Botany Science,Anhui Science and Technology University,Fengyang 233100 ,China)  
Water soluble pentosans can be satisfactorily determined by phloroglucinal spectrophotometric method.But most portion of pentosans in wheat are water insoluble. They differ from that of water soluble pentosans.Therefore, the method originally used in water soluble pentosans determination is unsuitablly used for present wheat pentosans determination. In this paper, after wheat samples being practically used in analysis and analytical conditions being fully optimized , a new method for phloroglucinal spectrophotometric determination of wheat pentosans is proposed. The linear range is 15.4~34.6 mg/L; the linear equation is y = -226.0 + 989.8x (absorption specpra difference) with r = 0.9915; the detection limit is 0.03 mg/L; the recovery of standard pentosan addition is 92.1%~102.6%; the relative standard deviation for 6 parallel determinations of wheat samples with 4.76% pentosan content is 2.18%. The method has been successfully applied for analyzing more than 260 kinds of wheat samples in respects of quality studies and breeding studies with satisfactory results.
【Fund】: 安徽科技学院自然基金项目(2003ZY011)。
【CateGory Index】: S512.1
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